Molecular and biochemical characterization of a cyanogenic β-glucosidase in the inner bark tissues of rubber tree (Hevea brasiliensis Muell. Arg.)

Weimin, Tian; Zhang, Hua; Yang, Shuguang; Shi, Minjing; Wang, Xuchu; Dai, Longjun; Chen, Yueyi

doi:10.1016/j.jplph.2012.12.019

Cited by 11 publications

(5 citation statements)

References 22 publications

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“…Total RNA was isolated separately from the 14 mixed samples according to the procedure of Tian et al 61. A NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, USA) and Agilent 2100 Bioanalyzer (Agilent, USA) were used to determine the total RNA concentration and quality.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of the Signalling Networks in Coronatine-Induced Secondary Laticifer Differentiation from Vascular Cambia in Rubber Trees

Zhang

Chao

et al. 2016

Sci Rep

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The secondary laticifer in rubber tree (Hevea brasiliensis Muell. Arg.) is a specific tissue within the secondary phloem. This tissue differentiates from the vascular cambia, and its function is natural rubber biosynthesis and storage. Given that jasmonates play a pivotal role in secondary laticifer differentiation, we established an experimental system with jasmonate (JA) mimic coronatine (COR) for studying the secondary laticifer differentiation: in this system, differentiation occurs within five days of the treatment of epicormic shoots with COR. In the present study, the experimental system was used to perform transcriptome sequencing and gene expression analysis. A total of 67,873 unigenes were assembled, and 50,548 unigenes were mapped at least in one public database. Of these being annotated unigenes, 15,780 unigenes were differentially expressed early after COR treatment, and 19,824 unigenes were differentially expressed late after COR treatment. At the early stage, 8,646 unigenes were up-regulated, while 7,134 unigenes were down-regulated. At the late stage, the numbers of up- and down-regulated unigenes were 7,711 and 12,113, respectively. The annotation data and gene expression analysis of the differentially expressed unigenes suggest that JA-mediated signalling, Ca2+ signal transduction and the CLAVATA-MAPK-WOX signalling pathway may be involved in regulating secondary laticifer differentiation in rubber trees.

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Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of the Signalling Networks in Coronatine-Induced Secondary Laticifer Differentiation from Vascular Cambia in Rubber Trees

Zhang

Chao

et al. 2016

Sci Rep

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“…Total RNA was extracted according to the method of Tian et al [ 12 ]. The RNA quality was analyzed by the ultraviolet spectrophotometer, and the quantity was determined by the Thermo Gene Company Limited ND2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA) ( S1 Table ).…”

Section: Methodsmentioning

confidence: 99%

Analysis of Differentially Expressed Genes Associated with Coronatine-Induced Laticifer Differentiation in the Rubber Tree by Subtractive Hybridization Suppression

Zhang¹,

Chen

et al. 2015

PLoS ONE

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The secondary laticifer in the secondary phloem is differentiated from the vascular cambia of the rubber tree (Hevea brasiliensis Muell. Arg.). The number of secondary laticifers is closely related to the rubber yield potential of Hevea. Pharmacological data show that jasmonic acid and its precursor linolenic acid are effective in inducing secondary laticifer differentiation in epicormic shoots of the rubber tree. In the present study, an experimental system of coronatine-induced laticifer differentiation was developed to perform SSH identification of genes with differential expression. A total of 528 positive clones were obtained by blue-white screening, of which 248 clones came from the forward SSH library while 280 clones came from the reverse SSH library. Approximately 215 of the 248 clones and 171 of the 280 clones contained cDNA inserts by colony PCR screening. A total of 286 of the 386 ESTs were detected to be differentially expressed by reverse northern blot and sequenced. Approximately 147 unigenes with an average length of 497 bp from the forward and 109 unigenes with an average length of 514 bp from the reverse SSH libraries were assembled and annotated. The unigenes were associated with the stress/defense response, plant hormone signal transduction and structure development. It is suggested that Ca2+ signal transduction and redox seem to be involved in differentiation, while PGA and EIF are associated with the division of cambium initials for COR-induced secondary laticifer differentiation in the rubber tree.

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“…Some of these proteins have been identified as antioxidative enzymes [17]. Proteins with molecular weights of 13 and 55 kDa were identified in the leaves [61], the latter being especially abundant. Both proteins may be RuBisCo small units [62].…”

Section: Leavesmentioning

confidence: 99%

Valorisation of Proteins from Rubber Tree

WIDYARANI

Coulen

Sanders

et al. 2016

Waste Biomass Valor

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Purpose The objective of this study was to identify the availability, possible applications, and economic potential of proteins that are present in different parts of the rubber tree. Proteins from non-food sources can be used in e.g. animal feed or biochemicals production with no or little competition with food production, rendering them important biobased feedstock. Rubber tree is primarily grown for its latex that is used in rubber production. Indonesia has the largest rubber plantation area that is mostly owned and run by smallholder farmers. Using non-latex fractions from the rubber tree may generate additional income, and increase the economics of rubber plantations in general. Methods Several biomass streams from the rubber tree and subsequent latex processing were considered. Data were compiled from literature, a case study, and interviews with researchers, smallholder farmers, and managers at rubber processing plant and plantation. Results Latex waste streams, seeds, and leaves were considered to have the highest potential based on the amount of available proteins, and processes to isolate proteins from these streams were proposed. Isolation of specific functional properties from natural sources requires complex (and expensive) separation processes and therefore only economically feasible when specific use of the protein(s) for high value applications can be identified. Purification of many interesting proteins from latex fractions has already been described. Processing of seeds and leaves may also yield useful proteins for food, other purposes, and also still unknown high value applications. Conclusions A biorefinery concept can be applied to obtain multiple products from the seeds and leaves, and protein extraction can be performed with available knowledge and technology. Small scale processing can be more beneficial for the farmers, especially if the products are used locally for feed.

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Molecular and biochemical characterization of a cyanogenic β-glucosidase in the inner bark tissues of rubber tree (Hevea brasiliensis Muell. Arg.)

Cited by 11 publications

References 22 publications

Transcriptome Analysis of the Signalling Networks in Coronatine-Induced Secondary Laticifer Differentiation from Vascular Cambia in Rubber Trees

Transcriptome Analysis of the Signalling Networks in Coronatine-Induced Secondary Laticifer Differentiation from Vascular Cambia in Rubber Trees

Analysis of Differentially Expressed Genes Associated with Coronatine-Induced Laticifer Differentiation in the Rubber Tree by Subtractive Hybridization Suppression

Valorisation of Proteins from Rubber Tree

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