Molecular analysis and antimicrobial susceptibility of enterotoxigenic Escherichia coli from diarrheal patients

Pan, Hongyu; Zhang, Jianmin; Kuang, Dai; Yang, Xiaowei; Ju, Wenting; Huang, Zheng; Guo, Jiayin; Li, Yong; Zhang, Pingping; Shi, Weimin; Jin, Huang; Shi, Xianming; Xu, Xuebin; Meng, Jianghong

doi:10.1016/j.diagmicrobio.2014.10.008

Cited by 9 publications

(7 citation statements)

References 27 publications

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“…CS21 was the most prevalent CF (48%) in Shenzhen isolates, followed by CS6 (34%). Similar virulence factor profile was also observed in ETEC isolates from diarrheal cases in Shanghai, China (ST: 74%, CS21: 63%, CS6: 41%) (Pan et al, 2015). In contrast, a systematic review showed that globally, ST-positive, LT-positive, and ST + LTpositive ETEC accounted for 50, 25, and 27% non-travel human infections, respectively, and the most prevalent CFs were CFA/I, followed by CS21 and CS6 (Isidean et al, 2011).…”

Section: Discussionsupporting

confidence: 59%

“…Despite the high prevalence in children and travelers, ETEC also lead to substantial infections in adults in endemic areas (Lamberti et al, 2014). Moreover, it is intriguing that most ETEC infections in Shenzhen (Li et al, 2017), as well as several other developed cities and province in China Qu et al, 2014;Pan et al, 2015), such as Beijing and Shanghai, were from indigenous adults. However, there is currently a lack of understanding on the epidemiology of ETEC infections in indigenous adults.…”

Section: Discussionmentioning

confidence: 99%

“…L11 can be detected in children aged <5 years and adult travelers from multiple countries, but at a low fraction (4%) in the global dataset (von Mentzer et al, 2014). If taken serotype and MLST-ST into consideration, L11 (O159:H34, ST218) isolates was only identified in local diarrheal cases of Korea (Chung et al, 2019) and China Pan et al, 2015;Li et al, 2017), and the virulence factor profiles (STh+, without known CS) of isolates from two countries were identical, indicating the possibility of recent transmission. L2/4 was a previously undefined lineage, which located between L2 and L4 in the phylogenic tree.…”

Section: Discussionmentioning

confidence: 99%

“…However, no CF was reported in Danish isolates whereas all Shenzhen isolates carried CS21. L4 lineage was also detected at low fraction (6%) in the global dataset (von Mentzer et al, 2014), and the subtyping combination of Shenzhen isolates, i.e., L4 (O25:H16, ST1491), was only identified in isolates from few travelers returning to the UK (Boxall et al, 2020) and from local diarrheal cases of China Pan et al, 2015;Li et al, 2017). Taken together, these results revealed that the major circulating ETEC lineages in Shenzhen, as well as in several other developed regions of China were distinctive, which are not the commonly detected global or endemic lineages.…”

Section: Discussionmentioning

confidence: 99%

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Genomic Epidemiology and Antimicrobial Susceptibility Profile of Enterotoxigenic Escherichia coli From Outpatients With Diarrhea in Shenzhen, China, 2015–2020

Yang

Zuo

et al. 2021

Front. Microbiol.

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Enterotoxigenic Escherichia coli (ETEC) is the leading cause of severe diarrhea in children and the most common cause of diarrhea in travelers. However, most ETEC infections in Shenzhen, China were from indigenous adults. In this study, we characterized 106 ETEC isolates from indigenous outpatients with diarrhea (77% were adults aged >20 years) in Shenzhen between 2015 and 2020 by whole-genome sequencing and antimicrobial susceptibility testing. Shenzhen ETEC isolates showed a remarkable high diversity, which belonged to four E. coli phylogroups (A: 71%, B1: 13%, E: 10%, and D: 6%) and 15 ETEC lineages, with L11 (25%, O159:H34/O159:H43, ST218/ST3153), novel L2/4 (21%, O6:H16, ST48), and L4 (15%, O25:H16, ST1491) being major lineages. Heat-stable toxin (ST) was most prevalent (76%, STh: 60% STp: 16%), followed by heat-labile toxin (LT, 17%) and ST + LT (7%). One or multiple colonization factors (CFs) were identified in 68 (64%) isolates, with the common CFs being CS21 (48%) and CS6 (34%). Antimicrobial resistance mutation/gene profiles of genomes were concordant with the phenotype testing results of 52 representative isolates, which revealed high resistance rate to nalidixic acid (71%), ampicillin (69%), and ampicillin/sulbactam (46%), and demonstrated that the novel L2/4 was a multidrug-resistant lineage. This study provides novel insight into the genomic epidemiology and antimicrobial susceptibility profile of ETEC infections in indigenous adults for the first time, which further improves our understanding on ETEC epidemiology and has implications for the development of vaccine and future surveillance and prevention of ETEC infections.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genomic Epidemiology and Antimicrobial Susceptibility Profile of Enterotoxigenic Escherichia coli From Outpatients With Diarrhea in Shenzhen, China, 2015–2020

Yang

Zuo

et al. 2021

Front. Microbiol.

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“…Enterotoxigenic Escherichia coli (ETEC) is a common cause of childhood diarrhea in resource-limited regions and diarrhea in adult travelers to these areas 1 2 . ETEC is also an important cause of diarrhea in piglets 3 .…”

mentioning

confidence: 99%

Proteome analysis for the global proteins in the jejunum tissues of enterotoxigenic Escherichia coli -infected piglets

Ren

Yin

Chen

et al. 2016

Sci Rep

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Enterotoxigenic Escherichia coli (ETEC) is a common cause of diarrhea in humans and livestock. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) combined with multidimensional liquid chromatography (LC) and MS analysis was used for screening the differentially expressed proteins in piglet jejunum after ETEC infection. Totally 1,897 proteins were identified with quantitative information in piglet jejunum. We identified 92 differentially expressed proteins in ETEC-induced diarrhea, of which 30 were up regulated and 62 down regulated. Most of the differentially expressed proteins were involved in intestinal function of binding, metabolic process, catalytic activity and immune responses. The inhibition of intestinal immune responses in the jejunum in ETEC-induced diarrhea was also validated by immunobloting and RT-PCR. Our study is the first attempt to analyze the protein profile of ETEC-infected piglets by quantitative proteomics, and our findings could provide valuable information with respect to better understanding the host response to ETEC infection.

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Detection and genetic analysis of Escherichia coli from Tonle Sap Lake and its tributaries in Cambodia: Spatial distribution, seasonal variation, pathogenicity, and antimicrobial resistance

YONEDA

Rozanah²,

Nishiyama³

et al. 2022

Environmental Pollution

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Molecular analysis and antimicrobial susceptibility of enterotoxigenic Escherichia coli from diarrheal patients

Cited by 9 publications

References 27 publications

Genomic Epidemiology and Antimicrobial Susceptibility Profile of Enterotoxigenic Escherichia coli From Outpatients With Diarrhea in Shenzhen, China, 2015–2020

Genomic Epidemiology and Antimicrobial Susceptibility Profile of Enterotoxigenic Escherichia coli From Outpatients With Diarrhea in Shenzhen, China, 2015–2020

Proteome analysis for the global proteins in the jejunum tissues of enterotoxigenic Escherichia coli -infected piglets

Detection and genetic analysis of Escherichia coli from Tonle Sap Lake and its tributaries in Cambodia: Spatial distribution, seasonal variation, pathogenicity, and antimicrobial resistance

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