Molecular analyses of thyroid tumors for diagnosis of malignancy on fine-needle aspiration biopsies and for prognosis of invasiveness on surgical specimens

Rousset, Bernard; Ziercher, Léa; Borson‐Chazot, Françoise

doi:10.1016/j.ando.2011.03.017

Cited by 3 publications

(3 citation statements)

References 26 publications

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“…New technologies are now available to test the BRAF status in FNA sample. Perhaps, by detecting BRAF mutations preoperatively, surgeons would be able to decide which patients should undergo pCND and total thyroidectomy and which should undergo total thyroidectomy alone [ 102 , 103 ]. However, further studies are required.…”

Section: Patient Selectionmentioning

confidence: 99%

The Role of Prophylactic Central Neck Dissection in Differentiated Thyroid Carcinoma: Issues and Controversies

Wong

Lang

2011

Journal of Oncology

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Prophylactic central neck dissection (pCND) in differentiated thyroid carcinoma (DTC) is one of the most controversial surgical subjects in recent times. To date, there is little evidence to support the practice of pCND in patients with DTC undergoing total thyroidectomy. Although the recently revised American Thyroid Association (ATA) guideline has clarified many inconsistencies regarding pCND and has recommended pCND in “high-risk” patients, many issues and controversies surrounding the subject of pCND in DTC remain. The recent literature has revealed an insignificant trend toward lower recurrence rate in patients with DTC who undergo total thyroidectomy and pCND than those who undergo total thyroidectomy alone. However, this was subjected to biases, and there are concerns whether pCND should be performed by all surgeons who manage DTC because of increased surgical morbodity. Performing a unilateral pCND may be better than a bilateral pCND given its lower surgical morbidity. Further studies in this controversial subject are much needed.

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Section: Patient Selectionmentioning

confidence: 99%

The Role of Prophylactic Central Neck Dissection in Differentiated Thyroid Carcinoma: Issues and Controversies

Wong

Lang

2011

Journal of Oncology

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“…They revealed three gene lists which discriminated between histologically normal thyroid tissue and follicular neoplasms (421 genes), between FTCs and FTAs (94 genes) and between aggressive FTC and nonaggressive FTC (4 genes; NID2, TM7SF2, TRIM2, and GLTSCR2). Recently Rousset et al (Rousset et al, 2011) elucidated 19 genes to distinguish between malignant and benign thyroid tumours which can be applied for improved diagnostic testing using molecular methods.…”

Section: Rna Expression Microarrays In Thyroid Cancer Researchmentioning

confidence: 99%

“…Especially in cancer research, microarray technology has brought forth many new insights and has been widely applied for the elucidation of biological interrelations, effects, pathways and aetiology of cancers such as thyroid cancer (Kundel et al, 2010;Williams et al, 2011;Rousset et al, 2011;Cheng et al, 2011;Vierlinger et al, 2011). Microarrays have initiated a new era of research for scientists, with new challenges.…”

Section: Introductionmentioning

confidence: 99%

Principles and Application of Microarray Technology in Thyroid Cancer Research

Pulverer¹,

Noehammer²,

Vierlinger³

et al. 2012

Updates in the Understanding and Management of Thyroid Cancer

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A more sensitive platform for the detection of low-abundance BRAFV600E mutations

Jiang

Wang

et al. 2012

Mol Cell Biochem

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Identifying low-abundance mutations is important for the therapy and diagnose of cancer. Since the potential for tumor heterogeneity, the efficient detection of cancer-relevant mutations largely depends on the sensitivity of the methods employed. To confirm whether the mutation detection platforms affect the perceived prevalence of the BRAF(V600E) and its correlation with clinicopathologic features in papillary thyroid carcinomas (PTC), we compared Sanger Sequencing (SS), Pyrosequencing (PS), and a newly built allele-specific real-time PCR (AS-qPCR) apparatus for the detection of BRAF(V600E) in a Chinese cohort of conventional variant PTC. Accurate plasmid standards were built to assess the limit of detection of the three platforms. In this research, AS-qPCR has been found both the most sensitive and reliable at detecting mutation. The mutations detected by AS-qPCR which were not detected by SS or PS due to low abundance were confirmed by mutation enrichment platform COLD-PCR followed by SS. When analyzed by AS-qPCR, BRAF(V600E) was associated with a more aggressive phenotype. Our results indicate that the reported prevalence of the BRAF(V600E) mutations in PTC has been underestimated and more sensitive methods such as AS-qPCR should be applied in clinical settings.

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Molecular analyses of thyroid tumors for diagnosis of malignancy on fine-needle aspiration biopsies and for prognosis of invasiveness on surgical specimens

Cited by 3 publications

References 26 publications

The Role of Prophylactic Central Neck Dissection in Differentiated Thyroid Carcinoma: Issues and Controversies

The Role of Prophylactic Central Neck Dissection in Differentiated Thyroid Carcinoma: Issues and Controversies

Principles and Application of Microarray Technology in Thyroid Cancer Research

A more sensitive platform for the detection of low-abundance BRAFV600E mutations

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