Molecular and physiological study of the artificial maturation process in European eel males: From brain to testis

Peñaranda, David S.; Pérez, Luz; Gallego, Víctor; Jover, M.; Tveiten, Helge; Baloche, S.; Dufour, Sylvie; Asturiano, Juan F.

doi:10.1016/j.ygcen.2009.08.006

Cited by 51 publications

(92 citation statements)

References 84 publications

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“…Parameter yang diamati pada penelitian ini adalah 1) indeks gonadosomatik (IGS), 2) tingkat kematangan gonad mengacu pada klasifikasi Miura dan Miura (2011) dan Peñaranda et al (2010), 3) nisbah kelamin, 4) level testosteron, menggunakan metode enzym-linked immuno assay (ELISA), 5) pertambahan panjang, dan 6) pertambahan bobot,…”

Section: Parameter Pengamatanunclassified

Induced maturation of eel weighed 100–150 gram with PMSG, antidopamine, and 17α-methyltestosterone

Aryani¹,

Sudrajat²,

Carman³

2015

Jurnal Akuakultur Indonesia

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PENDAHULUANIkan sidat di Indonesia belum dimanfaatkan secara optimal karena belum banyak dikenal oleh masyarakat padahal banyak ditemukan di wilayah perairan Indonesia. Indonesia menjadi salah satu negara yang berpotensi mengembangkan produksi ikan sidat di pasaran lokal maupun internasional. Salah satu jenis ikan sidat yang cukup potensial di Indonesia adalah Anguilla bicolor bicolor. Budidaya ikan sidat di Indonesia masih terbatas sampai di segmen pembesaran ABSTRACTMarketed eel Anguilla bicolor bicolor is commonly produced from larvae rearing activity whose broodstocks and larvae are caught from the nature. Supply of eel broodstock is restricted by its life cycle and uncertain size variation of mature male and female. This study was aimed to evaluate the effect of hormonal induction through injection to enhance masculinization and gonadal maturation of eel at the weight of 100-150 g. The experiment used completely random design with the use of combination pregnant mare serum gonadotropin (PMSG), antidopamine (AD), dan 17α-methyltestosterone (MT), which were (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/ kg MT), and P5 (control; without hormonal treatment). The result showed that an increasing of fish length along with fish weight were performed by treatment P4 and P3. The highest gonadosomatic index value was obtained by treatment P3 (1,3030±0,24262). Based on gonadal histology analysis, 2 nd phase of spermatogonia development was found in P3 in week-8. The highest testosterone level was obtained by treatment P3, followed by P4, P2, and P1 in week-4. Combination of 10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT could enhance masculinization and gonadal maturation of eel in eight weeks of rearing period.Keywords: gonadal maturation, Anguilla bicolor bicolor, PMSG, AD, MT ABSTRAKIkan sidat Anguilla bicolor bicolor yang dipasarkan pada umumnya merupakan hasil usaha pembesaran yang benih dan induknya masih diperoleh dari alam. Penyediaan induk ikan sidat terkendala dengan siklus hidup dan variasi perbedaan ukuran induk ikan sidat jantan dan betina matang gonad yang belum pasti. Penelitian ini bertujuan untuk mengkaji peran induksi hormonal yang disuntikkan pada ikan sidat dalam mempercepat proses pematangan gonad ikan sidat ukuran 100-150 g. Metode penelitian yang digunakan adalah metode eksperimen rancangan acak lengkap dengan kombinasi pregnant mare serum gonadotropin (PMSG), antidopamin (AD), dan 17α-metiltestosteron (MT) sebagai berikut P1 (10 IU/kg PMSG + 0,01 mg/kg AD), P2 (20 IU/kg PMSG + 0,01 mg/kg AD), P3 (10 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), P4 (20 IU/kg PMSG + 0,01 mg/kg AD + 150 µg/kg MT), dan P5 (kontrol; tanpa perlakuan hormon). Hasil penelitian menunjukkan peningkatan pertambahan panjang seiring dengan pertambahan bobot diperoleh dari perlakuan P4 dan P3. Indeks gonadosomatik tertinggi diperoleh dari perlakuan P3 (1,3030±0,24262). Hasil histologi gonad ditemukan perkembangan...

