Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi

Carrillo, Carolina; Canepa, Gaspar E.; Algranati, Israel D.; Pereira, Claudio A.

doi:10.1016/j.bbrc.2006.03.215

Cited by 51 publications

(39 citation statements)

References 21 publications

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“…Mulangi et al ( 2012b ) searched for rice and Arabidopsis genes that show sequence similarity to PA transporters of Leishmania major ( LmPOT1 ) (Hasne and Ullman 2005 ) and Trypanosoma cruzi ( TcPAT12 ) (Carrillo et al 2006 ). Full-length cDNAs of candidate genes were transformed into the Saccharomyces cerevisiae agp2∆ mutant, which is defi cient in Spd transport (Aouida et al 2005 ).…”

Section: Identifi Cation Of Polyamine Transporter Genes Using Yeast Cmentioning

confidence: 99%

Polyamine Transport Systems in Plants

Fujita

Shinozaki

2014

Polyamines

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Polyamine (PA) transport has been analyzed at the cell and organ levels in several plant species, but currently the molecular mechanisms of PA transport are not completely understood. Several papers recently identifi ed plant PA transporters. A study of Arabidopsis oxidative stress responses to the herbicide paraquat (PQ) identifi ed a LAT (L-type amino acid transporter) family protein as a transporter of both PQ and PA. Other studies based on yeast complementation analyses revealed that LAT family genes in rice and Arabidopsis function as PA transporters. Arabidopsis LAT proteins exhibited different subcellular localizations, suggesting that these transporters could be involved in both intracellular PA traffi cking and PA uptake across the plasma membrane. These results provide novel insights into plant PA transport and explain the mechanism for PA-mediated protection against PQ toxicity. In this review, we focus on recent advances in our understanding of plant PA transport systems and discuss the roles of the recently identifi ed plant PA transporters.

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Section: Identifi Cation Of Polyamine Transporter Genes Using Yeast Cmentioning

confidence: 99%

Polyamine Transport Systems in Plants

Fujita

Shinozaki

2014

Polyamines

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“…LmPOT1 from L. major was characterized as a putrescine preferential transporter by heterologous expression in Xenopus laevis oocytes (Hasne and Uhlman 2005). A similar strategy was used to characterize TcPAT12 from T. cruzi, the etiologic agent of Chagas disease (Carrillo et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of OsPUT1, a rice polyamine uptake transporter

Mulangi

Phuntumart

Aouida

et al. 2011

Planta

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Polyamines are nitrogenous compounds found in all eukaryotic and prokaryotic cells and absolutely essential for cell viability. In plants, they regulate several growth and developmental processes and the levels of polyamines are also correlated with the plant responses to various biotic and abiotic stresses. In plant cells, polyamines are synthesized in plastids and cytosol. This biosynthetic compartmentation indicates that the specific transporters are essential to transport polyamines between the cellular compartments. In the present study, a phylogenetic analysis was used to identify candidate polyamine transporters in rice. A full-length cDNA rice clone AK068055 was heterologously expressed in the Saccharomyces cerevisiae spermidine uptake mutant, agp2∆. Radiological uptake and competitive inhibition studies with putrescine indicated that rice gene encodes a protein that functioned as a spermidine-preferential transporter. In competition experiments with several amino acids at 25-fold higher levels than spermidine, only methionine, asparagine, and glutamine were effective in reducing uptake of spermidine to 60% of control rates. Based on those observations, this rice gene was named polyamine uptake transporter 1 (OsPUT1). Tissue-specific expression of OsPUT1 by semiquantitative RT-PCR showed that the gene was expressed in all tissues except seeds and roots. Transient expression assays in onion epidermal cells and rice protoplasts failed to localize to a cellular compartment. The characterization of the first plant polyamine transporter sets the stage for a systems approach that can be used to build a model to fully define how the biosynthesis, degradation, and transport of polyamines in plants mediate developmental and biotic responses.

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“…The transporters involved in diamine uptake have been 275 functionally characterized in epimastigotes and they contain thiol 276 residues that are critical for their activity [60]. A gene coding for a 277 spermidine transporter has been expressed in an heterologous system 278 where it was shown to be functional [61]. However, characterization 279 of this transporter in T. cruzi is still lacking.…”

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confidence: 99%