Molecular analysis of soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination

Schneider, William L.; Damsteegt, V. D.; Stone, Andrew L.; Kuhlmann, Micki M.; Bunyard, Britt A.; Sherman, Diana J.; Graves, Michael V.; Smythers, Gary W.; Smith, O. P.; Hatziloukas, Efstathios

doi:10.1016/j.virol.2011.01.001

Cited by 12 publications

(5 citation statements)

References 30 publications

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“…Isolates of SbDV in the Midwest are D-like and known to infect red clover (11,41), while most isolates found in mid-Atlantic states areY-like or mixed D and Y and found in white clover (7) ( Table I). Isolates obtained from red clover in eastern states (7,28) also were found to be D-like, similar to Midwest isolates. The preference of A. glycines for red clover may be significant in the epidemiology of SbDV since it may serve as an alternative host for SbDV, especially SbDV-D,…”

Section: Discussionmentioning

confidence: 61%

“…Isolates were routinely transferred with A. pisum (pea aphid), Myzus persicae (Sulzer) (green peach aphid), and/or Nearctaphis bakeri (Cowen) (short-beaked clover aphid) (7,11). Some isolates closely align with the dwarfing genotype of SbDV-D, some with the yellowing genotype SbDV-Y, and some were coinfected with both genotypes (28). IVansmission experiments.…”

Section: Iviaterials and Methodsmentioning

confidence: 99%

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Acquisition and Transmissibility of U.S. Soybean dwarf virus Isolates by the Soybean Aphid, Aphis glycines

Damsteegt¹,

Stone²,

Kuhlmann³

et al. 2011

Plant Disease

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Damsteegt, V. D., Stone, A. L., Kublmann, M., Gildow, F E., Domier, L. L., Sherman, D. J., Tian, B., and Schneider, W. L. 2011. Acquisition and transmissibility of U.S. Soybean dwarf virus isolates by tbe soybean aphid. Aphis glycines. Plant Dis. 95:945-950.Soybean dwarf virus (SbDV) exists as several distinct strains based on symptomatology, vector specificity, and host range. Originally characterized Japanese isolates of SbDV were specifically transmitted by Aulacorthum solani. More recently, additional Japanese isolates and endemic U.S. isolates bave been shown to be transmitted by several different apbid species. The soybean aphid. Aphis glycines, tbe only aphid that colonizes soybean, has been shown to be a very inefficient vector of some SbDV isolates from Japan and the United States. Transmission experiments have shown that tbe soybean apbid can transmit certain isolates of SbDV from soybean to soybean and clover species and from clover to clover and soybean witb long acquisition and inoculation access periods. Although transmission of SbDV by tbe soybean apbid is very inefficient, tbe large soybean apbid populations that develop on soybean may have epidemiological potential to produce serious SbDV-induced yield losses.Soybean dwarf virus (SbDV) is the causal agent of an economically important disease of soybean (Glycine max (L.) Merr.) in Japan (34-37) and a minor disease of other crops elsewhere (21). The causal virus has been transmitted to more than 50 species of forage legumes, pulse crops, and other broadleaf plants representing three families (1,5,6,18,21).As a member of the Luteoviridae, SbDV is transmitted only by apbids in a circulative, persistent manner. It is pbloem-limited and occurs in low concentration in plants (32). Tbe virus consists of several distinct strains based on symptomatology in soybeans, specificity of transmission by apbid species, physiochemical properties, and nucleotide sequence (5,27-30,37). The originally described Japanese soybean strains (SbDV-DS and SbDV-YS), hereinafter called SbDV-D and SbDV-Y, and a subterranean clover red-leaf virus (SCRLV, now synonymous with SbDV) described in Australia (20) were transmitted almost exclusively by Aulacorthum solani Kaltenbacb. An isolate serologically related to SCRLV found in California (19), and other related isolates found worldwide, were transmitted by Acyrthosiphon pisum (Harris) and other aphid species (7,11,16,22,23), and not by A. solani. More recently, dwarfing and yellowing strains of SbDV (SbDV-DP and SbDV-YP) have been described from Japan tbat were transmitted by Acyrthosiphon pisum (37). Except for one report (15), all earlier attempts to transmit strains of SbDV by tbe soybean aphid Aphis glycines Matsumora have been unsuccessful (11,32,41). The soybean apbid has been reported to transmit several plant vinises (4,11) including tbe Indonesian Soybean dwarf virus (17). Tbe Indonesian SbDV was shown to be serologically distinct from Japanese SbDV isolates, and no further reports about this virus could be found.Corresp...

