Molecular and cell models of biological effects of heavy ion radiation

Abstract

Many quantitative models have been developed for the biological effectiveness of radiation of different quality. They differ substantially in their assumptions, and a lack of firm knowledge remains as to the detailed nature of the critical early molecular damage. Analyses of microscopic features of the stochastic structures of radiation tracks have led to hypotheses on the importance of clustered damage in DNA and associated molecules. Clustered damage of greater complexity or severity is suggested to be less repairable and therefore to dominate the biological consequences.

Molecular analysis of carbon ion-induced mutations in Arabidopsis thaliana.

“…High LET radiation such as ion particles causes localized, dense ionization within the cell compared to low LET radiation (Kraft et al, 1992). From microdosimetric and radiobiological considerations, it is proposed that high LET radiation would predominantly produce double strand breaks with damaged end groups whose repairability would be low (Hagen, 1994;Goodhead, 1995). Therefore, it seems plausible to expect that high LET radiation is able to generate deletions more frequently than low LET radiation.…”

Gene therapy vectors containing CArG elements from the Egr1 gene are activated by neutron irradiation, cisplatin and doxorubicin

“…However, clustered, less-repairable DSBs, often associated with other types of DNA damage such as base damage and DNA-protein crosslinks, appear to be generated after radiation of high linear energy transfer (LET), such as neutrons. 37,38 Differences in the response to photons and neutrons have been observed in a number of cell types, in particular in the activation of early response genes. 39,40 The c-fos, c-jun and protein kinase C (PKC) genes, for example, were induced by photons, but not by fission neutrons.…”

Analisis keragaman manggis (Garcinia mangostana) diiradiasi dengan sinar gamma berdasarkan penanda ISSR

“…Radiasi dengan LET tinggi dilaporkan lebih menyebabkan penyusunan kembali DNA dengan ukuran delesi/insersi yang lebih kecil dibandingkan radiasi dengan LET rendah. Hal ini terutama disebabkan ketebalan dan lokasi ionisasi yang dihasilkan LET tinggi pada sel yang diradiasi (Goodhead 1995, Shikazono et al 2001.…”