Identification of <scp>VIM</scp>‐2 metallo‐β‐lactamase‐producing <i>Pseudomonas aeruginosa</i> isolated from dogs with pyoderma and otitis in Korea

Hyun, Jae‐Eun; Chung, Tae‐Ho; Hwang, Cheol‐Yong

doi:10.1111/vde.12534

Cited by 19 publications

(28 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antimicrobial profiles of canine P. aeruginosa isolates are difficult to compare between different studies, due to many variables that represent a challenge for the harmonization of global data. In particular, antimicrobial methods used [ 33 ], interpretative criteria applied [ 34 ], body sites of sampling [ 35 ], geographical [ 36 ], and temporal differences [ 37 ] represent all variables that have to be taken into account. In the present study, the MICs values were determined by means of diffusion gradient method on agar, using a predefined antibiotic gradient strip.…”

Section: Discussionmentioning

confidence: 99%

Occurrence, antimicrobial susceptibility, and pathogenic factors of Pseudomonas aeruginosa in canine clinical samples

et al. 2021

View full text Add to dashboard Cite

Background and Aim: Pseudomonas aeruginosa is a relevant opportunistic and difficult to treat pathogen due to its widespread environmental diffusion, intrinsic resistance to many classes of antimicrobials, high ability to acquire additional resistance mechanisms, and wide range of pathogenic factors. The present study aimed to investigate the prevalence of P. aeruginosa in canine clinical samples, the antimicrobial susceptibility against antipseudomonal antibiotics, and the presence of extracellular pathogenic factors of the isolates, as well as their ability to produce biofilm. Materials and Methods: Overall, 300 clinical specimens from dogs with pyoderma or abscesses (n=58), otitis (n=59), and suspected bladder infection (n=183) were analyzed by standard bacteriological methods. P. aeruginosa isolates were tested for their antimicrobial susceptibility by disk and gradient diffusion methods to determine the minimum inhibitory concentrations. The ability of the isolates to produce biofilm was investigated by a microtiter plate assay, while virulence genes coding for elastase (lasB), exotoxin A (toxA), alkaline protease (aprA), hemolytic phospholipase C (plcH), and exoenzyme S (ExoS) were detected by polymerase chain reaction method. Results: A total of 24 isolates of P. aeruginosa were found in clinical specimens (urine n=3, skin/soft tissue n=6, and ear canal n=15). No resistance was found to ceftazidime, gentamicin, aztreonam, and imipenem (IMI), while low levels of resistance were found to enrofloxacin (ENR) (4.2%) and piperacillin-tazobactam (8.3%). However, 41.7% and 29.2% of the isolates showed intermediate susceptibility to ENR and IMI, respectively. Disk and gradient diffusion methods showed high concordance. The majority of the isolates revealed a weak (33.3%) or intermediate (45.8%) ability to form biofilm, while the strong biofilm producers (20.8%) derived exclusively from the ear canal samples. All isolates (100%) were positive for lasB, aprA, and plcH genes, while exoS and toxA were amplified in 21 (87.5%) and 22 (91.7%) isolates, respectively. Conclusion: In the present study, P. aeruginosa isolates from canine clinical samples were characterized by low levels of antimicrobial resistance against antipseudomonal drugs. However, the high presence of isolates with intermediate susceptibility for some categories of antibiotics, including carbapenems which are not authorized for veterinary use, could represent an early warning signal. Moreover, the presence of isolates with strong ability to produce biofilm represents a challenge for the interpretation of the antimicrobial susceptibility profile. In addition, the high prevalence of the extracellular pathogenic factors was indicative of the potential virulence of the isolates.

show abstract

Section: Discussionmentioning

confidence: 99%

Occurrence, antimicrobial susceptibility, and pathogenic factors of Pseudomonas aeruginosa in canine clinical samples

et al. 2021

View full text Add to dashboard Cite

show abstract

“…has been reported worldwide in dogs. [7][8][9] The increasing resistance to antimicrobial agents among bacterial pathogens has led to a need for novel therapeutic agents or more effective treatment methods.…”

Section: Introductionmentioning

confidence: 99%

In vitro antibacterial activity of the manuka essential oil from Leptospermum scoparium combined with Tris‐EDTA against Gram‐negative bacterial isolates from dogs with otitis externa

