Proteome analysis for the global proteins in the jejunum tissues of enterotoxigenic Escherichia coli -infected piglets

Ren, Wenkai; Yin, Jie; Chen, Shuai; Duan, Jielin; Liu, Gang; Li, Tiejun; Li, Nengzhang; Peng, Yuanyi; Tan, Bin; Yin, Yulong

doi:10.1038/srep25640

Cited by 30 publications

(39 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study has shown that ETEC infection inhibits the activation of NF-κB and mitogen-activated protein kinases pathways in the jejunum (20), indicating that new pathways are associated with expression of IL-17 during ETEC infection. Previous proteomic analysis indicated that ETEC infection upregulates ribosomal protein S6 kinase in the piglet jejunum (20), which suggests that ETEC infection may activate mTORC1 signaling.…”

Section: Resultsmentioning

confidence: 99%

“…Previous proteomic analysis indicated that ETEC infection upregulates ribosomal protein S6 kinase in the piglet jejunum (20), which suggests that ETEC infection may activate mTORC1 signaling. The mTORC1 has a critical role in Th17 responses and in IL-17 expression (26).…”

Section: Resultsmentioning

confidence: 99%

“…Piglets (Landrace Yorkshire; 18 days old) were purchased from ZhengDa Co., Chongqing, China. ETEC infection in piglets was established according to previous reports (19, 20). The jejunum samples were collected.…”

Section: Methodsmentioning

confidence: 99%

“…Real-time PCR was performed according to our previous study (20, 24). Primers (Table S1 in Supplementary Material) were selected according to previous references.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoblotting was performed according to our previous study (20, 24). Signal intensity was digitally quantified and normalized to actin or PCNA protein abundance.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Intestinal Microbiota-Derived GABA Mediates Interleukin-17 Expression during Enterotoxigenic Escherichia coli Infection

et al. 2017

Self Cite

View full text Add to dashboard Cite

Intestinal microbiota has critical importance in pathogenesis of intestinal infection; however, the role of intestinal microbiota in intestinal immunity during enterotoxigenic Escherichia coli (ETEC) infection is poorly understood. The present study tested the hypothesis that the intestinal microbiota is associated with intestinal interleukin-17 (IL-17) expression in response to ETEC infection. Here, we found ETEC infection induced expression of intestinal IL-17 and dysbiosis of intestinal microbiota, increasing abundance of γ-aminobutyric acid (GABA)-producing Lactococcus lactis subsp. lactis. Antibiotics treatment in mice lowered the expression of intestinal IL-17 during ETEC infection, while GABA or L. lactis subsp. lactis administration restored the expression of intestinal IL-17. L. lactis subsp. lactis administration also promoted expression of intestinal IL-17 in germ-free mice during ETEC infection. GABA enhanced intestinal IL-17 expression in the context of ETEC infection through activating mechanistic target of rapamycin complex 1 (mTORC1)-ribosomal protein S6 kinase 1 (S6K1) signaling. GABA–mTORC1 signaling also affected intestinal IL-17 expression in response to Citrobacter rodentium infection and in drug-induced model of intestinal inflammation. These findings highlight the importance of intestinal GABA signaling in intestinal IL-17 expression during intestinal infection and indicate the potential of intestinal microbiota-GABA signaling in IL-17-associated intestinal diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Real-time PCR was performed according to our previous study (20, 24). Primers (Table S1 in Supplementary Material) were selected according to previous references.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoblotting was performed according to our previous study (20, 24). Signal intensity was digitally quantified and normalized to actin or PCNA protein abundance.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Intestinal Microbiota-Derived GABA Mediates Interleukin-17 Expression during Enterotoxigenic Escherichia coli Infection

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

l-Glutamine and l-arginine protect against enterotoxigenic Escherichia coli infection via intestinal innate immunity in mice

et al. 2017

View full text Add to dashboard Cite

Dietary glutamine (Gln) or arginine (Arg) supplementation is beneficial for intestinal health; however, whether Gln or Arg may confer protection against Enterotoxigenic Escherichia coli (ETEC) infection is not known. To address this, we used an ETEC-infected murine model to investigate the protective effects of Gln and Arg. Experimentally, we pre-treated mice with designed diet of Gln or Arg supplementation prior to the oral ETEC infection and then assessed mouse mortality and intestinal bacterial burden. We also determined the markers of intestinal innate immunity in treated mice, including secretory IgA response (SIgA), mucins from goblet cells, as well as antimicrobial peptides from Paneth cells. ETEC colonized in mouse small intestine, including duodenum, jejunum, and ileum, and inhibited the mRNA expression of intestinal immune factors, such as polymeric immunoglobulin receptor (pIgR), cryptdin-related sequence 1C (CRS1C), and Reg3γ. We found that dietary Gln or Arg supplementation decreased bacterial colonization and promoted the activation of innate immunity (e.g., the mRNA expression of pIgR, CRS1C, and Reg3γ) in the intestine of ETEC-infected mice. Our results suggest that dietary arginine or glutamine supplementation may inhibit intestinal ETEC infection through intestinal innate immunity.

show abstract

Melatonin alters amino acid metabolism and inflammatory responses in colitis mice

et al. 2017

View full text Add to dashboard Cite

Inflammatory bowel disease is a chronic inflammatory dysfunction of the gastrointestinal tract. This study explored the hypothesis that melatonin has beneficial functions in the mouse model of colitis induced by dextran sodium sulfate (DSS), with a specific focus on the expression of intestinal inflammatory cytokines and the serum levels of amino acids. The results revealed that mice with melatonin supplementation had a reduction in weight loss and disease index induced by DSS treatment. Melatonin stifled the expression of colonic IL-17 in mice with DSS-induced colitis. Melatonin also lowered the serum levels of Asp, Ser, Met, and Leu (p < 0.05), but increased those of Glu and Cys (p < 0.05). Thus, melatonin treatment is promising and may function as a potential adjuvant therapy to alleviate the clinical symptoms of patients with inflammatory bowel disease.

show abstract

Proteome analysis for the global proteins in the jejunum tissues of enterotoxigenic Escherichia coli -infected piglets

Cited by 30 publications

References 36 publications

Intestinal Microbiota-Derived GABA Mediates Interleukin-17 Expression during Enterotoxigenic Escherichia coli Infection

Intestinal Microbiota-Derived GABA Mediates Interleukin-17 Expression during Enterotoxigenic Escherichia coli Infection

l-Glutamine and l-arginine protect against enterotoxigenic Escherichia coli infection via intestinal innate immunity in mice

Melatonin alters amino acid metabolism and inflammatory responses in colitis mice

Contact Info

Product

Resources

About