MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

Awad, Hassan M.; Germoush, Mousa O.

doi:10.22159/ajpcr.2017.v10i10.20201

Cited by 4 publications

(3 citation statements)

References 28 publications

(40 reference statements)

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“…In this case, the taxonomy of Streptomyces dependent on traditional methods is insufficient most of the time. Consequently, genotypic methods, dominatingly rRNA genetic material structures, have been presented [22]. It very well may be utilized as a phylogenetic strategy corresponding to the traditional methods [34].…”

Section: Discussionmentioning

confidence: 99%

“…The 16S rDNA was amplified by polymerase chain reaction (PCR) using primers designed to amplify a 1500bp fragment of the 16S rDNA region. The domain bacteria-specific primer 27F (forward primer) was 5′ AGAGTTTGATCMTGGCTCAG3′, and the universal bacterial primer 1492R (reverse primer) was 5′TACG-GYTACCTTGTTACGACTT3′ [20][21][22].…”

Section: Phylogenetic Identificationmentioning

confidence: 99%

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New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

Mohamed

Awad

2021

Beni-Suef Univ J Basic Appl Sci

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Background Bioprospecting lipase producers in non-conventional habitats are the way to find special enzymes of diverse applications. Halo-alkaline marshes in Wadi El Natrun in Egypt are some of the most stable ecological systems in the world, and because of the double extremities of alkalinity and salinity, they harbor individual microbes capable of adapting stress conditions. Results Eight strains were recovered from the coastline soil of Al-Beida Lake in Wadi El Natrun and have been tested for lipase production. Among the eight isolates, the strain SBLWN_MH2 was the most active producer of lipase (7.5 U/ml). The crude SBLWN_MH2 lipase showed activity over a wide pH range (3.5 to 13) with an optimum pH at 10.5, and it was able to show more than 75% of its highest activity at pH elevated up to 13. The identification using phenotypic and genotypic methods strongly indicated that the strain SBLWN_MH2 belonged to the genus Streptomyces with a similarity of 99%. Thus, it has been given the suggested name Streptomyces sp. SBLWN_MH2 (MG593538). SBLWN_MH2 produced extracellular lipase in modified starch casein medium supplemented with different oils or Tween-80, and the potential production rate has been attained in the case of linseed oil after 3 days. Further experiments have been carried out to optimize medium composition through Box-Behnken design and response surface methodology, and it was possible to achieve more than 3.5-fold increase in lipase production. Conclusions The present study indicates that Streptomyces sp. SBLWN_MH2 is a potential lipase producer and could be fruitfully employed in the large-scale production of highly alkaline lipase.

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Section: Discussionmentioning

confidence: 99%

Section: Phylogenetic Identificationmentioning

confidence: 99%

New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

Mohamed

Awad

2021

Beni-Suef Univ J Basic Appl Sci

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“…Separately-sterilized carbon sources were added to a final concentration of 1.0%. The plates were incubated at 30C and the growth using glucose as positive control was noticed after 7, 14 and 21 days (Ismail et al, 2017;Awad & Germoush, 2017).…”

Section: Article Infomentioning

confidence: 99%

Isolation and Identification of Cholesterol Oxidase Producing Actinomycetes From Egyptian Soil for a Large Scale Fermentation in Submerged Cultures

Abdelwahed¹

2021

J microb biotech food sci

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Isolation and identification of new microbial isolates capable of producing cholesterol oxidase enzyme (CHO) is of great potential in the field of biotechnology of industrial enzymes. Four producing strains of actinomycetes were isolated from Egyptian soils demonstrated high activity in cholesterol degradation compared to the other isolates. Based on different standard morphological, biochemical as well as molecular techniques using 16S rRNA sequencing, these actinomycetes were identified as Streptomyces sp. strain NHIA_CH3 MK680299, Streptomyces sp. strain NHIA_CH5 MK680300 , Streptomyces griseus strain NHIA_CH6 MK680301 and Streptomyces drozdowiczii strain NHIA_CH10 MK680302. In order to optimize the CHO enzyme activity, different fermentation media were tested in submerged cultures at shake flask level. Maximum activity (23.7 U/mL) was observed in culture of Streptomyces sp. strain NHIA_CH5 which was scaled up to the 5L volume of the stirred tank bioreactor. Results of the batch fermentation showed an earlier production of CHO enzyme with 4% increase in activity compared to that obtained from shake flask cultures indicated that Streptomyces sp. strain NHIA_CH5 could be a potential source of CHO enzyme for commercial purpose.

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Optimization of the Cytotoxic Activity of Three Streptomyces Strains Isolated from Guaviare River Sediments (Colombia, South America)

Ramírez-Rodríguez

Stepanian-Martinez

Morales-González

et al. 2018

BioMed Research International

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Three Streptomyces strains isolated from Guaviare sediments (Colombia, South America) with cytotoxic activity against prostate cancer (PC3), breast cancer (MDA-MB-231), and lung cancer (A549) line cells were studied. The present investigation reveals the enhancement of the cytotoxic activity evaluating different values of pH, carbon sources (sucrose, glucose, xylose, maltose, and starch), and nitrogen sources (malt extract, yeast extract, meat extract, peptone, and potassium nitrate). Based on the response surface methodology, the isolates Streptomyces aburaviensis (73) had the maximum activity for lung cancer (IC50= 25.00 ± 1.86 ppm) with 4% of yeast extract, 3% of starch, and a pH value of 7. Streptomyces gramineus (386) had the maximum activity against prostate cancer (IC50= 6.14 ± 2.07 ppm) with 5% of malt extract, 3% of glucose, and a pH value of 6. Finally, Streptomyces psammoticus (519) had the maximum activity against breast cancer (IC50= 35.53 ± 2.71 ppm) with 1% of yeast extract, 4% of starch, and a pH 8. The results suggest that the ethyl acetate extracts from isolates Streptomyces aburaviensis (73), Streptomyces gramineus (386), and Streptomyces psammoticus (519) have a potential for use in pharmaceuticals as cytotoxic agents.

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MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

Cited by 4 publications

References 28 publications

New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

Isolation and Identification of Cholesterol Oxidase Producing Actinomycetes From Egyptian Soil for a Large Scale Fermentation in Submerged Cultures

Optimization of the Cytotoxic Activity of Three Streptomyces Strains Isolated from Guaviare River Sediments (Colombia, South America)

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