Molecular and biological characteristics of the infectious bronchitis virus TC07-2/GVI-1 lineage isolated in China

Ren, Mengting; Sheng, Jie; Ma, Tianxin; Xu, Li‐Wen; Han, Zongxi; Li, Huixin; Zhao, Yan; Sun, Jiazhi; Liu, Shengwang

doi:10.1016/j.meegid.2019.103942

Cited by 18 publications

(14 citation statements)

References 48 publications

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“…GI-19 field strains showed different levels of virulence to SPF chickens in experimental infections. Some strains isolated in China were nonpathogenic to SPF chicken (Yan et al, 2017), while others showed low to high pathogenicity to SPF chickens, with morbidity ranging from 40 % to 100 % and mortality ranging between 10 % and 80 % (Table 1) (Liu and Kong, 2004;Zhong et al, 2016;Yan et al, 2017;Xu et al, 2018;Li et al, 2019;Ren et al, 2019aRen et al, ,2019b. The GI-19 virus strain isolated in Malaysia was also highly virulent to SPF chickens with 100 % morbidity and 41.3 % mortality (Khanh et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Tissue samples of tracheas, lungs, bursa of Fabricius, spleen, liver, kidneys, proventriculus, small intestines, large intestines, cecum and cecal tonsils of the dead chickens were fixed in 10 % neutral buffered formalin, embedded in paraffin wax, and sectioned. Immunohistochemistry (IHC) was performed on serial sections prepared from the tissue samples (Ren et al, 2019a(Ren et al, ,2019b. The tissue sections were dewaxed and incubated in citrate buffer (pH 6.0) in a microwave oven at 700-800 W for 10 min and then at 200-300 W for 30 min.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…IBV GI-19 viruses were initially thought to be associated with proventriculitis outbreaks (Wang et al, 1998); however, with progress in time, it was demonstrated that these viruses are responsible for respiratory signs and nephritis (Liu and Kong, 2004;Benyeda et al, 2009;Khanh et al, 2018). Morbidity caused by IBV GI-19 viruses is 100 % and mortality is usually between 5% and 70 % (Liu and Kong et al, 2004;Benyeda et al, 2009;Choi et al, 2009;Zhong et al, 2016;Khanh et al, 2018;Xu et al, 2018;Li et al, 2019;Ren et al, 2019aRen et al, ,2019bYan et al, 2019). The present report describes the isolation and investigation of a highly pathogenic IBV strain (I0305/19) belonging to the GI-19 lineage.…”

Section: Introductionmentioning

confidence: 99%

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A highly pathogenic GI-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism

et al. 2020

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In the present study, an IBV strain I0305/19 was isolated from a diseased commercial broiler flock in 2019 in China with high morbidity and mortality. The isolate I0305/19 was clustered together with viruses in sublineage D of GI-19 lineage on the basis of the complete S1 sequence analysis. Isolate I0305/19 and other GI-19 viruses isolated in China have the amino acid sequence MIA at positions 110-112 in the S protein. Further analysis based on the complete genomic sequence showed that the isolate emerged through at least four recombination events between GI-19 ck/CH/LJS/120848-and GI-13 4/91-like strains, in which the S gene was found to be similar to that of the GI-19 ck/CH/LJS/120848-like strain. Pathological assessment showed the isolate was a nephropathogenic IBV strain that caused high morbidity of 100 % and mortality of 80 % in 1-day-old specificpathogen-free (SPF) chicks. The isolate I0305/19 exhibited broader tropisms in different tissues, including tracheas, lungs, bursa of Fabricius, spleen, liver, kidneys, proventriculus, small intestines, large intestines, cecum, and cecal tonsils. Furthermore, subpopulations of the virus were found in tissues of infected chickens; this finding is important in understanding how the virulent IBV strains can potentially replicate and evolve to cause disease. This information is also valuable for understanding the mechanisms of replication and evolution of other coronaviruses such as the newly emerged SARS-CoV-2.