Song

Hyun

Kang

et al. 2019

Veterinary Dermatology

Self Cite

View full text Add to dashboard Cite

Background The increasing prevalence of antimicrobial resistance among bacteria in dogs with otitis externa has led to a need for novel therapeutic agents. Hypothesis/Objective To examine the antibacterial effects of manuka oil combined with ethylenediaminetetraacetic acid–tromethamine (Tris‐EDTA) against Gram‐negative bacteria isolates from dogs with otitis externa. Methods and materials A total of 53 clinical isolates including Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae ssp. pneumoniae and Proteus mirabilis. Antimicrobial susceptibility was determined using disk diffusion; the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of manuka essential oil, with or without Tris‐EDTA, were investigated. Results A total of 44 isolates were resistant to at least one antibiotic and 19 strains were multidrug‐resistant, with resistance to at least one agent in three or more antimicrobial classes. The MICs and MBCs of manuka oil alone were ≥1% (v/v) and ≥2% (v/v), respectively. There was no antimicrobial effect of Tris‐EDTA (1.125:0.3 mg/mL) without manuka oil. However, the combination of manuka oil with Tris‐EDTA significantly decreased the MICs (ranging from 0.06% to 0.5%, v/v; P < 0.001) and MBCs (ranging from 0.06% to 1%, v/v; P < 0.001). There also was no significant difference between multidrug‐resistant and nonresistant bacterial isolates in terms of the antimicrobial activity of manuka oil with Tris‐EDTA. Conclusions and clinical importance The study findings suggest that manuka oil, especially when combined with Tris‐EDTA, may be a promising alternative therapeutic option for Gram‐negative otic pathogens. Clinical studies are needed to assess potential for in vivo ototoxic effects and efficacy.

show abstract

“…This isolate, identified in Norway in 2010, was found to produce VIM-type MBLs. Recently, VIM-2-producing P. aeruginosa ST1047 isolates were obtained from dogs in South Korea with pyoderma and otitis (17). P. aeruginosa high-risk clones, including ST233, ST235, and ST357, were also identified in Myanmar but from only 26.7% of the 45 isolates.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Multidrug-Resistant Pseudomonas aeruginosa Isolates in Hospitals in Myanmar

Tada

Hishinuma

Watanabe

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa has become a serious worldwide medical problem. This study was designed to clarify the genetic and epidemiological properties of MDR P. aeruginosa strains isolated from hospitals in Myanmar. Forty-five MDR P. aeruginosa isolates obtained from different patients in seven hospitals in Myanmar were screened using the broth microdilution method. The whole genomes of the MDR isolates were sequenced using a MiSeq platform (Illumina). Phylogenetic trees were constructed from single nucleotide polymorphism concatemers. Multilocus sequence types were deduced, and drug resistance genes were identified. Of the 45 isolates, 38 harbored genes encoding carbapenemases, including DIM-1, IMP-1, NDM-1, VIM-2, and VIM-5, and 9 isolates had genes encoding 16S rRNA methylases, including RmtB, RmtD3, RmtE, and RmtF2. Most MDR P. aeruginosa strains isolated in Myanmar belonged to sequence type 1047 (ST1047). This is the first molecular epidemiological analysis of MDR P. aeruginosa clinical isolates in Myanmar. These findings strongly suggest that P. aeruginosa ST1047 strains harboring carbapenemases, including DIM-, IMP-, NDM-, and VIM-type metallo-␤lactamases, have been spreading throughout medical settings in Myanmar.

show abstract

Identification of VIM‐2 metallo‐β‐lactamase‐producing Pseudomonas aeruginosa isolated from dogs with pyoderma and otitis in Korea

Abstract: This study demonstrated the diversity of the phenotype and genotype of clinical P. aeruginosa isolates from dogs with pyoderma and otitis. The identification of VIM-2-producing P. aeruginosa in dogs is alarming and warrants further surveillance.

Cited by 19 publications

References 40 publications

Occurrence, antimicrobial susceptibility, and pathogenic factors of Pseudomonas aeruginosa in canine clinical samples

Occurrence, antimicrobial susceptibility, and pathogenic factors of Pseudomonas aeruginosa in canine clinical samples

In vitro antibacterial activity of the manuka essential oil from Leptospermum scoparium combined with Tris‐EDTA against Gram‐negative bacterial isolates from dogs with otitis externa

Molecular Characterization of Multidrug-Resistant Pseudomonas aeruginosa Isolates in Hospitals in Myanmar

Contact Info

Product

Resources

About