Exploring Bacterial Diversity of Endodontic Microbiota by Cloning and Sequencing 16S rRNA

Ribeiro, A. S.; Matarazzo, Flávia; Faveri, Marcelo; Zezéll, Denise Maria; Mayer, Márcia Pinto Alves

doi:10.1016/j.joen.2011.04.007

Cited by 50 publications

(42 citation statements)

References 46 publications

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“…Fusobacteria was the third most abundant phylum, and Bacteroidetes was the third richest phylum. Previous studies with culture methods or cloning and Sanger sequencing demonstrated that Firmicutes and Bacteroidetes are the most dominant and/or represented phyla in endodontic infections (26)(27)(28)(29). However, the greatest advantage of the pyrosequencing approach used in this study over the previous methods is the depth of coverage, which might have contributed to differences.…”

Section: Discussionmentioning

confidence: 85%

Pyrosequencing Analysis of the Apical Root Canal Microbiota

Siqueira

Alves

Rôças

2011

Journal of Endodontics

115

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Section: Discussionmentioning

confidence: 85%

Pyrosequencing Analysis of the Apical Root Canal Microbiota

Siqueira

Alves

Rôças

2011

Journal of Endodontics

115

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“…nlmnih.gov) through the basic local alignment search tool (BLAST). Sequences with more than 4 ambiguous characters or shorter than 500 bases were discarded, and it was considered at least 98% similarity level (15).…”

Section: S Rrna Sequencingmentioning

confidence: 99%

Molecular Identification of Cultivable Bacteria From Infected Root Canals Associated With Acute Apical Abscess

et al. 2016

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The objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST. Strains not identified by sequencing were submitted to clonal analysis. The association of microbiological findings with clinical features and the association between microbial species were also investigated. Fifty-nine different cultivable bacteria were identified by 16S rRNA gene sequencing, belonging to 6 phyla, with an average number of 6 species per root canal. Molecular approaches allowed identification of 99% of isolates. The most frequently identified bacteria were Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, and Peptostreptococcus stomatis. Positive association was found between Prevotella buccae and Pseudoramibacter alactolyticus and between Parvimonas micra and Prevotella nigrescens (both p<0.05). It was concluded that the microbiota of infected root canals associated with acute apical abscess is diverse and heterogeneous, composed mainly of anaerobic Gram-negative bacteria, with the great majority belonging to the phyla Firmicutes and Bacteroidetes.

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“…The difficulties in cultivating some of the putative endodontic pathogens have delayed the better comprehension of the endodontic microbiota composition as well as possible associations between specific species and clinical situations. With the recent advances in microbial identification methods including more accurate and sensitivity molecular techniques, the multispecies nature of endodontic bacterial communities has been disclosed (26)(27)(28). It has been suggested that endodontic microbiota can differ according to environmental pressures (20,21).…”

Section: Discussionmentioning

confidence: 99%