Molecular Analysis of
            <i>Ds</i>
            Controlling Element Mutations at the
            <i>Adh1</i>
            Locus of Maize

Sutton, W.D.; Gerlach, W. L.; Peacock, W. James; Schwartz, Drew

doi:10.1126/science.223.4642.1265

Cited by 204 publications

(111 citation statements)

References 21 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…Transactivation of inactive, Ds-like, elements has helped in elucidating transposition factors and could be useful for controlled transposon tagging [32]. The deleted forms of the transposable elements Tam3 and Ac might be used to set up artificial two-element systems.…”

Section: Discussionmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

View full text Add to dashboard Cite

Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Ac excises from the HPTII gene with comparable frequencies (30~o) in both plant species. When the /~-glucuronidase (GUS) gene was used to detect transposition of Tam3, a significantly lower excision frequency (13~o) was found in both plant species. It could be shown that deletion of parts of the transposable elements Tam3 and Ac, removing either one of the terminal inverted repeats (TIR) or part of the presumptive transposase coding region, abolished the excision from the marker genes. This demonstrates that excision of the transposable element Tam3 in heterologous plant species, as documented for the autonomous element Ac, also depends on both properties. Southern blot hybridization shows the expected excision pattern and the reintegration of Tam3 and Ac elements into the genome of tobacco plants.

show abstract

Section: Discussionmentioning

confidence: 99%

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

et al. 1989

View full text Add to dashboard Cite

show abstract

“…(ii) In the case of a cauliflower mosaic virus strain which contains a mutation resulting in a partial processing of the cauliflower mosaic virus 35S RNA (in the wild-type strain the 35S RNA does not appear to be spliced), it is one of the most A+U-rich RNA regions that is spliced out (18). (iii) Transposable element sequences located in the coding regions of different maize genes are frequently spliced out, partially restoring a wild-type phenotype (21,44,47,51). In most instances, the A+U content of these sequences is high.…”

mentioning

confidence: 99%

Nuclear pre-mRNA processing in plants: distinct modes of 3'-splice-site selection in plants and animals.

Wiebauer¹,

Herrero²,

Filipowicz³

1988

Mol. Cell. Biol.

View full text Add to dashboard Cite

, 1986) has suggested that differences in mRNA splicing processes exist between plants and animals. To gain more information about the specificity of plant pre-mRNA processing, we have compared the splicing of the soybean leghemoglobin pre-mRNA with that of the human I-globin pre-mRNA in transfected plant (Orychophragmus violaceus and Nicotiana tabacum) protoplasts and mammalian (HeLa) cells. Of the three introns of leghemoglobin pre-mRNA, only intron 2 was correctly and efficiently processed in HeLa cells. The 5' splice sites of the remaining two introns were faithfully recognized, but correct processing of the 3' sites took place only rarely (intron 1) or not at all (intron 3); cryptic 3' splice sites were used instead. While the first intron in human ,-globin pre-mRNA was not spliced in transfected plant protoplasts, intron 2 processing occurred at a low level, indicating that some mammalian introns can be recognized by the plant intron-splicing machinery. However, excision of intron 2 proved to be incorrect, involving the authentic 5' splice site and a cryptic 3' splice site. Our results indicate that the mechanism of 3'-splice-site selection during intron excision differs between plants and animals. This conclusion is supported by analysis of the 3'-splice-site consensus sequences in animal and plant introns which revealed that polypyrimidine tracts, characteristic of animal introns, are not present in plant pre-mRNAs. It is proposed that an elevated AU content of plant introns is important for their processing.The splicing of nuclear pre-mRNAs in eucaryotes is a complex multistep process (for reviews, see references 16 and 33). The pre-mRNA is first assembled into a large ribonucleoprotein complex called the spliceosome. In mammalian systems its assembly requires, in addition to the appropriate signals in the RNA substrate, the heterogeneous nuclear ribonucleoprotein polypeptides, small nuclear ribonucleoprotein particles Ul, U2, U4 to U6, and US, and several other less-defined soluble proteins. Excision of an intron (IVS) then occurs in two stages. First, the pre-mRNA is cleaved at the intron 5' border to release exon 1 (Exl). An intron-Ex2 branched intermediate (a lariat) is formed simultaneously. In the second step, cleavage at the 3' splice site and ligation of the two exons occur, accompanied by the release of the lariat intron.Although much information is now available about spliceosome assembly and the importance of the splice junction and branch point consensus sequences in splicing (for reviews, see references 16 and 33), little is known about the structural features which distinguish introns from exons and which establish introns as the sequences to be removed from pre-mRNA. It is evident that the consensus nucleotides are in most instances not sufficient for this purpose; similar sequences are frequently present elsewhere in RNA but are not recognized. It has been proposed that the spatial structure of pre-mRNA may be an important factor in determining the choice of splice sites for processing (...

show abstract

“…The integration of excised Ds::HPT elements into the rice genome was demonstrated by analyzing Ds::HPT sequences in the hygromycin-resistant caUi which were obtained by culturing transfected protoplasts. In one-third of the resistant calli, the integration of excised Ds::HPTwas found and 8 bp direct repeats, which are produced as a consequence of duplication of a target site upon integration of Ac/Ds (D6ring et aL, 1984;Pohlman et aL, 1984;Sutton et aL, 1984), flanked the integrated Ds::HPT. The analysis of the empty target site sequence clearly showed that 8 bp direct repeats were produced upon integration of Ds::HPT.…”

Section: Discussionmentioning

confidence: 99%

“…Eight base pair direct repeats flank the Ds in five out of six calli. Because Ac/Ds elements induce duplication of a target site of 8 bp upon integration (D6ring et al, 1984;Pohlman et al, 1984;Sutton et aL, 1984), the 8 bp direct repeats observed here were likely to have been generated upon integration of Ds::HPT. This was verified by analyzing the integration site in untransformed rice DNA ( Figure 5).…”

Section: Transformation Of Rice Protoplasts By Ds::h Ptmentioning

confidence: 95%

Transposition of the maize Ds element from a viral vector to the rice genome

Sugimoto

Otsuki²,

Saji³

et al. 1994

The Plant Journal

View full text Add to dashboard Cite

SummaryThe geminivirus miecanthus streak virus (MiSV) was used as a gene vector to study the transposition of the maize D$ element in rice protoplasts. Efficient excision of the Ds from the MiSV vector was observed only when the MiSV vector was allowed to replicate and the plasmid expressing the transposese gene encoded by Ac was co-transfected. Under the same condition, the Ds carrying a hygromycin phosphotransfersse gene (Ds::HPT) was also efficiently excised. Hygromycin-rasistant calli were obtained by culturing these transfected protoplasts in order to examine the transpmdtion of the excimKI Ds::HPTinto the rice genome. In five out of 16 calli examined, the Ds::HPT, but not the vector sequence, was integrated into the rice genome and 8 bp target site duplication typical of AciDs transposition was generated. These results show that the D$::HPT inserted in the MiSV vector transposed directly into the rice genome. This demonstrates the direct transposition of s cloned plant transposable element into the plant genome. Implications of these finding are discussed.

show abstract

Molecular Analysis of Ds Controlling Element Mutations at the Adh1 Locus of Maize

Cited by 204 publications

References 21 publications

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants

Nuclear pre-mRNA processing in plants: distinct modes of 3'-splice-site selection in plants and animals.

Transposition of the maize Ds element from a viral vector to the rice genome

Contact Info

Product

Resources

About