Molecular and Conventional Identification and Pathogenicity of <i>Rhizoctonia solani</i> Isolates from Tobacco (<i>Nicotiana tabacum</i> L.) in Samsun, Turkey

Gürkanlı, Cem Tolga; Özkoç, İbrahim; Gündüz, İslam

doi:10.1111/j.1439-0434.2009.01564.x

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…In the pathogenicity tests, R. solani AG 2-1 and AG 4 HG-I isolates caused the highest severity rating, with the highest DSI for target spot and damping-off, respectively. These results support previous observations (Gurkanli et al 2009;Bacharis et al 2010). R. solani AG 4 HG-I has been reported to be more aggressive than AG 4 HG-II and AG 2-1, causing damping-off symptoms on tobacco seedlings (Gurkanli et al 2009;Bacharis et al 2010).…”

Section: Discussionsupporting

confidence: 96%

“…The predominance of AG 4 HG-I and AG 2-1 isolates was also observed by Gurkanli et al (2009) andBacharis et al (2010) in tobacco seedlings showing symptoms of dampingoff in Turkey and Greece. In this study, isolates obtained from plants with damping-off symptoms were identified as AG 4 HG-III and AG 2-1.…”

Section: Discussionsupporting

confidence: 58%

“…Under wet conditions, especially rainy weather, target spot epidemics may lead to severe losses. However, these conditions are not frequent in Section Editor: Adalberto Café-Filho * Guadalupe E. Mercado Cárdenas mercado.guadalupe@inta.gob.ar 1 tobacco growing areas worldwide (Sherwood 1969;Shew and Melton 1995;Gurkanli et al 2009). Within R. solani, identification is based on the mycelial compatibility/incompatibility between isolates, which enables to assign them to anastomosis groups (AGs) (Sneh et al 1991;Carling 1996).…”

Section: Introductionmentioning

confidence: 98%

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Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Cárdenas

Galván

Barrera³

et al. 2015

Trop. plant pathol.

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In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse.Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.

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Section: Discussionsupporting

confidence: 96%

Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Cárdenas

Galván

Barrera³

et al. 2015

Trop. plant pathol.

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“…In Turkey, however, there are no reports on the presence of BN Rhizoctonia and MN R . solani on perennial plants, but there are many reports on annual plants, including barley and wheat (Demirci, ), corn (Demirci & Kordali, ), johnsongrass (Demirci, Eken, & Zengin, ), legumes (Eken & Demirci, ), potato (Yanar, Yilmaz, Cesmeli, & Coskun, ), onion (Erper et al., ), bean (Erper et al., ), tabocco (Gurkanlı & Ozkoc, ; Gurkanlı, Ozkoc, & Gunduz, ), pepper (Tuncer & Eken, ), winter squash (Erper, Cebi Kilicoglu, Türkkan, & Onder, ). In the present study, we report findings of BN Rhizoctonia AG‐L, AG‐O and AG‐P, to our knowledge, for the first time in Turkey.…”

Section: Discussionmentioning

confidence: 99%

“…Aiello et al (2017) found that most of AG-R, AG-A and AG-G and AG-V isolates as well as all isolates of R. solani AG 4 HG-I were pathogenic to different ornamental nurseries in Italy, but those of BN Rhizoctonia AG-Fb and R. solani AG 2 2-IIIB did not. In Turkey, however, there are no reports on the presence of BN Rhizoctonia and MN R. solani on perennial plants, but there are many reports on annual plants, including barley and wheat (Demirci, 1998), corn (Demirci & Kordali, 1999), johnsongrass (Demirci, Eken, & Zengin, 2001), legumes (Eken & Demirci, 2003), potato (Yanar, Yilmaz, Cesmeli, & Coskun, 2005), onion (Erper et al, 2006), bean (Erper et al, 2011), tabocco Gurkanlı, Ozkoc, & Gunduz, 2011), pepper (Tuncer & Eken, 2013), winter squash (Erper, Cebi Kilicoglu, Türkkan, & Onder, 2016). In the present study, we report findings of BN Rhizoctonia AG-L, AG-O and AG-P, to our knowledge, for the first time in Turkey.…”

Section: Virulence Of Rhizoctonia Isolatesmentioning

confidence: 99%

Characterization and pathogenicity ofRhizoctoniaspp. isolated from kiwifruit in the Middle and Eastern Black Sea region of Turkey

