Molecular and Bacteriological Study of Enterococcus faecalis Isolated from Different Clinical Sources

Abstract

Enterococci are Gram-positive bacteria that cause serious nosocomial infections, including urinary tract, bloodstream infections and endocarditis. During the period of September 2018 to February 2019, forty four isolates of E. faecalis were isolated from 826 clinical specimens including; 35(5.07%) isolates of E. faecalis from urine, 7(7.60%) isolates from high vaginal swab and 2(8.69%) isolates from blood patients in different hospitals. All isolates that described above were identified depending on cultural criteria, morphological criteria, biochemical tests and further confirmed by Vitek 2 compact systems. The results of eleven antimicrobial against obtained isolates revealed that 100% of E. faecalis were resistant to cefotaxime, vancomycin, amoxicillin and erythromycin. While, it were 100% sensitive to doxycycline, imipenem, and nitrofurantion. Whereas, most isolates were differ in their susceptibility to amikacin, gentamycin, tetracycline and azithromycin. On the other hand, the results of biofilm found that 13.63% of isolates were produce strong biofilm, 54.54% were produce moderate biofilm and 31.81% were produce weak biofilm. The results of molecular analysis by using PCR showed that isolated E. faecalis were carried 97.72%, 90.90%, 63.63% of ebpR, asa1, esp genes respectively.

Vaginal Microbiota Profile in first-trimester miscarriages cases

“…In this study findings are in agreement with references (57,58,59,60) regarding the detection percentages of Enterococcus faecalis, which were reported as 7.6%, 8.14%, 6.2%, and 5.89% respectively. Additionally, our results align with reference (55) in terms of detecting E. coli, Klebsiella spp., and Staphylococcus aureus at percentages of 27.27%, 13.64%, and 22.73% respectively.…”

Multidrug resistance Enterococcus Faecalis isolated from patients with urinary tract infections

“…From this study, we report bla-tem that codes for extended-spectrum β-lactamase as the most prevalent antibiotics resistance genes among our isolates. The result in this study show the presence of bla-TEM gene in high percentage (100%) of E. faecalis isolates, in contrast with other study that conducted by [11] that reported the bla-TEM gene correlated with the origin of E. faecalis, were (53.3%) of the nosocomial infections isolates possessed this gene and (30%) of the endodontic isolates. Other study conducted by [33] reported (70%) of enterococcus have bla-TEM gene.…”

“…Identification of Enterococcus faecalis was performed based on a series of conventional microbiological tests include colonial morphology on blood agar, MacConkey agar, HiCrome™ UTI Agar and other biochemical tests. The 16s rRNA gene was targeted by Polymerase chain reaction (PCR) using specific primers [11] , and VITEK-2 compact system in order to confirm the identity of isolate.…”