Molecular Analysis of Desiccation Tolerance in the Resurrection Plant Craterostigma Plantagineum

Bartels, Dorothea; Schneider, Katharina; Piatkowski, Detlef; Elster, Ralph; Iturriaga, Gabriel; Terstappen, Georg C.; Bình, Lê Trần; Salamini, F.

doi:10.1007/978-1-4615-3304-7_66

Cited by 4 publications

(5 citation statements)

References 18 publications

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“…An attractive feature of Craterostigma plantagineum is the fact that desiccation tolerance can be studied both in the whole plant and in undifferentiated callus cultures. Desiccation-tolerant callus is obtained by treating the callus with exogenous abscisic acid (ABA) prior to drying (Bartels et al 1991b). The dehydration process and ABA treatment lead to a massive synthesis of new transcripts and proteins.…”

Section: Desiccation Tolerance In Model Plantsmentioning

confidence: 99%

Approaches to improve stress tolerance using molecular genetics

Bartels¹,

Nelson

1994

Plant Cell & Environment

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147

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Plant productivity is greatly affected by environmental stress factors. In this review, we give an overview of molecular approaches that have been taken to study stress tolerance; in particular, we consider drought, salt and cold stress. Strategies and perspectives in using molecular biology to improve stress tolerance are outlined describing specific examples. Osmotic stress is associated with the synthesis of novel polypeptides and/or osmolytes. The spectrum and action of these different metabolites are summarized. A number of stress‐related genes have been characterized and several representatives are discussed. After transforming plants with these genes, the effect of the encoded proteins on altered stress behaviour is examined. If genes with enzymatic functions were chosen for these experiments, complete pathways may be altered and this can implement the production of novel metabolites conferring stress tolerance.

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Section: Desiccation Tolerance In Model Plantsmentioning

confidence: 99%

Approaches to improve stress tolerance using molecular genetics

Bartels¹,

Nelson

1994

Plant Cell & Environment

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147

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“…3) to the Atmyb2 gene fromArabidopsis which may be involved in transducing the drought stress signal mediated by ABA [59]. ABA mediates desiccation tolerance during embryo maturation in several plants [7,8,17,57], in vegetative and callus tissue of Craterostigma [4,5], and is also involved in the response to different abiotic stresses such as salt, cold and wound [8,17]. The results presented in this work revealed that ABA and desiccation up-modulate transcription of specific myb genes in Craterostigma (Fig.…”

Section: Gene Expression Pattern Ofcpm Genesmentioning

confidence: 63%

“…We have reported before [4,5] that pretreatment with ABA is required to induce desiccation tolerance of Craterostigma callus tissue. Under these conditions, several genes are induced at the transcriptional level but none of them are induced by dehydration alone.…”

Section: Gene Expression Pattern Ofcpm Genesmentioning

confidence: 99%

“…This resurrection plant can withstand complete dehydration for long periods and resumes its normal shape and physiology upon rehydration. Several genes induced by drought from Craterostigma have been isolated and sequenced [4,5,48]. As an initial step towards characterizing transcription factor genes involved in desiccation tolerance from Craterostigma, its myb genes are being studied.…”

Section: Introductionmentioning

confidence: 99%

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A family of novel myb-related genes from the resurrection plant Craterostigma plantagineum are specifically expressed in callus and roots in response to ABA or desiccation

Iturriaga¹,

Leyns²,

Villegas³

et al. 1996

Plant Mol Biol

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A cDNA and two genomic clones comprising highly similar genes that encode a protein with a Myb-related DNA-binding domain were isolated from the resurrection plant Craterostigma plantagineum. The structure of cpm5 and cpm10 (Craterostigma plantagineum myb) genes consists of three putative exons encoding a protein of 36.6 kDa. The cDNA of cpm7 encodes a closely related protein of 36.8 kDa. The canonical Myb domain present in transcriptional activators of yeast, animals and plants was localized in the amino terminus of deduced Cpm5, Cpm7 and Cpm10 proteins and corresponds to the two Myb repeats found in plants. The Myb domain of Cpm deduced proteins and a short stretch of amino acids adjacent to this region are closely related to a myb gene from Arabidopsis thaliana which is expressed in response to osmotic stress and ABA. The rest of the deduced protein has no similarity to other reported sequences. The myb-related genes in the Craterostigma genome comprise a small gene family of 6-8 members as estimated by hybridization with a bona fide Myb domain probe. Northern blot experiments showed specific expression of cpm10 in undifferentiated callus tissue up-modulated by ABA and expression of cpm7 mRNA in roots up-regulated by dehydration.

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“…As a representative of the resurrection plants we have chosen Craterostigma plantagineum for the isolation of genes related to desiccation tolerance [3,35]. Desiccation tolerance is exhibited in both vegetative plant tissues and undifferentiated ABA-treated callus tissue of C. plantagineum [2].…”

Section: Introductionmentioning

confidence: 99%

Structure and regulation of an ABA- and desiccation-responsive gene from the resurrection plant Craterostigma plantagineum

Michel¹,

Furini²,

Salamini³

et al. 1994

Plant Mol Biol

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A gene from the resurrection plant Craterostigma plantagineum (CDeT6-19) encoding a protein with sequence similarity to a major group of late embryogenesis-abundant proteins (termed rab17, dehydrin or Lea D11) is regulated by abscisic acid (ABA) and desiccation. The corresponding transcript and protein is highly inducible in vegetative and callus tissue. To analyse the mechanism of CDeT6-19 regulation its promoter was fused to the beta-glucuronidase reporter gene (GUS) and introduced by PEG (polyethylene glycol) into protoplasts of Craterostigma or tobacco. With 889 bp of promoter sequence the GUS expression was significantly stimulated by ABA treatment in transient expression assays. ABA responsiveness was still observed with shorter promoter fragments, although they gave rise to lower GUS activities. Sequence comparisons with promoters from related genes of other species identified the conservation of potential ABA-responsive elements. In tobacco and Craterostigma plants stably transformed with CDeT6-19 promoter constructs a basal GUS activity is observed. However, GUS expression is enhanced by ABA or drying treatment of leaf tissues. In tobacco high promoter activity was observed in mature seeds (embryos) and in pollen.

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Molecular Analysis of Desiccation Tolerance in the Resurrection Plant Craterostigma Plantagineum

Cited by 4 publications

References 18 publications

Approaches to improve stress tolerance using molecular genetics

Approaches to improve stress tolerance using molecular genetics

A family of novel myb-related genes from the resurrection plant Craterostigma plantagineum are specifically expressed in callus and roots in response to ABA or desiccation

Structure and regulation of an ABA- and desiccation-responsive gene from the resurrection plant Craterostigma plantagineum

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