Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis

Matos, Denise C. S.; Faccioli, Lanuza A. P.; Cysne-Finkelstein, Léa; Luca, Paula Mello De; Cortê-Real, Suzana; Armôa, Geraldo R. G.; Lemes, Elezer Monte Blanco; Decotè-Ricardo, Débora; Mendonça, Sergio C. F.

doi:10.1590/s0074-02762010000300018

Cited by 23 publications

(20 citation statements)

References 33 publications

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“…Recently, it has been demonstrated that the surface expression of this protein is higher in the amastigotes than in the promastigotes of L. amazonensis. Moreover, in L. amazonensis promastigotes, the surface expression of KMP-11 increases during metacyclogenesis (Matos et al 2010). The increased expression of KMP-11 in metacyclic promastigotes and especially in amastigotes indicates a role for this molecule in the infection of the mammalian host.…”

Section: Discussionmentioning

confidence: 95%

“…Several studies have compared KMP-11 expression between different life cycle stages in some trypanosomatid species (Stebeck et al 1995, Matos et al 2010), but not between species. It would be interesting to know whether KMP-11 plays a similar role in the promotion of infection with other Leishmania species.…”

Section: Discussionmentioning

confidence: 99%

“…Recombinant L. amazonensis KMP-11 -Recombinant L. amazonensis KMP-11 was produced as described by Matos et al (2010). The purified protein was analysed by 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and silver-nitrate staining, which indicated the absence of protein contaminants and the protein was identified with an anti-KMP-11 monoclonal antibody (Cedarlane, Burlington, Canada) with Western blot analysis.…”

Section: Methodsmentioning

confidence: 99%

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Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Lacerda

Cysne-Finkelstein

Nunes

et al. 2012

Mem. Inst. Oswaldo Cruz

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In Leishmania amazonensis, kinetoplastid membrane protein-11 (KMP-11) expression increases during metacyclogenesis and is higher in amastigotes than in promastigotes, suggesting a role for this protein in the infection of the mammalian host. We show that the addition of KMP-11 exacerbates L. amazonensis infection in peritoneal macrophages from BALB/c mice by increasing interleukin (IL)-10 secretion and arginase activity while reducing nitric oxide (NO) production. The doses of KMP-11, the IL-10 levels and the intracellular amastigote loads were strongly, positively and significantly correlated. The increase in parasite load induced by KMP-11 was inhibited by anti-KMP-11 or anti-IL-10 neutralising antibodies, but not by isotype controls. The neutralising antibodies, but not the isotype controls, were also able to significantly decrease the parasite load in macrophages cultured without the addition of KMP-11, demonstrating that KMP-11-induced exacerbation of the infection is not dependent on the addition of exogenous KMP-11 and that the protein naturally expressed by the parasite is able to promote it. In this study, the exacerbating effect of KMP-11 on macrophage infection with Leishmania is for the first time demonstrated, implicating it as a virulence factor in L. amazonensis. The stimulation of IL-10 production and arginase activity and the inhibition of NO synthesis are likely involved in this effect

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Lacerda

Cysne-Finkelstein

Nunes

et al. 2012

Mem. Inst. Oswaldo Cruz

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“…Another Leishmania pathogenicity factor, the kinetoplastid membrane protein-11 (KMP-11), whose expression is increased during metacyclogenesis and in L. amazonensis amastigote stage, was shown to exacerbate BALB/c mice peritoneal macrophages infection by increasing IL-10 secretion [65,66]. KMP-11 was also shown to increase the macrophage arginase activity while reducing the nitric oxide production, thus promoting parasite survival [65].…”

Section: -By the Amastigotementioning

confidence: 99%

Leishmania, the phagosome, and host responses: The journey of a parasite

Séguin¹,

Descoteaux²

2016

Cellular Immunology

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“…This 11-kDa membrane protein was found in different kinetoplastids and therefore named KMP-11 [70]. The protein is expressed during both Leishmania life stages with an increased expression during the metacyclic and amastigote stage [71], suggesting an important role in the mammalian host. This role may be the downregulation of iNOS activity in infected macrophages.…”

Section: Kmp-11mentioning

confidence: 99%

The genetics of Leishmania virulence

Bifeld

Clos

2015

Med Microbiol Immunol

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The ability of Leishmania parasites to infect and persist in the antigen-presenting cell population of their mammalian hosts is dependent on their ability to gain entry to their host and host cells, to survive the mammalian cell environment, and to suppress or evade the protective immune response mechanisms of their hosts. A multitude of genes and their products have been implicated in each of these virulence-enhancing strategies to date, and we present an overview of the nature and known function of such virulence genes.

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Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis

Cited by 23 publications

References 33 publications

Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Kinetoplastid membrane protein-11 exacerbates infection with Leishmania amazonensis in murine macrophages

Leishmania, the phagosome, and host responses: The journey of a parasite

The genetics of Leishmania virulence

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