Cultural and morphological variations of Colletotrichum spp associated with anthracnose of various fruits in Cameroon

Kamdoum, Elie Keuete; Noumbo, G. R. Tsopmbeng; Jules-Roger, Kuiate

doi:10.22161/ijeab/1.4.48

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…The resulting conidial suspension was was stirred with a vortex to release the conidia (macroconidia and microconidia) from the conidiophores, then this suspension was filtered (to remove mycelial fragments) and a drop of Tween 20 was added to homogenize the suspension. Finally the number of conidia/ml was counted using a Thoma cell [17]. The percentage of sporulation inhibition was determined by the following formula:…”

Section: Isolation Of Fusarium Spp Associated With Dry Rot Of Potato ...mentioning

confidence: 99%

Antifungal Activity of Some Inorganic Salts on the Development of Fusarium spp., Causal Agents of Potato Tuber Dry Rot

Lionelle,

Elie,

Gaston

2023

AJAAR

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Potato dry rot caused by Fusarium spp. is one of the main fungal diseases hampering the production and marketing of potato tubers in Cameroon. This study was carried out in order to improve the health status of potato tubers and their preservation. To achieve this objective, Fusarium spp. were isolated on the Potato Dextrose Agar (PDA) culture medium and identified on the basis of morphological and microscopic characteristics using reference documents. Antifungal activity of six inorganic salts was evaluated in vitro on the development of Fusarium spp. and on potato tubers artificially infected with these fungal species. The results showed that all the inorganic salts had a depressant effect on the development of Fusarium spp. Copper sulfate inhibited at 100%, the radial growth and sporulation of Fusarium spp. at all concentrations. Sodium metabisulfite and sodium carbonate inhibited at 100%, the growth and sporulation of all fungal species from a concentration of 6.4 mg/ml. No lesions were observed on inoculated potato tubers when they were treated with copper sulfate. These results suggest the implementation of a strategy to control dry rot of potato tubers through the use of inorganic salts, in order to reduce the losses caused by Fusarium spp.

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Section: Isolation Of Fusarium Spp Associated With Dry Rot Of Potato ...mentioning

confidence: 99%

Antifungal Activity of Some Inorganic Salts on the Development of Fusarium spp., Causal Agents of Potato Tuber Dry Rot

Lionelle,

Elie,

Gaston

2023

AJAAR

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“…The activity of the plant extracts (aqueous and ethanolic) was evaluated at 7.5 mg/ml and 15 mg/ml on the radial growth of Fusarium oxysporum f. sp. cubense isolates on PDA at pH6 using the poisoning solid medium method (Keuete et al, 2016;Yaouba et al, 2017). In fact, after solidification of the medium, a 5 mm in diameter mycelial explant was removed using a cookie cutter, and aseptically placed in the center of each Petri dish supplemented with plant extracts.…”

Section: Antifungal Activity Of Plant Extractsmentioning

confidence: 99%

Cultural Characterization of Five Isolates of Fusarium Oxysporum F. Sp. Cubense (Banana Fusarium Wilt) and Antifungal Activity of Plant Extracts

Djeugap,

Abireche,

Donfack

et al. 2023

Pak. J. Phytopathol.

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Plantain (Musa spp.) is one of the most important food crops in Cameroon and elsewhere in Central and West Africa. However, its production is threatened by many diseases, including banana Fusarium wilt. The objective of this work was to contribute to the improvement of plantains production through the assessment of the behavior of the pathogen isolates to some environmental factors and the biological management of Fusarium wilt in Lab conditions. To achieve this, roots and leaves samples were collected from infected plantain plantations in five divisions of the West Region of Cameroon and brought to the laboratory for isolation and identification of the pathogen. Then, the pathogenicity test was carried out and the optimal growth conditions of Fusarium oxysporum f. sp. cubense (FOC), were evaluated on three culture media (V8, PDA and Banana Leaf Extract Dextrose Agar), five pH (5, 6, 7, 8 and 9) and two temperatures (18 and 25°C). Finally, antifungal activities of aqueous and ethanolic extracts of Callistemon viminalis and Chenopodium ambrosioides were evaluated on the growth of the five isolates (FOC-DSC, FOC-BAL, FOC-FBT, FOC-BAN and FOC-KEK) at 7.5 mg/mL and 15mg/mL. The results showed that all the five isolates were pathogenic. However, the isolates from Foumbot (FOC-FBT) and Kekem (FOC-KEK) were more aggressive. The V8 and BLEDA media at the pH 6 and 25°C were more suitable for the growth of all the isolates of FOC. The efficiency of ethanolic extract of C. ambrosioides was significantly comparable to the chemical fungicide (Eagrow Care) at 15mg/mL. Isolates of the Fusarium wilt pathogen in the West Region of Cameroon are phenotypically different and due to the efficiency of the ethanolic extract of C. ambrosioides, it may be exploit for its antifungal properties on the management of the disease.

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Identification of Colletotrichum Species Associated with Chili Anthracnose in Indonesia by Morphological Characteristics and Species-Specific Primers

Widodo¹,

Hidayat²

2017

Asian J. of Plant Pathology

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Cultural and morphological variations of Colletotrichum spp associated with anthracnose of various fruits in Cameroon

Abstract: Abstract-

Cited by 6 publications

References 16 publications

Antifungal Activity of Some Inorganic Salts on the Development of Fusarium spp., Causal Agents of Potato Tuber Dry Rot

Antifungal Activity of Some Inorganic Salts on the Development of Fusarium spp., Causal Agents of Potato Tuber Dry Rot

Cultural Characterization of Five Isolates of Fusarium Oxysporum F. Sp. Cubense (Banana Fusarium Wilt) and Antifungal Activity of Plant Extracts

Identification of Colletotrichum Species Associated with Chili Anthracnose in Indonesia by Morphological Characteristics and Species-Specific Primers

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