Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11

Ramírez, J.R. Rocha; Berberich, Christof; Jaramillo, Andrés; Alonso, Carlos; Vélez, Iván Darío

doi:10.1590/s0074-02761998000200022

Cited by 21 publications

(19 citation statements)

References 30 publications

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“…A second, minor immunoreactive band of 22 kDa, recognised by the anti-KMP-11 antibody in the recombinant KMP-11 preparation (Fig. 4B), is a dimeric form of the protein (Berberich et al 1997, Ramírez et al 1998. Taken together, the results presented in Fig.…”

Section: Kmp-11 Is Released In the Supernatant Of L Amazonensis-infesupporting

confidence: 61%

Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Lacerda

Cysne-Finkelstein

Nunes

et al. 2012

Mem. Inst. Oswaldo Cruz

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In Leishmania amazonensis, kinetoplastid membrane protein-11 (KMP-11) expression increases during metacyclogenesis and is higher in amastigotes than in promastigotes, suggesting a role for this protein in the infection of the mammalian host. We show that the addition of KMP-11 exacerbates L. amazonensis infection in peritoneal macrophages from BALB/c mice by increasing interleukin (IL)-10 secretion and arginase activity while reducing nitric oxide (NO) production. The doses of KMP-11, the IL-10 levels and the intracellular amastigote loads were strongly, positively and significantly correlated. The increase in parasite load induced by KMP-11 was inhibited by anti-KMP-11 or anti-IL-10 neutralising antibodies, but not by isotype controls. The neutralising antibodies, but not the isotype controls, were also able to significantly decrease the parasite load in macrophages cultured without the addition of KMP-11, demonstrating that KMP-11-induced exacerbation of the infection is not dependent on the addition of exogenous KMP-11 and that the protein naturally expressed by the parasite is able to promote it. In this study, the exacerbating effect of KMP-11 on macrophage infection with Leishmania is for the first time demonstrated, implicating it as a virulence factor in L. amazonensis. The stimulation of IL-10 production and arginase activity and the inhibition of NO synthesis are likely involved in this effect

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Section: Kmp-11 Is Released In the Supernatant Of L Amazonensis-infesupporting

confidence: 61%

Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Lacerda

Cysne-Finkelstein

Nunes

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…This statement is not easily reconciled with the immunoprotective capacity of vaccine prototypes based on the KMP-11-coding gene (Ramírez at al. 2001, Basu et al 2005, with the T cell responses to KMP-11 found in leishmaniasis patients (Russo et al 1992) or with the presence of anti-KMP-11 serum antibodies in L. infantum-infected dogs (Berberich et al 1997), as well as in most leishmaniasis patients or asymptomatic Leishmania-infected subjects (Ramírez et al 1998). All of these observations are consistent with the presence of the protein in the amastigote form, the infective stage for the mammalian host.…”

Section: Discussionmentioning

confidence: 97%

Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis

Matos

Faccioli

Cysne-Finkelstein

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…The recombinant kinetoplastid membrane protein-11 (KMP-11) and the recombinant LACK antigen showed enhanced immune modulatory properties both in CL and MCL patients [163]. Moreover, 86 % of asymptomatic but infected individuals in the New World display rKMP-11-specific antibodies in their sera [164], further proof that KMP-11 elicits a humoral response.…”

Section: The A2 Gene Family Is Expressed In Viscerotropicmentioning

confidence: 96%

The genetics of Leishmania virulence

Bifeld

Clos

2015

Med Microbiol Immunol

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The ability of Leishmania parasites to infect and persist in the antigen-presenting cell population of their mammalian hosts is dependent on their ability to gain entry to their host and host cells, to survive the mammalian cell environment, and to suppress or evade the protective immune response mechanisms of their hosts. A multitude of genes and their products have been implicated in each of these virulence-enhancing strategies to date, and we present an overview of the nature and known function of such virulence genes.

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Molecular and Antigenic Characterization of the Leishmania (Viannia) panamensis Kinetoplastid Membrane Protein-11

Abstract: The kinetoplastid membrane protein 11

Cited by 21 publications

References 30 publications

Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis

The genetics of Leishmania virulence

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