Molecular analysis of microbial diversity in the Zavarzin Spring, Uzon Caldera, Kamchatka

Gumerov, Vadim M.; Mardanov, Andrey V.; Beletsky, Alexey V.; Bonch-Osmolovskaya, E. A.; Ravin, Nikolai V.

doi:10.1134/s002626171102007x

Cited by 41 publications

(29 citation statements)

References 23 publications

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“…Most of these phylogenetic studies have used 16S rRNA clone libraries in combination with cultivation methods. The results of these studies [2], [7], [11], [14], [16], [19], [22], [24]–[26] have expanded our knowledge of thermophilic microorganisms that commonly inhabit thermal springs. Thermophilic or hyperthermophilic Bacteria are commonly present in high-temperature hot springs (>75°C) [19], [27], [28] such as the phyla Aquificae, Deinococcus-Thermus, Thermodesulfobacteria, Thermotogae, and some thermophilic members within Proteobacteria and Firmicutes.…”

Section: Introductionmentioning

confidence: 99%

Control of Temperature on Microbial Community Structure in Hot Springs of the Tibetan Plateau

et al. 2013

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The Tibetan Plateau in Northwest China hosts a number of hot springs that represent a biodiversity hotspot for thermophiles, yet their diversity and relationship to environmental conditions are poorly explored in these habitats. In this study we investigated microbial diversity and community composition in 13 Tibetan hot springs with a wide range of temperatures (22.1–75°C) and other geochemical conditions by using the 16S rRNA gene pyrosequencing approach. Bacteria (108–1011 copy/g; 42 bacterial phyla) in Tibetan hot springs were more abundant and far more diverse than Archaea (107–1010 copy/g; 5 archaeal phyla). The dominant bacterial phyla systematically varied with temperature. Moderate temperatures (75–66°C) favored Aquificae, GAL35, and novel Bacteria, whereas low temperatures (60–22.1°C) selected for Deinococcus-Thermus, Cyanobacteria, and Chloroflexi. The relative abundance of Aquificae was correlated positively with temperature, but the abundances of Deinococcus-Thermus, Cyanobacteria, and Chloroflexi were negatively correlated with temperature. Cyanobacteria and Chloroflexi were abundant in Tibetan hot springs and their abundances were positively correlated at low temperatures (55–43°C) but negatively correlated at moderate temperatures (75–55°C). These correlation patterns suggest a complex physiological relationship between these two phyla. Most archaeal sequences were related to Crenarchaeota with only a few related to Euryarchaeota and Thaumarchaeota. Despite the fact that microbial composition in Tibetan hot springs was strongly shaped by temperature, microbial diversity (richness, evenness and Shannon diversity) was not significantly correlated with temperature change. The results of this study expand our current understanding of microbial ecology in Tibetan hot springs and provide a basis for a global comparison.

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Section: Introductionmentioning

confidence: 99%

Control of Temperature on Microbial Community Structure in Hot Springs of the Tibetan Plateau

et al. 2013

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“…Limited to the case of thermophiles, cyanobacteria are found in many high temperature springs, where filamentous genera produce multi-layered sheets and streamer morphologies. Organics produced by cyanobacterial mats can also allow the existence of thermophilic organotrophs, such as the genera Calditerrivibrio, Desulfurella, and Thermus, such as in Kamchatka [56] and other hot spring regions.…”

Section: Hydrothermalism: Early Earth and Present-daymentioning

confidence: 99%

How Do Modern Extreme Hydrothermal Environments Inform the Identification of Martian Habitability? The Case of the El Tatio Geyser Field

Barbieri

Cavalazzi

2014

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Despite the success in knowledge gained by the Mars missions in the last two decades, the search for traces of life on Mars is still in progress. The reconstruction of (paleo-) environments on Mars have seen a dramatic increase, in particular with regard to the potentially habitable conditions, and it is now possible to recognize a significant role to subaerial hydrothermal processes. For this reason, and because the conditions of the primordial Earth-when these extreme environments had to be common-probably resembled Mars during its most suitable time to host life, research on terrestrial extreme hydrothermal habitats may assist in understanding how to recognize life on Mars. A number of geological and environmental reasons, and logistics opportunities, make the geothermal field of El Tatio, in the Chilean Andes an ideal location to study.

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“…Metagenomic analysis [39] has been used to evaluate the diversity of microorganisms in marine ecosystems [40]–[44], thermal springs [45], [46], and fresh water bodies [47], including sites with gas hydrate-bearing sediments in Lake Baikal [48].…”

Section: Introductionmentioning

confidence: 99%

The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal

et al. 2013

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Insight into the role of bacteria in degradation of diatoms is important for understanding the factors and components of silica turnover in aquatic ecosystems. Using microscopic methods, it has been shown that the degree of diatom preservation and the numbers of diatom-associated bacteria in the surface layer of bottom sediments decrease with depth; in the near-bottom water layer, the majority of bacteria are associated with diatom cells, being located either on the cell surface or within the cell. The structure of microbial community in the near-bottom water layer has been characterized by pyrosequencing of the 16S rRNA gene, which has revealed 149 208 unique sequences. According to the results of metagenomic analysis, the community is dominated by representatives of Proteobacteria (41.9%), Actinobacteria (16%); then follow Acidobacteria (6.9%), Cyanobacteria (5%), Bacteroidetes (4.7%), Firmicutes (2.8%), Nitrospira (1.6%), and Verrucomicrobia (1%); other phylotypes account for less than 1% each. For 18.7% of the sequences, taxonomic identification has been possible only to the Bacteria domain level. Many bacteria identified to the genus level have close relatives occurring in other aquatic ecosystems and soils. The metagenome of the bacterial community from the near-bottom water layer also contains 16S rRNA gene sequences found in previously isolated bacterial strains possessing hydrolytic enzyme activity. These data show that potential degraders of diatoms occur among the vast variety of microorganisms in the near-bottom water of Lake Baikal.

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Molecular analysis of microbial diversity in the Zavarzin Spring, Uzon Caldera, Kamchatka

Cited by 41 publications

References 23 publications

Control of Temperature on Microbial Community Structure in Hot Springs of the Tibetan Plateau

Control of Temperature on Microbial Community Structure in Hot Springs of the Tibetan Plateau

How Do Modern Extreme Hydrothermal Environments Inform the Identification of Martian Habitability? The Case of the El Tatio Geyser Field

The Structure of Microbial Community and Degradation of Diatoms in the Deep Near-Bottom Layer of Lake Baikal

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