Molecular and pharmacological characterization of a β-adrenergic-like octopamine receptor from the green rice leafhopper Nephotettix cincticeps

Xu, Gang; Chang, Xuefei; Gu, Gui-Xiang; Jia, Wenxi; Guo, Lei; Huang, Jia; Yè, Gōngyín

doi:10.1016/j.ibmb.2020.103337

Cited by 9 publications

(14 citation statements)

References 75 publications

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“…5). This finding agrees with the general perception of BARs as being preferentially expressed in the neurons of the central nervous system and is consistent with previous studies in insects 89–93 . Most genes showed significantly high expression in the hemolymph tissues (Fig.…”

Section: Discussionsupporting

confidence: 93%

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Liu

Zhan

et al. 2021

Pest Management Science

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BACKGROUD Insect biogenic amines play important roles in mediating behavioral and physiological processes. They exert their effects by binding to biogenic amine receptors (BARs), which are specific receptor proteins in the G‐protein‐coupled receptor superfamily. BAR genes have been cloned and characterized from multiple model insects, including Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Apis mellifera and Tribolium castaneum. However, relatively little work has addressed the molecular properties, expression profiles, and pharmacological characterization of BARs from other insects, including important pests. RESULTS In this study, we cloned 17 genes encoding putative biogenic amine receptor proteins from Plutella xylostella, a global pest of Brassica crops. These PxBAR genes were five octopamine receptors (PxOA1, PxOA2B1, PxOA2B2, PxOA2B3, and PxOA3), three tyramine receptors (PxTAR1A, PxTAR1B, and PxTAR2), four dopamine receptors (PxDOP1, PxDOP2, PxDOP3, and PxDopEcR), and five serotonin receptors (Px5‐HT1A, Px5‐HT1B, Px5‐HT2A, Px5‐HT2B, and Px5‐HT7). All PxBARs showed considerable sequence identity with orthologous BARs, and phylogenetic analysis clustered the receptors within their respective groups while preserving organismal evolutionary relationships. We investigated their molecular properties and expression profiles, and pharmacologically characterized the dopamine receptor, PxDOP2. CONCLUSIONS Our study provides important information and resources on biogenic amine receptors from P. xylostella, which suggests potential target sites for controlling this pest species. © 2021 Society of Chemical Industry.

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Section: Discussionsupporting

confidence: 93%

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Liu

Zhan

et al. 2021

Pest Management Science

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“…CcOctβ3R was predominantly expressed in the antenna, and the CcOctβ2R transcript in the antenna was second only to that in the head. BmOctβ2R [ 35 ] and NcOctβ2R [ 70 ] were also expressed in the antenna. Interestingly, octopaminergic modulation in the antenna of honeybee workers could be enhanced not only by increasing the OA level, but also by upregulation of OARs [ 71 ], and OA has been reported to increase the olfactory responses of Heliothis virescens to female sex pheromones [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

“…CcOctβRs were all expressed in the leg. The transcript levels of NcOctβ2R [ 70 ] and TcOctβ2R [ 50 ] were found to exhibit high expressions in the leg. There are large muscles in insect legs, and the expressions of DmOctβRs were all detected in the adult muscles [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular and Pharmacological Characterization of β-Adrenergic-like Octopamine Receptors in the Endoparasitoid Cotesia chilonis (Hymenoptera: Braconidae)

Zhang

et al. 2022

IJMS

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Octopamine (OA) is structurally and functionally similar to adrenaline/noradrenaline in vertebrates, and OA modulates diverse physiological and behavioral processes in invertebrates. OA exerts its actions by binding to specific octopamine receptors (OARs). Functional and pharmacological characterization of OARs have been investigated in several insects. However, the literature on OARs is scarce for parasitoids. Here we cloned three β-adrenergic-like OARs (CcOctβRs) from Cotesia chilonis. CcOctβRs share high similarity with their own orthologous receptors. The transcript levels of CcOctβRs were varied in different tissues. When heterologously expressed in CHO-K1 cells, CcOctβRs induced cAMP production, and were dose-dependently activated by OA, TA and putative octopaminergic agonists. Their activities were inhibited by potential antagonists and were most efficiently blocked by epinastine. Our study offers important information about the molecular and pharmacological properties of β-adrenergic-like OARs from C. chilonis that will provide the basis to reveal the contribution of individual receptors to the physiological processes and behaviors in parasitoids.

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“…As a potential insecticide target, OctβRs have been studied for their pharmacological characterizations. The interaction of OctβRs on agonists and antagonists was tested in Plutella xylostella [ 26 ], B. mori [ 27 ], N. lugens [ 18 ], Rhodnius prolixus [ 24 ], and Nephotettix cincticeps [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Knockdown of a β-Adrenergic-Like Octopamine Receptor Affects Locomotion and Reproduction of Tribolium castaneum

Zheng

Liu

et al. 2021

IJMS

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The neurohormone octopamine regulates many crucial physiological processes in insects and exerts its activity via typical G-protein coupled receptors. The roles of octopamine receptors in regulating behavior and physiology in Coleoptera (beetles) need better understanding. We used the red flour beetle, Tribolium castaneum, as a model species to study the contribution of the octopamine receptor to behavior and physiology. We cloned the cDNA of a β-adrenergic-like octopamine receptor (TcOctβ2R). This was heterologously expressed in human embryonic kidney (HEK) 293 cells and was demonstrated to be functional using an in vitro cyclic AMP assay. In an RNAi assay, injection of dsRNA demonstrated that TcOctβ2R modulates beetle locomotion, mating duration, and fertility. These data present some roles of the octopaminergic signaling system in T. castaneum. Our findings will also help to elucidate the potential functions of individual octopamine receptors in other insects.

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Molecular and pharmacological characterization of a β-adrenergic-like octopamine receptor from the green rice leafhopper Nephotettix cincticeps

Cited by 9 publications

References 75 publications

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Molecular and pharmacological characterization of biogenic amine receptors from the diamondback moth, Plutella xylostella

Molecular and Pharmacological Characterization of β-Adrenergic-like Octopamine Receptors in the Endoparasitoid Cotesia chilonis (Hymenoptera: Braconidae)

Knockdown of a β-Adrenergic-Like Octopamine Receptor Affects Locomotion and Reproduction of Tribolium castaneum

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