Molecular approaches to enhance astaxanthin biosynthesis; future outlook: engineering of transcription factors in
            <i>Haematococcus pluvialis</i>

Kayani, Sadaf‐Ilyas; Rahman, Saeed-ur; Shen, Qian; Cui, Yi; Liu, Wei; Hu, Xinjuan; Zhu, Feifei; Huo, Shuhao

doi:10.1080/07388551.2023.2208284

Cited by 3 publications

(3 citation statements)

References 136 publications

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“…Although the TFE has been widely applied in genetic engineering modification of yeast and microalgae, various TFs regulating microbial astaxanthin synthesis have been continuously being discovered; the genetic modification of astaxanthin biosynthesis based on TFE technology has not been reported ( Grama et al, 2022 ). The potential of TFE to increase astaxanthin productivity in H. pluvialis has been recognized and proposed ( Kayani et al, 2023 ). In this study, a biochemical and genetic strategy based on hormone treatment and TFE is applied in the astaxanthin-producing yeast P. rhodozyma for the first time and simultaneously increased its cell growth and astaxanthin synthesis, confirming that our strategy is efficient and lay the foundation for microbial astaxanthin production.…”

Section: Discussionmentioning

confidence: 99%

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

Jia,

Chen,

et al. 2024

Front. Microbiol.

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Astaxanthin has multiple physiological functions and is applied widely. The yeast Phaffia rhodozyma is an ideal source of microbial astaxanthin. However, the stress conditions beneficial for astaxanthin synthesis often inhibit cell growth, leading to low productivity of astaxanthin in this yeast. In this study, 1 mg/L melatonin (MT) could increase the biomass, astaxanthin content, and yield in P. rhodozyma by 21.9, 93.9, and 139.1%, reaching 6.9 g/L, 0.3 mg/g DCW, and 2.2 mg/L, respectively. An RNA-seq-based transcriptomic analysis showed that MT could disturb the transcriptomic profile of P. rhodozyma cell. Furthermore, differentially expressed gene (DEG) analysis show that the genes induced or inhibited significantly by MT were mainly involved in astaxanthin synthesis, metabolite metabolism, substrate transportation, anti-stress, signal transduction, and transcription factor. A mechanism of MT regulating astaxanthin synthesis was proposed in this study. The mechanism is that MT entering the cell interacts with components of various signaling pathways or directly regulates their transcription levels. The altered signals are then transmitted to the transcription factors, which can regulate the expressions of a series of downstream genes as the DEGs. A zinc finger transcription factor gene (ZFTF), one of the most upregulated DEGs, induced by MT was selected to be overexpressed in P. rhodozyma. It was found that the biomass and astaxanthin synthesis of the transformant were further increased compared with those in MT-treatment condition. Combining MT-treatment and ZFTF overexpression in P. rhodozyma, the biomass, astaxanthin content, and yield were 8.6 g/L, 0.6 mg/g DCW, and 4.8 mg/L and increased by 52.1, 233.3, and 399.7% than those in the WT strain under MT-free condition. In this study, the synthesis and regulation theory of astaxanthin is deepened, and an efficient dual strategy for industrial production of microbial astaxanthin is proposed.

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Section: Discussionmentioning

confidence: 99%

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

Jia,

Chen,

et al. 2024

Front. Microbiol.

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“…However, the regulatory mechanism of carotenoid metabolism in algae is still seriously lacking and even in some carotenoidrich algae such as the β-carotene-accumulating D. salina 6 and the astaxanthin-accumulating H. pluvialis. 134 The current understanding of the functions of regulators including transcription factors and regulatory proteins in algae is still limited, mainly because the genetic background of most algae, especially eukaryotic algae, is not well understood. For the genetic manipulation of algae at present, very few algal species can perform genetic manipulation efficiently, except for the model species C. reinhardtii.…”

Section: Future Challenges and Prospectsmentioning

confidence: 99%

“…salina and the astaxanthin-accumulating H. pluvialis . The current understanding of the functions of regulators including transcription factors and regulatory proteins in algae is still limited, mainly because the genetic background of most algae, especially eukaryotic algae, is not well understood.…”

Section: Future Challenges and Prospectsmentioning

confidence: 99%

Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

Liang,

2023

J. Agric. Food Chem.

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Astaxanthin: Past, Present, and Future

Nishida,

Berg,

Shakersain

et al. 2023

Marine Drugs

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Astaxanthin (AX), a lipid-soluble pigment belonging to the xanthophyll carotenoids family, has recently garnered significant attention due to its unique physical properties, biochemical attributes, and physiological effects. Originally recognized primarily for its role in imparting the characteristic red-pink color to various organisms, AX is currently experiencing a surge in interest and research. The growing body of literature in this field predominantly focuses on AXs distinctive bioactivities and properties. However, the potential of algae-derived AX as a solution to various global environmental and societal challenges that threaten life on our planet has not received extensive attention. Furthermore, the historical context and the role of AX in nature, as well as its significance in diverse cultures and traditional health practices, have not been comprehensively explored in previous works. This review article embarks on a comprehensive journey through the history leading up to the present, offering insights into the discovery of AX, its chemical and physical attributes, distribution in organisms, and biosynthesis. Additionally, it delves into the intricate realm of health benefits, biofunctional characteristics, and the current market status of AX. By encompassing these multifaceted aspects, this review aims to provide readers with a more profound understanding and a robust foundation for future scientific endeavors directed at addressing societal needs for sustainable nutritional and medicinal solutions. An updated summary of AXs health benefits, its present market status, and potential future applications are also included for a well-rounded perspective.

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Molecular approaches to enhance astaxanthin biosynthesis; future outlook: engineering of transcription factors in Haematococcus pluvialis

Cited by 3 publications

References 136 publications

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

The enhancement of astaxanthin production in Phaffia rhodozyma through a synergistic melatonin treatment and zinc finger transcription factor gene overexpression

Involvement of Transcription Factors and Regulatory Proteins in the Regulation of Carotenoid Accumulation in Plants and Algae

Astaxanthin: Past, Present, and Future

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