Molecular Analysis of Parapoxvirus Detected in Eight Calves in Japan

Yaegashi, Gakuji; Sasaki, Ieharu; Chiba, S; Murakami, Kenji

doi:10.1292/jvms.12-0456

Cited by 16 publications

(11 citation statements)

References 12 publications

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“…Previously, there has only been one report describing the PCR detection of PCPV DNA in Japan, in which PCPV DNA was detected from oral lesions in a calf in Iwate Prefecture, in the northern part of Japan, but virus isolation was unsuccessful [ 15 ]. Notably, the partial-length sequence of the B2L gene determined from the PCR product (accession no.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, since parapoxviruses cross-react antigenically and two similar strains infected cattle in different locations in separate years, YG2828-like PCPV might be spreading among the cattle population in Japan. It is known that cattle are frequently infected with parapoxvirus subclinically [ 15 ] and PCPV has zoonotic potential [ 2 ]. Therefore, we recommend to wear gloves for people with regular exposure to cattle mucosa.…”

Section: Discussionmentioning

confidence: 99%

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First isolation and genetic characterization of pseudocowpox virus from cattle in Japan

Ohtani

Yokoyama

Narushige

et al. 2017

Virol J

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BackgroundPseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined.ResultsIn May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxvirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated virus was classified into PCPV.ConclusionThis case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan.Electronic supplementary materialThe online version of this article (10.1186/s12985-017-0840-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

First isolation and genetic characterization of pseudocowpox virus from cattle in Japan

Ohtani

Yokoyama

Narushige

et al. 2017

Virol J

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“…Parapoxviruses cause papules and erosions on the muzzle, oral mucosa, and udder [6] and may cause high morbidity and loss of productivity [7]. Parapoxvirus infections may also be asymptomatic [8], and can infect humans working in close contact with infected animals [7,9].…”

Section: Introductionmentioning

confidence: 99%

First detection and molecular characterisation of pseudocowpox virus in a cattle herd in Zambia

Ziba

Chitala

Settypalli

et al. 2020

Virol J

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Background Pseudocowpox virus (PCPV) of the genus Parapoxvirus in the family Poxviridae causes pseudocowpox in cattle worldwide and presents a zoonotic concern. Most poxviruses produce diseases of similar clinical signs in affected animals, which are impossible to differentiate clinically or by serology. It is, therefore, vital to use molecular assays to rapidly identify the causative agents of poxvirus infections. This study aimed to detect, diagnose, and characterize the causative agent of pox-like skin lesions in a cattle herd in Zambia, initially suspected to be infected with Lumpy Skin Disease virus. Methods We used a High-Resolution Melting (HRM) analysis assay to detect the PCPV genome and sequenced the major envelope protein (B2L gene) for comparative sequence and phylogenetic analysis. Results Our field investigations showed cattle presenting atypical skin lesions and high morbidity within the herd. The laboratory diagnosis, based on the HRM assay revealed PCPV DNA in the samples. Phylogenetic and comparative sequence analyses confirmed PCPV in the samples and revealed genomic differences between samples collected in 2017 and 2018 from the same farm. Conclusion Our work is the first documented report of PCPV in Zambia. It shows the strength of molecular methods to diagnose pox-like infections in cattle and discriminate between diseases causing similar clinical signs. This rapid and accurate diagnosis improves the response time for more accurate veterinary interventions.

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“…Parapoxviruses cause papules and erosions on the muzzle, oral mucosa, and udder [6] and may cause high morbidity and loss of productivity [7]. Parapoxvirus infections may, however, also be asymptomatic [8].…”

Section: Introductionmentioning

confidence: 99%

First Detection and Molecular Characterisation of Pseudocowpox Virus in a Cattle Herd in Zambia

Ziba

Chitala

Settypalli³

et al. 2020

Preprint

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Background: Pseudocowpox virus (PCPV) of the genus Parapoxvirus in the family Poxviridae causes pseudocowpox in cattle worldwide and presents a zoonotic concern. Most poxviruses produce diseases of similar clinical signs in affected animals, which are impossible to differentiate clinically or by serology. It is, therefore, vital to use molecular assays to identify the causative agents of poxvirus infections rapidly. This study aimed to detect, diagnose, and characterize the causative agent of pox-like skin lesions in a cattle herd in Zambia, initially suspected to be infected with the Lumpy Skin Disease virus.Methods: We used a high resolution melting (HRM) analysis assay to detect the PCPV genome and sequenced the major envelope protein (B2L) gene for comparative sequence and phylogenetic analysis. Results: Our field investigations showed cattle presenting atypical skin lesions and high morbidity within the herd. The laboratory diagnosis based on an HRM assay revealed the PCPV genome in the samples. Phylogenetic and comparative sequence analyses confirmed PCPV in the samples. They revealed genomic differences between samples collected in 2017 and 2018 from the same farm.Conclusion: Our work is the first documented report of PCPV in Zambia. It shows the strength of molecular methods to diagnose pox-like infections in cattle and discriminate between diseases causing similar clinical signs for better veterinary interventions.

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Molecular Analysis of Parapoxvirus Detected in Eight Calves in Japan

Cited by 16 publications

References 12 publications

First isolation and genetic characterization of pseudocowpox virus from cattle in Japan

First isolation and genetic characterization of pseudocowpox virus from cattle in Japan

First detection and molecular characterisation of pseudocowpox virus in a cattle herd in Zambia

First Detection and Molecular Characterisation of Pseudocowpox Virus in a Cattle Herd in Zambia

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