Molecular Analysis in the differentiation of Colletotrichum gloeosporioides isolates from the cashew and mango trees

Serra, Ilka Márcia Ribeiro de Souza; Menezes, Maria; Coelho, Rildo Sartori Barbosa; Ferraz, Gabriela Moraes Guerra; Montarroyos, Angélica Virgínia Valois; Martins, Luiza Suely Semem

doi:10.1590/s1516-89132011000600004

Cited by 21 publications

(16 citation statements)

References 17 publications

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“…The mycelial growth has been reported to be a criterion that helps to differentiate the species of Colletotrichum (Waller et al, 1993;Crouch et al, 2009;Liu et al, 2012). However, according to Serra et al (2006).Although it is not a stable criterion of differentiation of Colletotrichum species, but it plays a significant role in variability within the species. Bailey et al (1995) argued that many isolates of Colletotrichum often show extensive variation in culture and furthermore, the culture conditions , including the media,the age of culture and the temperatures used, cannot be standard betweenlaboratories (Sutton 1992).…”

Section: Discussionmentioning

confidence: 99%

Cultural and morphological variations of Colletotrichum spp associated with anthracnose of various fruits in Cameroon

Kamdoum¹,

Noumbo²,

Jules-Roger³

2016

IJEAB

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Section: Discussionmentioning

confidence: 99%

Cultural and morphological variations of Colletotrichum spp associated with anthracnose of various fruits in Cameroon

Kamdoum¹,

Noumbo²,

Jules-Roger³

2016

IJEAB

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“…Posteriormente, fragmentos do micélio de colônias características do gênero Colletotrichum, foram transferidos para tubos contendo BDA e incubados nas mesmas condições já citadas. Após a confirmação da espécie, através da análise da seqüência de ITS do DNA ribosossomal, de acordo com Serra et al (21), o isolado foi codificado com sigla e as culturas puras foram preservadas em tubos de ensaio contendo BDA, sob refrigeração (5 0 C).…”

Section: Materiais E Métodosunclassified

Efeito de óleos essenciais como alternativa no controle de Colletotrichum gloeosporioides, em pimenta

2012

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O fungo C. gloeosporioides foi inoculado, através de ferimento, logo após a imersão dos frutos. As avaliações foram realizadas diariamente através de medição do diâmetro das colônias e das lesões, tomando-se duas medições em sentidos diametralmente opostos. Pode-se observar que no experimento in vitro todos os óleos, com exceção dos óleos de babaçu, semente de uva e a mêndoa , tivera m excelentes resulta dos inibindo o crescimento do fungo. No resultado obtido em pós-colheita foi observado que apenas o óleo de babaçu não foi eficiente em reduzir o desenvolvimento da lesão de antracnose. Dados relevantes foram observados pa ra os óleos de semente de u va e a mêndoa , qu e nã o apresentaram efeito direto sobre o fungo in vitro, porém no tratamento pós-colheita apresentaram bons resultados, reduzindo a lesão causada por C. gloeosporioides, sugerindo assim que estes óleos possam ser utilizados como indu tores de resistência em frutos de pimenta com a ntracnose.Colle to tric hu m spp. ca uses the disease k nown a s a nthracnose. Alternative methods that are efficient and less aggressive have been widely tested. Among these, there is interest in the scientific community by the u se of essentia l oils extra cted from plants. The subject of the present study was to evaluate the effect of essential oils eucalyptus, copaiba, andiroba, babassu, coconut, neem, grape seed, a lmond, mint a nd pa u rosa in different concentra tions on the development of Co lletotrichum gloeosporioide s, "in vitro " a nd in fru its of pepper in post ha rvest. T he experiment in vitro wa s performed using five concentrations (0.2, 0.4, 0.6, 0.8 and 1.0%) of the ten oils mixed with BDA. The varia bles were the radial growth rate. The test was done post-harvest immersion of the fruits of pepper for 5 minutes, the same oils used in the previous experiment, using Sousa, R.M.S; Serra, I.M.R.S; Melo, T.A. Effect of essential oils as an alternative in the control of Colletotrichum gloesporioides in pepper. Summa Phytopathologica, v.38, n.1, p.42-47, 2012. the highest concentra tion. T he fu ngu s C. g lo e o sp o rio id e s wa s inoculated through wounds shortly after the immersion of the fruit. The evaluations were performed daily by measuring the diameter of the colonies and injuries, taking measurements at two diametrically opposite directions. It can be observed in experiment in vitro all oils, except for babaçu oil, grape seed and almond, excellent results were inhibiting the growth of fu ngus. T he results obtained in post-harvest was observed that only the babaçu oil was not effective in reducing lesion development of anthracnose. Relevant data were observed for oils of grape seed and almond, which had no direct effect on the fungus in vitro, but in post-harvest treatment showed good results, reducing the damage caused by C. gloeosporioides, suggesting that these oils can be used to indu ce resistance in pepper fruit with a nthracnose.Keywords: antifungal effect, induced resistance, anthracnose. ABSTRACTO fungo Colletotrichum spp. causa a doe...

