Optimization of the Cytotoxic Activity of Three Streptomyces Strains Isolated from Guaviare River Sediments (Colombia, South America)

Abstract

Three Streptomyces strains isolated from Guaviare sediments (Colombia, South America) with cytotoxic activity against prostate cancer (PC3), breast cancer (MDA-MB-231), and lung cancer (A549) line cells were studied. The present investigation reveals the enhancement of the cytotoxic activity evaluating different values of pH, carbon sources (sucrose, glucose, xylose, maltose, and starch), and nitrogen sources (malt extract, yeast extract, meat extract, peptone, and potassium nitrate). Based on the response surface methodology, the isolates Streptomyces aburaviensis (73) had the maximum activity for lung cancer (IC50= 25.00 ± 1.86 ppm) with 4% of yeast extract, 3% of starch, and a pH value of 7. Streptomyces gramineus (386) had the maximum activity against prostate cancer (IC50= 6.14 ± 2.07 ppm) with 5% of malt extract, 3% of glucose, and a pH value of 6. Finally, Streptomyces psammoticus (519) had the maximum activity against breast cancer (IC50= 35.53 ± 2.71 ppm) with 1% of yeast extract, 4% of starch, and a pH 8. The results suggest that the ethyl acetate extracts from isolates Streptomyces aburaviensis (73), Streptomyces gramineus (386), and Streptomyces psammoticus (519) have a potential for use in pharmaceuticals as cytotoxic agents.

Isolation and Identification of Actinomycetes Associated with Moss on the Surface of the Borobudur Temple Stone

“…Micromonospora maritima D10-9-5 100.00 Some species in this study have been reported successful isolated from various samples such as soil, plant, pollutant water, coastal, sea sediment, or human body (Carro et al, 2018;Luo, Hu, Peng, Zhang, & Wang, 2015;Wibberg et al, 2018;Klymyshin et al, 2013;Bourbour, Keikha, & Faghri, 2018;Tsang et al, 2016;Chen, Tang, Mori, & Wu, 2012;Kumar et al, 2016;Xie et al, 2016a). The isolates have been reported as a source of several secondary metabolites were Micromonospora halophytica (Carro et al, 2018), Microbacterium ginsengisoli (Palaniappan et al, 2010), Streptomyces avellaneus (Ramirez-Rodriguez et al, 2018), Streptomyces bungoensis (Michael et al, 2017), Streptomyces chartreusis (Wibberg et al, 2018), Streptomyces echinatus (Klymyshin et al, 2013), Streptomyces griseoluteus (Luo et al, 2015), Streptomyces griseoruber (Wang, Peng, Zhang, Hui, & Hu, 2014), Streptomyces neopeptinius (Goudjal et al, 2016), Streptomyces polychromogenes (Mitsukura, Sakamoto, Kubo, Yoshida, & Nagasawa, 2010) , Streptomyces termitum (Sales et al, 2017), and Streptomyces violaceorectus (Fallah, 2018).…”

Actinomycins from Soil-Inhabiting Streptomyces as Sources of Antibacterial Pigments for Silk Dyeing

“…Our results indicate that the crude extract from Streptomyces gramineus TBRC 15927 was not toxic to the L-929 mouse fibroblast cells, with an IC 50 value of 0.029 ± 0.008 µg/mL (0.029 ppm). According to the report by Ramirez-Rodriguez et al [ 56 ], the toxicity of the L-929 mouse fibroblast cells was tested with Doxorubicin (1–25 ppm) as a standard substance. They concluded that crude extracts with an IC 50 value of less than 50 ppm are not significantly toxic to mammal cells.…”

Bacterial Pigments and Their Multifaceted Roles in Contemporary Biotechnology and Pharmacological Applications

“…Numerous species of the genus Streptomyces are known to produce many bioactive compounds and pigments that have demonstrated anti-tumour activity. In the search for a novel anticancer medication, S. aburaviensis and S. psammoticus are preferred possibilities [ 64 ]. S. gresioaurianticus JUACT 01 produces a yellow-coloured pigment that induces apoptosis in HeLa and HepG2 cell lines and is nontoxic to the human lymphocytes and is also a promising candidate for further investigations [ 65 ].…”