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Section: Parameter Pengamatanunclassified

Induced maturation of eel weighed 100–150 gram with PMSG, antidopamine, and 17α-methyltestosterone

Aryani¹,

Sudrajat²,

Carman³

2015

Jurnal Akuakultur Indonesia

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“…In this study we observed a progressive decrease in male European eel E2 plasma levels during induced spermatogenesis, contrary to the increase observed in experimentally matured female eels . Nevertheless, in both sexes, androgen (testosterone and 11-ketotestosterone) plasma levels increase during induced maturation Peñaranda et al, 2010).…”

Section: Brain and Pituitary Estrogen Receptor Expression Levels Durimentioning

confidence: 99%

“…All samples of brain, pituitary, testis, liver, gill, muscle, spleen, pectoral fin, heart, posterior kidney and head kidney were stored in 0.5 ml of RNAlater (Ambion Inc., Huntingdon, UK) at -20 ºC until extraction of total RNA (Peñaranda et al, 2010). The brain was dissected into five parts: olfactory bulbs, telencephalon, mes-/diencephalon, cerebellum, and medulla oblongata.…”

Section: Fish Maintenance Hormonal Treatments and Samplingmentioning

confidence: 99%

“…It is known that in male fish two progestins: 17α,20β-dihydroxy-4-pregnen-3-one (DHP) and/or 17α,20β,21-trihydroxy-4-pregnen-3-one (20βS) are the maturation-inducing steroids (MIS), mediating the process of sperm maturation and spermiation . In Japanese and European eels, DHP has been proposed to be an essential factor for the meiosis initiation at the beginning of spermatogenesis Peñaranda et al, 2010), but also to regulate final sperm maturation through the increase of pH in the seminal plasma, which induces an increase in intracellular cAMP, making the sperm cells capable of motility and fertilization (Miura et al, 1995). Furthermore, it is known that progestins regulate spermiation in Salmonidae and Cyprinidae (Ueda et al, 1985), increase milt production in Moronidae (Asturiando et al, 2002) and Salmonidae (Baynes et al, 1985), and stimulate sperm motility in Anguillidae (Miura et al, 1995) and Sciaenidae (Tubbs et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In the case of the European eel, the temperature of the hypothetical spawning area is around 20 ºC (Boëtius and Boëtius, 1967), and that is the reason why the maturation of males and females of this species has traditionally been performed in water of that temperature (Peñaranda et al, 2010;Pérez et al, 2009). However, the influence of temperature on the maturation process of the European eel has recently been noted both in females Pérez et al, 2011) and in males (Baeza et al, 2015;Tanaka et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Molecular approaches related to the European eel (Anguilla anguilla) reproductive process

