Molecular and epidemiological characterization of canine<i>Pseudomonas</i>otitis using a prospective case‐control study design

Morris, Daniel O.; Davis, Meghan F.; Palmeiro, B.; O’Shea, Kathleen; Rankin, Shelley C.

doi:10.1111/vde.12347

Cited by 12 publications

(11 citation statements)

References 30 publications

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“…Larger studies would be required to look at the relationship between breed characteristics and OE. Prior antimicrobial or anti‐inflammatory therapy may impact the microbiota of these individuals as well, although the impact could not be assessed in the present study due to varied therapeutics used, varied chronicity and the sample size. Concurrent and underlying dermatological disease was not a focus of this study.…”

Section: Discussionmentioning

confidence: 99%

The otic microbiota and mycobiota in a referral population of dogs in eastern USA with otitis externa

Bradley

Lee

Rankin

et al. 2020

Veterinary Dermatology

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Background -Canine otitis externa (OE) is a common inflammatory disease that is frequently complicated by secondary bacterial and/or yeast infections. The otic microbial population is more complex than appreciated by cytological methods and aerobic culture alone.Hypothesis/objectives -Differences in bacterial and fungal populations of the external ear canal will correlate with specific cytological and culture-based definitions of bacterial and Malassezia otitis.Animals -Forty client-owned dogs; 30 with OE and 10 with healthy ears.Methods and materials -Prospective study comparing cytological samples, aerobic bacterial cultures and culture-independent sequencing-based analyses of the external ear canal. Subjects with OE included 10 dogs with only cocci [≥25/high power field (HPF)] on cytological evaluation and culture of Staphylococcus spp.; 10 dogs with rods (≥25/HPF) and exclusive culture of Pseudomonas aeruginosa; 10 dogs with only yeast on cytological results morphologically compatible with Malassezia spp. (≥5/HPF).Results -Staphylococcus was the most abundant taxa across all groups. Ears cytologically positive for cocci had decreased diversity, and all types of OE were associated with decreased fungal diversity compared to controls.Conclusions and clinical importance -Cytological and culture-based assessment of the ear canal is not predictive of the diverse microbiota of the ear canal in cases of Pseudomonas or Malassezia otitis. Less abundant bacterial taxa in cases of staphylococcal OE are worth scrutiny for future biological therapy.

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Section: Discussionmentioning

confidence: 99%

The otic microbiota and mycobiota in a referral population of dogs in eastern USA with otitis externa

Bradley

Lee

Rankin

et al. 2020

Veterinary Dermatology

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“…Owing to the chronic and treatment‐resistant nature of P. aeruginosa infections in dogs, long‐term antimicrobial treatment often is required . However, few studies have assessed antimicrobial resistance in clinical P. aeruginosa isolates from dogs and previous reports have found that the incidence of carbapenem‐nonsusceptible P. aeruginosa isolated from dogs was low . There is a report on carbapenemase‐producing P. aeruginosa from an anal swab of a healthy dog in China, which was highly similar to that from humans in the same area of China .…”

Section: Introductionmentioning

confidence: 99%

Identification of VIM‐2 metallo‐β‐lactamase‐producing Pseudomonas aeruginosa isolated from dogs with pyoderma and otitis in Korea

Hyun

Chung

Hwang

2018

Veterinary Dermatology

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“…This could also explain the seasonal pattern that was found in the present study, regarding topical AMU since TAMs are applied in dermatological conditions and in cases of otitis [ 2 , 31 ]. Furthermore, in warmer months, owners and their pets might spend more time outdoors engaging in activities such as swimming in open water, which might predispose dogs for ear infections [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Quantifying topical antimicrobial use before and during participation in an antimicrobial stewardship programme in Dutch companion animal clinics

et al. 2023

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The emergence of bacterial strains resistant to topical antimicrobials in both human and veterinary medicine has raised concerns over retaining the efficacy of these preparations. Yet, little information is available regarding the use of topical antimicrobials in either sector for planning targeted interventions. This study aims to quantify the use of topical antimicrobials in 44 Dutch companion animal clinics before and during their participation in an antimicrobial stewardship programme (ASP), to explore the effect of the intervention on topical antimicrobial use (AMU). Hence, prescription and clinic animal population data, collected from July 2012 until June 2018 were used. Specifically, the period from July 2012 until June 2015 was defined as pre-intervention period, whereas clinics started to participate in the ASP from March 2016 onwards. As quantification metric, the Defined Daily Dose for Animals (DDDA) was used and a mixed effect times series model with auto-regression was applied to monthly topical AMU data. The intervention effect was modelled using a step function with a change in (linear) time trend and clinic characteristics, as potential determinants of topical AMU, were assessed using a multivariable regression model. A seasonal pattern was identified, in the pre-intervention period, where topical AMU was highest in July-August and lowest in February-March. In addition, total topical AMU appeared to significantly decrease over time in the pre-intervention period and the proportion of dogs in the clinic was positively associated with topical AMU. The intervention effect was significant only for second line and for skin product AMU. This study demonstrates that during participation in an ASP, second line and skin product AMU decreased in Dutch companion animal clinics. Additionally, this study demonstrates the existence of a seasonal effect and a decrease in topical AMU over time already before introduction of a targeted intervention.

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Molecular and epidemiological characterization of caninePseudomonasotitis using a prospective case‐control study design

Abstract: Genetic homology between otic and environmental isolates is consistent with a waterborne source for some dogs, and cross-contamination with other human and animal members within some households.

Cited by 12 publications

References 30 publications

The otic microbiota and mycobiota in a referral population of dogs in eastern USA with otitis externa

The otic microbiota and mycobiota in a referral population of dogs in eastern USA with otitis externa

Identification of VIM‐2 metallo‐β‐lactamase‐producing Pseudomonas aeruginosa isolated from dogs with pyoderma and otitis in Korea

Quantifying topical antimicrobial use before and during participation in an antimicrobial stewardship programme in Dutch companion animal clinics

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