A family of novel myb-related genes from the resurrection plant Craterostigma plantagineum are specifically expressed in callus and roots in response to ABA or desiccation

Iturriaga, Gabriel; Leyns, Luc; Villegas, Antonio; Gharaibeh, Rima; Salamini, F.; Bartels, Dorothea

doi:10.1007/bf00020211

Cited by 55 publications

(35 citation statements)

References 72 publications

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“…Genes responsive to ABA usually contain ABA-responsive elements consisting of the (C/T)ACGTGGC consensus sequence and are transactivated by bZIP transcription factors (Choi et al, 2000). Three classes of transcription factors have been characterized in Craterostigma: a heat shock transcription factor (Bockel et al, 1998), two members of the homeodomain Leu zipper family (Frank et al, 1998), and three MYB genes (Iturriaga et al, 1996). Here we show that Arabidopsis transgenic lines overexpressing the heterologous MYB transcription factor gene CpMYB10 are stress tolerant, Glcinsensitive, and ABA hypersensitive.…”

mentioning

confidence: 80%

“…We have previously reported the cloning of a cDNA and two genomic MYB genes from Craterostigma (Iturriaga et al, 1996). The CpMYB7 cDNA and CpMYB5 and CpMYB10 genomic clones share between them 96% to 98% identity and have the canonical MYB DNA-binding domain with the R2R3-type structure that is predominant in plants (Stracke et al, 2001).…”

Section: Cpmyb10mentioning

confidence: 99%

“…In Arabidopsis, the closest homolog to these MYB genes from Craterostigma is AtMYB2, which is induced by ABA, dehydration, and salt stresses (Urao et al, 1993). Although the DNA-binding domain of CpMYB10 and AtMYB2 shares 92% similarity, the 230-amino acid C terminus of CpMYB10 is different from the corresponding AtMYB2 region, except for a stretch of 31 amino acids that shows 87% similarity (Iturriaga et al, 1996).…”

Section: Cpmyb10mentioning

confidence: 99%

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Stress Tolerance and Glucose Insensitive Phenotypes in Arabidopsis Overexpressing theCpMYB10Transcription Factor Gene

Villalobos¹,

Bartels²,

Iturriaga³

2004

Plant Physiology

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The resurrection plant Craterostigma plantagineum has the ability to survive complete dehydration. In an attempt to further understand desiccation tolerance in this plant, the CpMYB10 transcription factor gene was functionally characterized. CpMYB10 is rapidly induced by dehydration and abscisic acid (ABA) treatments in leaves and roots, but no expression was detected in fully hydrated tissues. Electrophoretic mobility shift assay experiments showed binding of rCpMYB10 to specific mybRE elements within the LEA Cp11-24 and CpMYB10 promoters. Localization of CpMYB10 transcript by in situ reverse transcription-PCR reactions showed expression in vascular tissues, parenchyma, and epidermis both in leaves and roots in response to ABA. Transgenic Arabidopsis plants transformed with CpMYB10 promoter fused to GUS gene showed reporter expression under ABA and stress conditions in several organs. Overexpression of CpMYB10 cDNA in Arabidopsis led to desiccation and salt tolerance of transgenics lines. Interestingly, it was found that plants overexpressing CpMYB10 exhibited Glc-insensitive and ABA hypersensitive phenotypes. Therefore, our results indicate that CpMYB10 in Arabidopsis is mediating stress tolerance and altering ABA and Glc signaling responses.

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confidence: 80%

Section: Cpmyb10mentioning

confidence: 99%

Section: Cpmyb10mentioning

confidence: 99%

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Stress Tolerance and Glucose Insensitive Phenotypes in Arabidopsis Overexpressing theCpMYB10Transcription Factor Gene

Villalobos¹,

Bartels²,

Iturriaga³

2004

Plant Physiology

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“…In the past decade, the R2R3-MYB genes have been extensively studied and members of the MYB family have been found to be involved in a variety of biological functions like phenylpropanoid metabolism (Grotewold et al 1994;Hichri et al 2011), biotic and abiotic stress (Segarra et al 2009;Lippold et al 2009), cell shape such as Am MIXTA (Noda et al 1994), differentiation (Oppenheimer et al 1991;Kang et al 2009;Xie et al 2010), hormone responses i.e. AtMYB2 (Urao et al 1993), GAMYB and CpMYB (Gubler et al 1995 ;Iturriaga et al 1996), formation of B-type cyclin (Ito et al 2001) or during plant defense reactions i.e. NtMYB1 (Yang and Klessig 1996;Liu et al 2008).…”

Section: Functions Of Mybmentioning

confidence: 99%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

822

544

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Regulation of gene expression at the level of transcription controls many crucial biological processes. Transcription factors (TFs) play a great role in controlling cellular processes and MYB TF family is large and involved in controlling various processes like responses to biotic and abiotic stresses, development, differentiation, metabolism, defense etc. Here, we review MYB TFs with particular emphasis on their role in controlling different biological processes. This will provide valuable insights in understanding regulatory networks and associated functions to develop strategies for crop improvement.

