Molecular alterations of bone quality in sequesters of bisphosphonates-related osteonecrosis of the jaws

Olejnik, Cécile; Falgayrac, Guillaume; During, Alexandrine; Vieillard, Marie-Hélène; Maes, Jean-Michel; Cortet, Bernard; Penel, Guillaume

doi:10.1007/s00198-013-2514-3

Cited by 18 publications

(16 citation statements)

References 45 publications

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“…The Raman bands due to PMMA (a commonly used embedding resin when handling biological tissues) are ~1365 cm −1 (weak) and 1390 cm −1 (medium); thus, in all measurements obtained in embedded tissue, the PMMA contribution was spectrally subtracted, including the Raman images. We have previously utilized the 1375 cm −1 Raman band to describe PG concentration and distribution in bone, while the results presented in the current study show its utility in cartilage analysis.…”

Section: Discussionmentioning

confidence: 89%

Raman analysis of proteoglycans simultaneously in bone and cartilage

Gamsjaeger

Klaushofer

Paschalis

2014

J Raman Spectroscopy

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Bone and cartilage are connective tissues with distinct organic matrix (collagen and non‐collagenous proteins) composition facilitating their biological function. Proteoglycans (PGs), a member of the non‐collagenous proteins fulfill functions that are determined by both their core protein and their glycosaminoglycan chains. The purpose of the present study was to identify Raman bands that are representative of PG concentration and may be used in both bone and cartilage tissues. To achieve this goal, we analyzed a series of reference PGs and collagens, as well as turkey leg tendon to verify the laser polarization independency of the identified bands. Additionally, the applicability of these bands in both cartilage and bone tissue simultaneously was tested in a healthy femoral head by Raman imaging and hierarchical cluster analysis to describe the distribution of PGs at the micron level from articular cartilage to subchondral bone. The results of the study show that the Raman band ~1375 cm−1 can be used as a PGs marker band in both cartilage and bone. Moreover, articular cartilage has a lower content of organic matrix (mostly type II collagen), while the middle and deep transitional zone haves a higher concentration of PGs. The calcified cartilage is characterized by a lower content of PGs and total organic matrix (estimated from the integrated area of the amide III band). Copyright © 2014 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 89%

Raman analysis of proteoglycans simultaneously in bone and cartilage

Gamsjaeger

Klaushofer

Paschalis

2014

J Raman Spectroscopy

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“…Over the last decade several investigators have reported the usefulness of Raman spectroscopy in the diagnosis of a variety of bone diseases in humans, that has been summarized chronologically as a table in the supplementary information 14 – 22 . These reports range from studies looking at mineralization and structural deterioration of bone in osteoporosis 14 , 20 , structural deterioration of collagen of nails in osteoporosios 22 , monitoring treatment of osteoporosis 15 , 17 , and monitoring a major adverse reaction to the treatment of osteoporosis such as osteonecrosis of jaw 19 . Other diseases affecting the structure of bone such as osteomalacia 18 , and osteogenesis imperfecta 21 , 22 have also been studied using Raman spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Raman Spectroscopy detects changes in Bone Mineral Quality and Collagen Cross-linkage in Staphylococcus Infected Human Bone

Khalid

Bora

Ghaithi

et al. 2018

Sci Rep

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Diagnosis of osteomyelitis presents a formidable challenge. Lack of pathognomonic clinical sign(s) and diagnostic tests that can diagnose osteomyelitis at an early stage contribute to this difficulty. If the diagnosis is not made early, the disease becomes very difficult to eradicate and can lead to limb threatening and potentially life-threatening complications. Staphylococcus aureus is the most common organism causing osteomyelitis. Raman Spectroscopy provides information about molecular vibration that could potentially be harnessed as a spectral signature for cellular changes in specific pathologic conditions. In this study we describe a technique using Raman spectroscopy that could potentially be used to diagnose staphylococcal osteomyelitis. Human bone samples were co-cultured with Staphylococcus aureus (S. aureus) and the effects of bacterial growth on bone quality were then monitored using Raman spectroscopy. A major drop in the bone mineral quality and crystallinity was observed in the infected bones compared to the controls. S. aureus infection was also found to alter the collagen cross-linking. Our study shows that specific spectral signatures are present for the cause as well as the effect of staphylococcal osteomyelitis, opening the possibility of developing a useful diagnostic modality for early and rapid diagnosis of this condition.

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“…[66][67][68] Some model and treatment studies have gone further to examine relationships between changes in osteoporotic bone composition with alternations in bone material properties. 52,[69][70][71][72] For example, the mineral/matrix ratio and indentation modulus were found to be reduced in cancellous bone from the lumbar spine of female donors with osteoporosis.…”

Section: Osteoporosis Models and Treatment Effectsmentioning

confidence: 99%

Contributions of Raman spectroscopy to the understanding of bone strength

Mandair¹,

Morris²

2015

BoneKEy Reports

277

314

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Raman spectroscopy is increasingly commonly used to understand how changes in bone composition and structure influence tissue-level bone mechanical properties. The spectroscopic technique provides information on bone mineral and matrix collagen components and on the effects of various matrix proteins on bone material properties as well. The Raman spectrum of bone not only contains information on bone mineral crystallinity that is related to bone hardness but also provides information on the orientation of mineral crystallites with respect to the collagen fibril axis. Indirect information on collagen cross-links is also available and will be discussed. After a short introduction to bone Raman spectroscopic parameters and collection methodologies, advances in in vivo Raman spectroscopic measurements for animal and human subject studies will be reviewed. A discussion on the effects of aging, osteogenesis imperfecta, osteoporosis and therapeutic agents on bone composition and mechanical properties will be highlighted, including genetic mouse models in which structure-function and exercise effects are explored. Similarly, extracellular matrix proteins, proteases and transcriptional proteins implicated in the regulation of bone material properties will be reviewed.

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Molecular alterations of bone quality in sequesters of bisphosphonates-related osteonecrosis of the jaws

Abstract: The present data show that jaw bone quality can be altered with an overmineralization and ultrastructural modifications of apatitic mineral in bone sequesters of BP-related ONJ.

Cited by 18 publications

References 45 publications

Raman analysis of proteoglycans simultaneously in bone and cartilage

Raman analysis of proteoglycans simultaneously in bone and cartilage

Raman Spectroscopy detects changes in Bone Mineral Quality and Collagen Cross-linkage in Staphylococcus Infected Human Bone

Contributions of Raman spectroscopy to the understanding of bone strength

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