Molecular and Biological Features of Two New Human Squamous and Adenocarcinoma of the Lung Cell Lines

Gasperi‐Campani, Anna; Roncuzzi, Laura; Ricotti, Luca; Lenzi, Laura; Gruppioni, R.; Zini, Nicoletta; Zoli, Wainer; Amadori, Dino

doi:10.1016/s0165-4608(98)00076-4

Cited by 17 publications

(13 citation statements)

References 25 publications

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“…The study was performed in two established NSCLC cell lines (obtained and characterized in our laboratory) representative of different lung cancer histotypes: the CAEP cell line derived from an epidermoid carcinoma and the RAL cell line derived from an adenocarcinoma (Gasperi-Campani et al, 1998). Cells were maintained as a monolayer at 37°C and subcultured weekly.…”

Section: Established Cell Linesmentioning

confidence: 99%

Docetaxel and gemcitabine activity in NSCLC cell lines and in primary cultures from human lung cancer

et al. 1999

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Non-small-cell lung cancer (NSCLC) is considered one of the most chemoresistant tumours, and in a recent overview the pessimism about the absolute survival benefits from chemotherapy was underlined (Non-small Cell Lung Cancer Collaborative Group, 1995). In particular, the meta-analysis revealed a survival benefit of 10% at 1 year in a supportive care setting and an increased median survival of 1.5 months in patients treated with platinum-based chemotherapy regimens, thus emphasizing the need for new, effective drugs and drug combination regimens.Phase I-II clinical studies have shown that new drugs such as gemcitabine and taxanes, used singly

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Section: Established Cell Linesmentioning

confidence: 99%

Docetaxel and gemcitabine activity in NSCLC cell lines and in primary cultures from human lung cancer

et al. 1999

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“…CAEP cells, derived from a squamous carcinoma of the lung, were isolated and established in our laboratory, as previously described [7]. A549, a commercially available cell line derived from primary lung cancer, was obtained from the American Type Culture Collection (ATCC, Rockville, MD).…”

Section: Methodsmentioning

confidence: 99%

In vitro irradiation system for radiobiological experiments

et al. 2013

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BackgroundAlthough two-dimensional (2-D) monolayer cell cultures provide important information on basic tumor biology and radiobiology, they are not representative of the complexity of three-dimensional (3-D) solid tumors. In particular, new models reproducing clinical conditions as closely as possible are needed for radiobiological studies to provide information that can be translated from bench to bedside.MethodsWe developed a novel system for the irradiation, under sterile conditions, of 3-D tumor spheroids, the in vitro model considered as a bridge between the complex architectural organization of in vivo tumors and the very simple one of in vitro monolayer cell cultures. The system exploits the same equipment as that used for patient treatments, without the need for dedicated and highly expensive instruments. To mimic the passage of radiation beams through human tissues before they reach the target tumor mass, 96-multiwell plates containing the multicellular tumor spheroids (MCTS) are inserted into a custom-built phantom made of plexiglass, the material most similar to water, the main component of human tissue.ResultsThe system was used to irradiate CAEP- and A549-derived MCTS, pre-treated or not with 20 μM cisplatin, with a dose of 20 Gy delivered in one session. We also tested the same treatment schemes on monolayer CAEP and A549 cells. Our preliminary results indicated a significant increment in radiotoxicity 20 days after the end of irradiation in the CAEP spheroids pre-treated with cisplatin compared to those treated with cisplatin or irradiation alone. Conversely, the effect of the radio- chemotherapy combination in A549-derived MCTS was similar to that induced by cisplatin or irradiation alone. Finally, the 20 Gy dose did not affect cell survival in monolayer CAEP and A549 cells, whereas cisplatin or cisplatin plus radiation caused 100% cell death, regardless of the type of cell line used.ConclusionsWe set up a system for the irradiation, under sterile conditions, of tumor cells grown in 3-D which allows for the use of the same dose intensities and schedules utilized in clinical practice. This irradiation system, coupled with 3-D cell cultures, has the potential to generate information that could be used to individually tailor radiotherapy.

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“…MCF-7 and LoVo are commercial cell lines obtained from breast and colon carcinomas, respectively, whereas the CAEP cell line was obtained in our laboratory from lung epidermoid carcinoma [13]. Cells were maintained as a monolayer at 37°C in a humidified atmosphere of 5% CO 2 in a DMEM/HAM F12 (1:1) medium supplemented with fetal calf serum (10%), glutamine (2 m M ), nonessential amino acids (1%) and insulin (10 µg/ml).…”

Section: Methodsmentioning

confidence: 99%

Flow-Cytometric Determination of Tumor Cells in Lymph Nodes<footref rid="foot01"><sup>1</sup></footref>

Zoli¹,

Barzanti

Susino

et al. 2002

Oncology

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In solid tumors, metastasis occurs through the dissemination of tumor cells in the bloodstream and the lymphatic system. In particular, lymph node infiltration gives useful prognostic information and represents one of the most important factors for selecting the type of clinical treatment in disease management. Furthermore, the analysis of lymph node infiltration has become important for identifying patients with breast cancer or malignant melanoma who may be candidates for regional lymph node dissection. Tumor cells in lymph nodes are currently identified in tissue sections using morphological and immunohistochemical analyses, but these approaches are time-consuming, and micrometastases may escape detection. The aim of the present study was to define the potential of a flow cytometric (FCM) determination based on cell size and autofluorescence to shorten the time required for lymph node analysis. The sensitivity of the FCM approach, defined on mixtures of tumor cells from established cell lines and peripheral blood lymphocytes (PBL_s) at different concentrations, was 1 tumor cell/1,000 PBL_s. FCM analysis was performed on 89 lymph nodes, 29 from breast, 41 from lung and 19 from colon cancer patients. Agreement between FCM and morphological results, used as gold standard, was observed in 83% of the cases, and there was a 90% sensitivity to the FCM approach for each tumor type. Disagreement was observed for 15 lymph nodes and was due, in the majority of cases (80%), to FCM-positive and morphologically negative results. A large number of patients and a more accurate pathological examination of consecutive histological sections of lymph nodes are needed to further evaluate the validity of the FCM approach.

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Molecular and Biological Features of Two New Human Squamous and Adenocarcinoma of the Lung Cell Lines

Cited by 17 publications

References 25 publications

Docetaxel and gemcitabine activity in NSCLC cell lines and in primary cultures from human lung cancer

Docetaxel and gemcitabine activity in NSCLC cell lines and in primary cultures from human lung cancer

In vitro irradiation system for radiobiological experiments

Flow-Cytometric Determination of Tumor Cells in Lymph Nodes<footref rid="foot01"><sup>1</sup></footref>

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