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“…Some plant R2R3 MYB genes are known to regulate secondary metabolism, particularly in the phenylpropanoid pathway (Paz-Ares, 1987;Grotewold et al, 1994;Sablowski et al, 1994;Moyano et al, 1996;Tamagnone et al, 1998) and tryptophan biosynthesis (Bender and Fink, 1998). Others contribute to processes of cellular morphogenesis (Oppenheimer et al, 1991;Noda et al, 1994;Waites et al, 1998), signal transduction in plant growth (Gubler et al, 1995;Iturriaga et al, 1996), abiotic stress (Urao et al, 1993;Magaraggia, 1997;Hoeren et al, 1998), and pathogen defense (Yang and Klessig, 1996). Nevertheless, very little is known about the functions of the majority of R2R3 MYB transcription factors in Arabidopsis.…”

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Function Search in a Large Transcription Factor Gene Family in Arabidopsis: Assessing the Potential of Reverse Genetics to Identify Insertional Mutations in R2R3 MYB Genes

et al. 1999

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More than 92 genes encoding MYB transcription factors of the R2R3 class have been described in Arabidopsis. The functions of a few members of this large gene family have been described, indicating important roles for R2R3 MYB transcription factors in the regulation of secondary metabolism, cell shape, and disease resistance, and in responses to growth regulators and stresses. For the majority of the genes in this family, however, little functional information is available. As the first step to characterizing these genes functionally, the sequences of Ͼ 90 family members, and the map positions and expression profiles of Ͼ 60 members, have been determined previously. An important second step in the functional analysis of the MYB family, through a process of reverse genetics that entails the isolation of insertion mutants, is described here. For this purpose, a variety of gene disruption resources has been used, including T-DNAinsertion populations and three distinct populations that harbor transposon insertions. We report the isolation of 47 insertions into 36 distinct MYB genes by screening a total of 73 genes. These defined insertion lines will provide the foundation for subsequent detailed functional analyses for the assignment of specific functions to individual members of the R2R3 MYB gene family. INTRODUCTIONThe assignment of biological function to the large number of genes that have now been sequenced, with new sequence data being compiled rapidly, is currently one of the most challenging goals in biology. Genetic analysis, particularly the effects of loss-of-function mutations, is of central importance to achieving this goal. In plants, unlike yeast, targeted gene disruption is laborious and inefficient (Kempin et al., 1997). Gene silencing by antisense or sense suppression is also a common approach to studying plant gene function (Kooter and Mol, 1993;Baulcombe, 1996), but the specificity and extent of gene disruption through such methods have not been extensively tested, 1 These authors contributed equally to this work. 2 Current address: CPRO-DLO, Department of Molecular Biology, Droevendaalsesteeg 1, 6700 AA Wageningen, The Netherlands. 3 Current address: Laboratoire de Radiobiologie Vegetale DEVM, CEA, Cadarache, 13108 St. Paul-lez Durance Cedex, France. 4 To whom correspondence should be addressed. E-mail bevan@ bbsrc.ac.uk; fax 44-1603-505725. 1828The Plant Cell so the analysis and interpretation of suppression sometimes have proved difficult (van der Krol et al., 1990;Höfgen et al., 1994).T-DNA and transposable elements can alter gene function upon insertion into coding or regulatory sequences (Feldmann, 1991;Martienssen, 1998). Many lines of Arabidopsis harboring either T-DNA or transposon insertions have been generated (Koncz et al., 1992;Azpiroz-Leehan and Feldmann, 1997;Bouchez and Höfte, 1998;Martienssen, 1998;Wisman et al., 1998aWisman et al., , 1998b. Individual lines carrying insertions within a gene of interest can be identified by polymerase chain reaction (PCR) by using a gene-sp...

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A family of novel myb-related genes from the resurrection plant Craterostigma plantagineum are specifically expressed in callus and roots in response to ABA or desiccation

Cited by 55 publications

References 72 publications

Stress Tolerance and Glucose Insensitive Phenotypes in Arabidopsis Overexpressing theCpMYB10Transcription Factor Gene

Stress Tolerance and Glucose Insensitive Phenotypes in Arabidopsis Overexpressing theCpMYB10Transcription Factor Gene

MYB transcription factor genes as regulators for plant responses: an overview

Function Search in a Large Transcription Factor Gene Family in Arabidopsis: Assessing the Potential of Reverse Genetics to Identify Insertional Mutations in R2R3 MYB Genes

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