Morini¹

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ACKNOWLEDGEMENTSEn primer lugar quiero dar las gracias a Johnny por haberme dado la oportunidad de realizar este doctorado. Muchas gracias por la ayuda y los buenos consejos prestados. Agradezco mucho tu comprensión y empatía cada vez que he estado malita.Gracias también a Luz, por tu buen humor y tu gran simpatía. Me has hecho sentir muy cómoda desde el principio y además me has convertido en fanática gatuna.David, muchas gracias por haber confiado en mi desde el principio, darme libertad en la realización de los experimentos y haber resuelto todas las dudas que iban surgiendo.Por otra parte quería dedicar un párrafo muy especial a mis primeros amigos españoles, mis preferidos y que a la vez eran mis compañeros de trabajo.Como podéis imaginar siempre es difícil llegar a otro país, lejos de la familia, de los amigos de la infancia y sin hablar el idioma. Vosotros me lo habéis hecho todo más fácil. He aprendido mucho a vuestro lado y he evolucionado mucho gracias a vosotros, de ser sepia (gracias Vic) a casi valenciana con acento francés. Me lo he pasado fenomenal entre las cervecitas del viernes, las excursiones del sábado, las inumerables comidas y cenas, nocheviejas, etc… Rosa, nunca olvidaré nuestra primera salida "Alcampo", tu cara mirándome fijamente mientras cojeabas y sonaba la música de tu móvil!!! Toda una historia… Y en nochevieja, que bien me lo pasé con mi compañera de cubatas y de "baño que pincha", mis vaqueros aún se acuerdan de aquella noche!! Pablo, mi traductor oficial de los Mojinos!! A ver si podemos ir a otro concierto pronto! Y volver a comer crêpes en Paris! Cara-crêpes! Vic, nada más volver de Japón me iniciaste a tu pasión… los pajaritos!! Las orejas de cerdo vinieron mucho más tarde ;-) Me descubriste este mundo y ahora veo los pajaritos con otro ojo (y otra oreja). Tú crees que otro petirrojo nos dará un concierto privado como el de Cazorla??Mamen, no sé ni por dónde empezar… Nunca olvidaré todo lo que has hecho por mí. Recuerdo al principio cuando casi sin conocernos me invitaste a tu cumpleaños junto a toda tu familia!. He disfrutado mucho todos los momentos que hemos pasado juntas estos años (entre las anguilas, el laboratorio, las vacaciones, las fallas, las nocheviejas, los fin des, paseos con bolt, cenas improvisadas...) Creo que he pasado más tiempo contigo que con Richard. Gracias por todo!!! También quiero dar las gracias a una gran cocinera y amiga, llamada Aquagamete, o Pepa, para los íntimos! Me han encantado nuestras comiditas y me ha ayudado mucho tenerte aquí. Además, gracias a ti he podido probar y disfrutar el mejor jamón del mundo! No olvido a los 2 últimos fichados: los vecinos alborayenses Chris y German. Continuaremos disfrutando de nuestras cervecitas y horchatitas después de una partida de volley-playa.Gracias al grupo de nutrición, Miguel, Ana, Silvia, Raquel, Sergio… con mención especial a Guillem por tu ayuda y tu valenciano perfecto (yo tenía complicado el resum en valenciá); y a Nacho, por tu ayuda con la portada y tu paciencia durante el final de mi tesis, ...

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Effects of dopamine and melatonin treatment on the expression of the genes associated with artificially induced sexual maturation in Japanese eel, Anguilla japonica

Byun,

Hyeon,

Hettiarachchi

et al. 2024

J Exp Zool Pt A

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Japanese eel (Anguilla japonica) is a commercially important fish species in Asia. Understanding factors like photoperiod, temperature, and lunar cycles is crucial for successful aquaculture and managing its reproduction. Melatonin and dopamine (DA) are essential for regulating reproduction in vertebrates, including fish. This study investigated the effects of melatonin and DA on the reproductive system of mature male Japanese eels to better understand reproductive regulation in fish. To clarify the effects of these hormones on sexual maturation in eels, a critical stage in the reproductive process, sexual maturation was induced by injecting human chorionic gonadotropin, which stimulates the production of sex hormones. To check the effect of melatonin and DA on sexual maturation, DA, melatonin, and DA + domperidone were intraperitoneally injected into fish from each group (six per treatment) at a dose of 1 mg/kg body weight. The fish were then examined using quantitative RT‐PCR by comparing the messenger RNA level of reproduction‐related genes (gonadotropin releasing hormone 1; gnrh1, gonadotropin releasing hormone 2; gnrh2, follicle stimulating hormone; fshβ, luteinizing hormone; lhβ and DA receptor 2b; d2b), involved in the gonadotropic axis in eels, to those that received a control injection. The results indicate significant differences in the expression levels of gnrh1, gnrh2 and d2b in the brain and d2b, fshβ, lhβ in the pituitary at different stages of sexual maturation. Melatonin appears to enhance the production of sex gonadotropins, whereas DA inhibits them. These findings suggest an interaction between melatonin and DA in regulating reproduction in Japanese eels.

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Molecular and physiological study of the artificial maturation process in European eel males: From brain to testis

Cited by 51 publications

References 84 publications

Induced maturation of eel weighed 100–150 gram with PMSG, antidopamine, and 17α-methyltestosterone

Induced maturation of eel weighed 100–150 gram with PMSG, antidopamine, and 17α-methyltestosterone

Molecular approaches related to the European eel (Anguilla anguilla) reproductive process

Effects of dopamine and melatonin treatment on the expression of the genes associated with artificially induced sexual maturation in Japanese eel, Anguilla japonica

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