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“…Identification of C. gloeosporioides based on cultural, mycelial growth rate and morphological characteristics can be confused with other species within the genus, more especially C. acutatum (Chowdappa and Kumar, 2012;Reddy, 2015). According to Serra et al (2011), different species of Colletotrichum are capable of infecting a single host. The foliage infection of C. acutatum and C. gloeosporioides are difficult to differentiate in terms of their symptoms and cultural morphology (Shi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The foliage infection of C. acutatum and C. gloeosporioides are difficult to differentiate in terms of their symptoms and cultural morphology (Shi et al, 2008). This makes it difficult to differentiate between C. acutatum and C. gloeosporioides; hence the need to use molecular techniques for the proper identification of Colletotrichum isolates (Serra et al, 2011). The polymerase chain reaction (PCR) technique has been documented as one means of properly identifying C. gloeosporioides isolates (Shi et al, 2008;Serra et al, 2011;Raj et al, 2013;Chagas et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular characterisation of Colletotrichum gloeosporioides (Penz) isolates obtained from Dioscorea rotundata (Poir)

Kwodaga¹,

Sowley²,

Badii³

2020

Afr. J. Biotechnol.

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Anthracnose disease is a major constraint to yam production in tropical West Africa and anywhere the crop is cultivated. This study determined the cultural characteristics and growth rates of mycelia and also characterised 6 isolates of Colletotrichum gloeosporioides, the causal agent of the yam anthracnose disease, obtained from Dioscorea rotundata leaves, vines and setts in the Tolon District of Ghana. The cultural characteristics and mycelial growth rates of the isolates were determined on Potato Dextrose Agar (PDA). The C. gloeosporioides isolates were characterised using polymerase chain reaction technique with the universal primer pairs ITS1/ITS4 and NS1/NS2, C. gloeosporioides species specific primer pairs CgInt/ITS4 and CgLac-f/CgLac-r, and C. acutatum species specific primer pairs CaGlu-f1/CaGlu-r1 and Ca-f1/Ca-r1. Based on the PCR, six isolates of C. gloeosporioides with distinct cultural characteristics were obtained. There were no significant differences (P ≤ 0.05) in mycelial growth rates among the isolates. The C. gloeosporioides isolates produced characteristic band sizes on ITS1/ITS4, NS1/NS2, CgInt/ITS4 and CgLac-f/CgLac-r. None of the isolates produced a band on CaGlu-f1/CaGlu-r1 and Ca-f1/Ca-r1. The proper identification of C. gloeosporioides, the pathogen responsible for the D. rotundata anthracnose is important for the proper management of the disease.

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Molecular Analysis in the differentiation of Colletotrichum gloeosporioides isolates from the cashew and mango trees

Abstract: ABSTRACT

Cited by 21 publications

References 17 publications

Cultural and morphological variations of Colletotrichum spp associated with anthracnose of various fruits in Cameroon

Cultural and morphological variations of Colletotrichum spp associated with anthracnose of various fruits in Cameroon

Efeito de óleos essenciais como alternativa no controle de Colletotrichum gloeosporioides, em pimenta

Morphological and molecular characterisation of Colletotrichum gloeosporioides (Penz) isolates obtained from Dioscorea rotundata (Poir)

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