Molecular and Phenotypic Characterization of Colletotrichum Species Associated with Anthracnose Disease of Papaya in Trinidad

Abstract

Anthracnose disease is a major limiting factor to papaya production worldwide. Accurate identification of the pathogens responsible for this disease is important to developing disease management strategies. One hundred and three (103) isolates of Colletotrichum were collected from infected papaya fruits cvs. Red lady and Tainung No. 2 - F1 hybrid in Trinidad. Of all isolates, 79% were C. gloeosporioides and 21% were C. truncatum. Spore morphology, cultural characteristics, differential reaction to benomyl in addition to ITS1 and β-tubulin gene sequence comparisons unequivocally identified and separated the two species of Colletotrichum. Certain characteristics enabled discrimination between the two species and may be used for provisional identification of these species isolated from papaya. Isolates of C. gloeosporioides grew at a significantly faster rate than those of C. truncatum. C. gloeosporioides isolates were sensitive to benomyl at 1.0 μg/ml, but C. truncatum isolates were resistant. Pathogenicity tests revealed that Colletotrichum species and papaya cultivar had no significant effect on lesion diameter. In cross-infection studies, isolates of C. gloeosporioides from mango and C. truncatum from sweet pepper were able to infect and cause symptoms in wounded mature papaya fruit under controlled conditions. There was no evidence of anthracnose infection in seeds of infected fruits based on the results of growing-on tests. Phylogenetic analyses were based on comparisons of ITS1 and β-tubulin gene sequences. Both neighbor-joining and maximum parsimony methods resolved all Colletotrichum isolates from papaya into species-specific clusters with high bootstrap support. Additionally, a pair of species-specific primers were developed (Ct-TUB-F/R) which allowed reliable detection of C. truncatum isolates from papaya.

Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia

“…The analysis also showed that location, banana cultivar and morphotype did not contribute to phylogenetic relatedness. The results were similar to those of a study by Rampersad (2011) on papaya anthracnose in which geographical distribution, host cultivar or morphotype was not an indication of the phylogenetic relatedness of the papaya isolates. C. gloeosporioides and C. musae are part of the taxa in C. gloeosporioides species complex in which the species in this complex has broad genetic and biological diversity (Damm et al, 2010).…”

“…The findings of this study have an important bearing on the epidemiology of anthracnose disease since different species of Colletotrichum may have different conidial dispersal capacities (Ntahimpera et al, 1999;Rampersad, 2011). Accurate identification of the anthracnose-causing pathogen is also important, since different species of Colletotrichum may vary in their reaction to different types of fungicides (Rampersad, 2011).…”

“…Accurate identification of the anthracnose-causing pathogen is also important, since different species of Colletotrichum may vary in their reaction to different types of fungicides (Rampersad, 2011). Sequence analysis using other protein-coding genes should be tested to confirm the existence of more than one distinct species of C. gloeosporioides, since this species has wide geographic distribution and host range.…”

Molecular Characterization and Phylogenetic Analysis of Colletotrichum Species Associated with Anthracnose Disease

“…Fruit production is mostly affected in both high-value crops and wild fruits in natural habitats. However, Colletotrichum species associated with wild fruits are poorly known [4,5]. Colletotrichum specie was voted as the eighth most important plant pathogens in the world in a recent survey among fungal pathologists, for its perceived scientific and economic importance [6].…”

Substâncias GRAS no controle do crescimento de Colletotrichum gloeosporioides e Fusarium guttiforme in vitro

“…As espécies pertencentes ao gênero, geralmente são responsáveis pela antracnose e outras doenças em uma grande variedade de culturas incluindo cereais, leguminosas, olerícolas e frutos [3][4][5][6].…”