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Section: Discussionmentioning

confidence: 61%

Section: Iviaterials and Methodsmentioning

confidence: 99%

Acquisition and Transmissibility of U.S. Soybean dwarf virus Isolates by the Soybean Aphid, Aphis glycines

Damsteegt¹,

Stone²,

Kuhlmann³

et al. 2011

Plant Disease

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“…In theory, each clone represented a unique viral RNA, and comparing the sequences of the viral clones to the consensus sequence provided a snapshot of the genetic diversity generated within a given viral population. Using these clones as a representative sample of viral populations, mutation frequency and the percentage of mutated viral clones were used as indicators of population diversity (Schneider et al 2011). Control reactions were done using in vitro transcripts as the template RNA to estimate the level of variability introduced by transcription, RT, and thermal cycling.…”

Section: Sbdv Genetic Diversity and Mutation Frequency In Plant Hostsmentioning

confidence: 99%

“…. Soybean aphids and SbDV isolates transmitted by soybean aphids were also discovered in the eastern US (Schneider et al 2011). However, SbDV infections in fields were limited, and only occurred on the very edges of the fields.…”

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confidence: 99%

Aphid vectors impose a major bottleneck on Soybean dwarf virus populations for horizontal transmission in soybean

et al. 2019

Self Cite

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Many RNA viruses have genetically diverse populations in a single host. Important biological characteristics may be related to the levels of diversity, including adaptability, host specificity, and host range. Shifting the virus between hosts might result in a change in the levels of diversity associated with the new host. The level of genetic diversity for these viruses is related to host, vector and virus interactions, and understanding these interactions may facilitate the prediction and prevention of emerging viral diseases. It is known that luteoviruses have a very specific interaction with aphid vectors. Previous studies suggested that there may be a tradeoff effect between the viral adaptation and aphid transmission when Soybean dwarf virus (SbDV) was transmitted into new plant hosts by aphid vectors. In this study, virus titers in different aphid vectors and the levels of population diversity of SbDV in different plant hosts were examined during multiple sequential aphid transmission assays. The diversity of SbDV populations revealed biases for particular types of substitutions and for regions of the genome that may incur mutations among different hosts. Our results suggest that the selection on SbDV in soybean was probably leading to reduced efficiency of virus recognition in the aphid which would inhibit movement of SbDV through vector tissues known to regulate the specificity relationship between aphid and virus in many systems.

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“…A survey of soybean diseases in northern Illinois also identified two SbDV-D strains [7]. Recently, the presence of mixed infections of both D and Y strains were confirmed in the eastern United States [8], and several U.S. isolates were found to be transmitted by A. glycines [9] Currently, there are no SbDV resistant commercial cultivars available. More recently, it has been found that the Rsdv1 quantitative trait loci gene was responsible for resistance of an Indonesian cultivar ‘Wilis’ [10], but the mechanism of such gene is still unclear [11].…”

Section: Introductionmentioning

confidence: 99%

Host Adaptation of Soybean Dwarf Virus Following Serial Passages on Pea (Pisum sativum) and Soybean (Glycine max)

Tian

Gildow

Stone

et al. 2017

Viruses

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Soybean Dwarf Virus (SbDV) is an important plant pathogen, causing economic losses in soybean. In North America, indigenous strains of SbDV mainly infect clover, with occasional outbreaks in soybean. To evaluate the risk of a US clover strain of SbDV adapting to other plant hosts, the clover isolate SbDV-MD6 was serially transmitted to pea and soybean by aphid vectors. Sequence analysis of SbDV-MD6 from pea and soybean passages identified 11 non-synonymous mutations in soybean, and six mutations in pea. Increasing virus titers with each sequential transmission indicated that SbDV-MD6 was able to adapt to the plant host. However, aphid transmission efficiency on soybean decreased until the virus was no longer transmissible. Our results clearly demonstrated that the clover strain of SbDV-MD6 is able to adapt to soybean crops. However, mutations that improve replication and/or movement may have trade-off effects resulting in decreased vector transmission.

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Molecular analysis of soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination

Cited by 12 publications

References 30 publications

Acquisition and Transmissibility of U.S. Soybean dwarf virus Isolates by the Soybean Aphid, Aphis glycines

Acquisition and Transmissibility of U.S. Soybean dwarf virus Isolates by the Soybean Aphid, Aphis glycines

Aphid vectors impose a major bottleneck on Soybean dwarf virus populations for horizontal transmission in soybean

Host Adaptation of Soybean Dwarf Virus Following Serial Passages on Pea (Pisum sativum) and Soybean (Glycine max)

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