Principles and Application of Microarray Technology in Thyroid Cancer Research

Pulverer, Walter; Noehammer, Christa; Vierlinger, Klemens; Weinhaeusel, Andreas

doi:10.5772/27894

Cited by 5 publications

(5 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If our target sites in the genes are found, they will form complementary bonding with the probes and emit fluorescence signal which can be read by specialized camera and computer systems. Nevertheless, we will discuss in more detail the main steps in measuring gene expression levels in a biological sample using DNA microarray technology [2,7]. This includes sample preparation and tagging, hybridization, washing, image acquisition and normalization.…”

Section: Experimental Principles Of Dna Microarraysmentioning

confidence: 99%

“…The reason for this is to confirm that there is no important similarity exists or to find a close match [3,7]. This can be performed using local alignment search algorithms such as LAlign and BLAST, which are fast and freely available.…”

Section: The Role Of Bioinformatics In Dna Microarraysmentioning

confidence: 99%

“…This was discussed in detail in this review in terms of methodology and different approaches used by researchers when dealing with different sources of DNA and RNA [7]. Other applications can include genotyping where scientists can detect single nucleotide polymorphisms (SNPs), which are type of genetic variation among people due to a difference in single nucleotide [32].…”

Section: Applications Of Dna Microar-raysmentioning

confidence: 99%

“…They managed to measure the gene expression of 48 Arabidopsis thaliana genes using cDNA derived from PCR products and printing the array using a constructed robot [6,7]. By the next decade, florescent detection has became part of the DNA array technology in which gave another advantage as the visualization of hybridized DNA was easier and read quickly by the system [2,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…By the next decade, florescent detection has became part of the DNA array technology in which gave another advantage as the visualization of hybridized DNA was easier and read quickly by the system [2,8,9]. Moreover, further research was carried on this remarkable technology and gradually, its protocols were improved such as the use of very long sequences in the beginning did not yield much success [7]. Thus, using shorter oligos of 25-60 bp has resulted in higher specificity as these were designed to bind to target sites in the gene that were most divergent from other sites or genes [6,9].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

DNA Microarray and Bioinformatics Technologies: A mini-review

Missoum¹

2018

Proc. Nat. Res. Soc.

View full text Add to dashboard Cite

This bibliographic review gives a concise overview of how DNA microarrays became one of the most important recent technologies. In brief, DNA sequences acting as probes are fixed on tiny slides in arrays to identify the presence of tagged fluorescently nucleic acids from a certain sample. The nucleic acids having complementary sequences with probes will hybridize on the array slides and because they are labeled, they are detected easily using specialized scanner and software. According to the way DNA microarrays are manufactured, there are three main types including spotted arrays, in situ synthesized arrays, and self-assembled arrays. Bioinformatics, which is the use of mathematical and computational methods, plays a crucial role in this technology as it is used for designing specific primers and probes suitable for each sample that is investigated. Moreover, although DNA microarrays are widely used to determine gene expression levels, they are also used in single nucleotide polymorphism (SNP) genotyping. For this application itself, more than one approach can be used including allele discrimination using hybridization, array primer extension assay (APEX), and Illumina infinium assay. The APEX approach was discussed in more detail as it was found that to be useful in screening for inherited genetic diseases such as Stargardt disease. Finally, this review was ended with short discussion of some DNA microarray limitations arising hybridization kinetics as well as difficulties in designing arrays for highly variable genomes.

show abstract

Section: Experimental Principles Of Dna Microarraysmentioning

confidence: 99%

Section: The Role Of Bioinformatics In Dna Microarraysmentioning

confidence: 99%