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Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A highly pathogenic GI-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism

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“…An IBV infection is one of the most important threats to the poultry industry in China despite extensive vaccination programs ( Cook et al, 2012 ). Constant molecular surveillance indicated that at least 16 lineages of IBV strains, as well as a number of recombinants are currently circulating in China ( Ren et al, 2019 ). GI-1/Mass IBV is one of the most important serotypes/lineages, although Mass-type vaccines (e.g., H120, H52, and Ma5) are extensively used to control and prevent IBV outbreaks in China.…”

Section: Discussionmentioning

confidence: 99%

Genetic and antigenic heterogeneity of GI-1/Massachusetts lineage infectious bronchitis virus variants recently isolated in China

et al. 2020

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Four GI-1/Massachusetts-type (GI-1/Mass-type) infectious bronchitis virus ( IBV ) strains were isolated and the complete genomes of these isolates, coupled with the Mass-type live-attenuated vaccine H120 and the Mass-type pathogenic M41 strains, were sequenced in the present study. Our results show that isolates LJL/140820 and I0306/17 may be derived from the Ma5 (another Mass-type live-attenuated vaccine strain) and H120 vaccine strains, respectively. The I1124/16 strain was found to be a M41 variant that likely resulted from accumulated mutations in the genome. Consistently, the results of the virus neutralization (VN) test showed that isolate I1124/16 was antigenically related but slight different from the M41. Our results from the protection experiments pointed out that chickens immunized with H120 failed to eliminate viral shedding after infection with the isolate I1124/16, which was different from that of M41; this result was consistent to the field observation and further implicated that the variant IBV isolate I1124/16 was antigenic different from M41 strain. Furthermore, the I1124/16 was found to have comparable but slightly lower pathogenicity with M41 strain. More studies based on the reverse genetic techniques are needed to elucidate the amino acids in the S1 subunit of spike protein contributing to the altered antigenicity of the isolate I1124/16. In addition, an IBV isolate, LJL/130609, was found to be originated from recombination events between the I1124/16- and Connecticut-like strains. Our results from VN test also showed that isolates LJL/130609 and I1124/16 were antigenic closely related. Hence, there are at least three different genetic evolution patterns for the circulation of the GI-1/Mass-type IBV field strains in China. The differences of vaccines used, the field conditions and genetic pressures between different flocks, likely account for the emergence, evolution patterns and characteristics of the Mass-type IBV strains.

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“…The S1 glycoprotein is associated with virus attachment and is a major target of neutralizing antibodies in chickens [4,11]. The genetic grouping of IBV has been performed based on the nucleotide sequence of the S1 gene [10,13,14,20,21].…”

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Genotyping of infectious bronchitis viruses isolated in Japan during 2008−2019

Mase

Gotou

Inoue

et al. 2021

The Journal of Veterinary Medical Science

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Seventeen isolates of infectious bronchitis virus (IBV) were obtained from various prefectures of Japan during 2008–2019 and genetically analyzed. The IBV isolates were classified into six genetic groups, based on phylogenetic analysis of the S1 gene. The S1 genotypes were distinguishable by a newly developed restriction fragment length polymorphism (RFLP) method using three endonucleases, Hae II, Hpa I, and Fok I. Moreover, the isolates were classified into four genetic groups, based on phylogenetic analysis of the S2 gene. However, novel genetic groups based on a combination of S1 and S2 genotypes, which were undetected previously, were confirmed in this study, indicating that various recombinant IBV strains were prevalent in poultry in Japan.

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Molecular and biological characteristics of the infectious bronchitis virus TC07-2/GVI-1 lineage isolated in China

Cited by 18 publications

References 48 publications

A highly pathogenic GI-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism

A highly pathogenic GI-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism

Genetic and antigenic heterogeneity of GI-1/Massachusetts lineage infectious bronchitis virus variants recently isolated in China

Genotyping of infectious bronchitis viruses isolated in Japan during 2008−2019

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