Molecular analysis of secretor type α(1,2)‐fucosyltransferase gene mutations in the Chinese and Thai populations

Abstract

The FUT2 genes encoding for the phenotypes Le(a+b+) and Le(a+b-) are the same. The function and character of the mutant enzyme may play an important role in the phenotype. The methods used in this study are clinically applicable in population studies of the FUT2 gene polymorphism to explore relationships among different ethnic groups and correlations between phenotype and genotype.

Polymorphism of the human ABO‐Secretor locus (FUT2) in four populations in Asia: indication of distinct Asian subpopulations

“…We found 16 single nucleotide polymorphisms (SNPs) (Fig. 1), of which 14 sites have been previously reported in different populations Yu et al 1995 ;Henry et al 1996 b, c ;Koda et al 1996 ;Liu et al 1998 ;Chang et al 1999 ;Pang et al 2000). The additional two sites with a base substitution, G539A (Arg180Gln) and synonymous C759A (Gly253Gly), were first identified in the present study.…”

“…Therefore, the se$!# seemed to originate from a certain South Asian population. The C571T substitution of se$&( ,&(" allele was first found in one Japanese subject, and Polynesians (Koda et al 1996 ;Henry et al 1996 c), and recently in Philippine-Taiwanese and Samoans with a high frequency (Peng et al 1999 ;Pang et al 2000). It appears, however, not to be so common in other Asian populations.…”

“…The se%#) allele, which contains a nonsense mutation G428A and a further six base substitutions compared to the reference allele reported by Kelly et al (1995) (see Fig. 1), is predominant in Africans and Europeans Liu et al 1998) (Koda et al 1996) and some, but not all, individuals homozygous for the se$&( ,$)& showed the Le(ajbj) phenotype on red cells, Yu et al (1995) and (Henry et al 1996 b, c ;Chang et al 1999 ;Peng et al 1999 ;Pang et al 2000). The Se%!…”

“…In the context of the FUT2 locus of Asians, several nonfunctional alleles and their frequencies provide important information about the genetic history and subpopulation constitution in the Asian continent. The A385T substitution in the se$&( ,$)& allele was thought to have occurred after the divergence of the Mongoloid from the Caucasoid, because to date the se$&( ,$)& has been found only within Asia and the Pacific islands Koda et al 1996 ;Yu et al 1996Yu et al , 1999Liu et al 1998Liu et al , 1999Chang et al 1999 ;Peng et al 1999 ;Pang et al 2000). (Koda et al 2001).…”

Molecular analysis of mutations and polymorphisms of the Lewis secretor type α(1,2)-fucosyltransferase gene reveals that Taiwan aborigines are of Austronesian derivation

“…Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the FUT2 mutations and polymorphisms was carried out as described previously Chang et al 1999). For those subjects without any of the mutations mentioned above or in uncertain cases, a pair of primers specific for the Sec 2 DNA segment encoding the secretor α(1,2)-fucosyltransferase gene was used to amplify the coding region of the FUT2 gene.…”