2000
DOI: 10.1017/s0953756200003476
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Molecular approaches and the concept of species and species complexes in lichenized fungi

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Cited by 112 publications
(87 citation statements)
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“…Species concept and species delimitation have been recently reviewed in a broad sense by de Queiroz (2007) and in lichens by Grube & Kroken (2000), these topics, therefore, shall not be revisited in the present work. The presence of lineages of cryptic species poses a major problem that affects not only taxonomy, but also other fields of biology such as biodiversity research and conservation policy (Bickford & al., 2007).…”
Section: Recognition Of Cryptic Species Lineagesmentioning
confidence: 99%
“…Species concept and species delimitation have been recently reviewed in a broad sense by de Queiroz (2007) and in lichens by Grube & Kroken (2000), these topics, therefore, shall not be revisited in the present work. The presence of lineages of cryptic species poses a major problem that affects not only taxonomy, but also other fields of biology such as biodiversity research and conservation policy (Bickford & al., 2007).…”
Section: Recognition Of Cryptic Species Lineagesmentioning
confidence: 99%
“…Significant growth-rate differences were in accordance (Table 1), as was an ITS minimum p distance of 1.57 Ϯ 0.58% [see supporting information (SI) Methods]. Such differences of these characters are used for delimitation and characterization of fungal species (24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37). We term the species spp.…”
Section: Resultsmentioning
confidence: 82%
“…Although it was not possible to identify races by using these molecular techniques, they did allow determining the pathogen's genetic diversity. Furthermore, ITS sequences have been used extensively to perform molecular analyses of the diversity of phytopathogenic fungi, characterize new fungal species and separate very closely related species or races of the same species (Grube and Kroken, 2000 (Várzea y Marques, 2005). Sin embargo, estos conjuntos de cafetos diferenciales fueron insuficientes para la caracterización de nuevas razas virulentas del hongo, por lo que, se plantearon nuevas técni-cas para determinar su variabilidad, como el uso de marcadores moleculares (Nandris et al, 1998) y la secuenciación de los ITS del ADN ribosomal (Cristancho et al, 2007).…”
Section: Abstract: Yellow Rust Its Coffea Spp Haplotypesunclassified
“…Si bien es cierto con estas téc-nicas moleculares no fue posible la identificación de razas, sin embargo, permitieron determinar la diversidad genética del patógeno. Además, las secuencias ITS han sido muy utilizadas en el análisis molecular de la diversidad de hongos fitopatóge-nos, así como en la caracterización de nuevas especies de hongos, y en la separación de especies muy cercanas o de razas de una misma especie (Grube y Kroken, 2000). Su aplicación a H. vastatrix viene ofreciendo nuevos panoramas sobre su diversidad que puede ser utilizada en programas de mejoramiento genético.…”
Section: Abstract: Yellow Rust Its Coffea Spp Haplotypesunclassified