Molecular Analysis of Persistent Periradicular Lesions and Root Ends Reveals a Diverse Microbial Profile

Prevalence of Treponemaspp. in endodontic retreatment-resistant periapical lesions

“…7,8,9,16 The majority of the retreatment-resistant apical lesions enclosed bacterial DNA, with the exception of two samples. Similar findings were reported by Subramanian and Mickel, 17 who found that 18.2% of the excised lesion did not present bacterial DNA. Either these samples were bacteria free, or bacterial colonies were located in the specific part of the samples that had been sent for histopathological examination.…”

Exploring Bacterial Diversity of Endodontic Microbiota by Cloning and Sequencing 16S rRNA

“…The assessment of the human microbioma by metagenomic approaches revealed that these techniques are sensitive and specific (6,11) to evaluate the bacterial diversity of root canal infections (10,(12)(13)(14)(15). These studies have shown that the prevalence of yet non-cultivable bacteria remarks over 50% of the microbiota in primary endodontic infections (10,14), in persistent periradicular lesions (15), or in mixed endodontic infections (16).…”

Effectiveness of high-power diode laser associated to chemical auxiliary substance on decontamination of root canal system infected with Enterococcus faecalis – an in vitro study

“…Enterococcus faecalis is usually found in persistent lesions even after root canal treatment [14][15][16] . Furthermore, this microorganism shows the ability to successfully colonize the root canal system in the biofilm form 3,17 , which justifies its selection for the tested specie of the present study.…”