Türkkan

Erper

Kılıçoğlu

et al. 2018

Journal of Phytopathology

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In this study, 58 Rhizoctonia isolates were obtained from plants exhibiting symptoms of root and stem rot in kiwifruit orchards in six different provinces in the Black Sea region of Turkey. The isolates were analysed for their cultural characteristics, anastomosis groups (AGs) and subgroups according to nucleotide sequence variations in the internal transcribed spacer (ITS) regions including the 5.8S gene of nuclear ribosomal DNA (rDNA) and pathogenicity. Based on ITS1‐5.8S‐ITS2 rDNA sequencing, 52 binucleate (BN) Rhizoctonia isolates were identified as belonging to AG‐O (17), AG‐L (1), AG‐G (7), AG‐I (1), AG‐A (9), AG‐Fa (9), AG‐Fb (1), AG‐R (3) and AG‐P (4). Of the remaining 6 isolates, three belonged to multinucleate (MN) R. solani AG 4 HG‐I, one belonged to AG 4 HG‐II, one belonged to AG 5 and one belonged to AG 1‐IB. The sequences of these isolates were aligned and compared with other Rhizoctonia sequences retrieved from GenBank (NCBI), and phylogenetic analysis was used to determine evolutionary relationships among them. All isolates used in the temperature experiments grew between 15–35°C, and their growth was completely inhibited at 5 and 40°C, with a few exceptions. In the pathogenicity tests carried out using 58.6% of the isolates on kiwifruit seedlings, it was determined that there were statistically significant virulence differences among BN and MN Rhizoctonia isolates (p < 0.05). The virulence of BN Rhizoctonia ranged from 0.2 to 4.0, whereas that of MN varied from 2.4 to 4.0. Unlike some isolates of AG‐R, AG 4 HG‐II and AG 1‐IB, which had no significant difference between them in virulence, AG‐P and AG 4 HG‐I isolates significantly reduced plant height, shoot and root dry weights and root length when compared to control plants. To our knowledge, it is the first detailed report of AGs of BN and MN Rhizoctonia, the causal agent of root and stem rot on kiwifruit in Turkey and in the world.

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Characterization of Rhizoctonia spp. Isolates Associated with Damping-Off Disease in Cotton and Tobacco Seedlings in Greece

et al. 2010

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Isolates of Rhizoctonia spp. were obtained during the spring of 2007 from diseased cotton and tobacco seedlings showing damping-off symptoms. The sampled fields were located in the main cotton- and tobacco-cultivating regions of central and northern Greece. Among the 79 isolates obtained from cotton plants, 17 were binucleate and 62 were multinucleate whereas, among the 89 isolates obtained from tobacco plants, 87 were multinucleate and only 2 were binucleate. Characterization of anastomosis groups (AGs) was performed with hyphal anastomosis reactions using tester isolates of known AG groups. Anastomosis reactions in cotton mutlinucleate isolates showed that 54 of them belonged in Rhizoctonia solani AG-4, 6 in AG-7, 1 in AG-2, and 1 in AG-3. In the 87 tobacco multinucleate isolates, anastomosis reactions showed that 70 of them belonged in R. solani AG-2, 16 in AG-4, and 1 in AG-5. In addition, molecular characterization was carried out using the specific ribosomal DNA internal transcribed spacer region, in a randomly selected number of isolates. In cotton, the most prevalent AG was AG-4, with 18 isolates to the subgroup HG-I, 1 isolate to the subgroup HG-II, and 7 isolates to the subgroup HG-III, followed by AG-7 (7 isolates), AG-2-1 (1 isolate), and AG-3 (1 isolate). In tobacco, the most prevalent group was AG-2-1 (70 isolates), followed by AG-4 (6 isolates to the subgroup HG-I and 5 isolates to the subgroup HG-III) and a single isolate belonging to AG-5. Phylogenetic analysis showed that the isolates were distinctly separated based on their AG types. Pathogenicity and aggressiveness of the isolates to several hosts was determined. AG-4 isolates from either cotton or tobacco were the most aggressive on the hosts tested, while AG-2-1 isolates were of moderate aggressiveness and were not pathogenic on barley.

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Molecular and Conventional Identification and Pathogenicity of Rhizoctonia solani Isolates from Tobacco (Nicotiana tabacum L.) in Samsun, Turkey

Cited by 5 publications

References 39 publications

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

Characterization and pathogenicity ofRhizoctoniaspp. isolated from kiwifruit in the Middle and Eastern Black Sea region of Turkey

Characterization of Rhizoctonia spp. Isolates Associated with Damping-Off Disease in Cotton and Tobacco Seedlings in Greece

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