Molecular adaptation of an H7N3 wild duck influenza virus following experimental multiple passages in quail and turkey

Giannecchini, Simone; Clausi, Valeria; Trani, Livia Di; Falcone, E.; Terregino, Calogero; Toffan, Anna; Cilloni, Filippo; Matrosovich, Mikhail; Gambaryan, Alexandra; Bovin, Nicolai V.; Delogu, Mauro; Capua, Ilaria; Donatelli, Isabella; Azzi, Alberta

doi:10.1016/j.virol.2010.09.011

Cited by 30 publications

(34 citation statements)

References 50 publications

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“…Their NAs were both characterized by a Michaelis-Menten constant (K m ) of 32 M, as determined using the small fluorogenic substrate MUNANA (21). However, the activity of the short-stalk NA was dramatically reduced compared to that of the long-stalk variant, as measured by the elution time of the corresponding viruses from red blood cells of either chickens or turkeys (Ͼ20 h and Ͻ3.5 h for the wt and insNA viruses, respectively), in agreement with previously published studies (8,16). …”

supporting

confidence: 90%

Length Variations in the NA Stalk of an H7N1 Influenza Virus Have Opposite Effects on Viral Excretion in Chickens and Ducks

Hoffmann

Munier

Larcher

et al. 2012

J Virol

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A deletion of ϳ20 amino acids in the stalk of neuraminidase is frequently observed upon transmission of influenza A viruses from waterfowl to domestic poultry. A pair of recombinant H7N1 viruses bearing either a short-or long-stalk neuraminidase was genetically engineered. Inoculation of the long-stalk-neuraminidase virus resulted in a higher cloacal excretion in ducks and led conversely to lower-level oropharyngeal excretion in chickens, associated with a higher-level local immune response and better survival. Therefore, a short-stalk neuraminidase is a determinant of viral adaptation and virulence in chickens but is detrimental to virus replication and shedding in ducks.

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supporting

confidence: 90%

Length Variations in the NA Stalk of an H7N1 Influenza Virus Have Opposite Effects on Viral Excretion in Chickens and Ducks

Hoffmann

Munier

Larcher

et al. 2012

J Virol

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“…Thus, deletions in the stalk of the NA that affect its catalytic activity are commonly observed in H5 and H7 poultry influenza viruses but not in their putative duck precursors (2,8,31,33). Emergence of the NA stalk deletions as the first essential change in the duck influenza viruses during their experimental adaptation to poultry was confirmed in several independent studies (22,26,30,40). In addition to deletions in the NA, HA mutations leading to emergence of new glycosylation sites near the RBS of H5 and H7 influenza viruses correlate with virus isolation from poultry.…”

Section: Discussionmentioning

confidence: 78%

Receptor-Binding Profiles of H7 Subtype Influenza Viruses in Different Host Species

Gambaryan

Matrosovich

Philipp

et al. 2012

J Virol

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115

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“…Although the original source of the Italian LPAI H7N1 outbreak has never been identified, some of the early isolates in our study lack the molecular signatures that frequently appear during the adaptation of viruses from aquatic bird hosts to domestic poultry (72,73), including the stalk deletion in the NA protein and the additional glycosylation sites in the HA protein. Hence, it is possible that wild birds have been the source of this virus.…”

Section: Discussionmentioning

confidence: 90%

Emergence of a Highly Pathogenic Avian Influenza Virus from a Low-Pathogenic Progenitor

Monne

Fusaro

Nelson

et al. 2014

J Virol

129

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Avian influenza (AI) viruses of the H7 subtype have the potential to evolve into highly pathogenic (HP) viruses that represent a major economic problem for the poultry industry and a threat to global health. However, the emergence of HPAI viruses from low-pathogenic (LPAI) progenitor viruses currently is poorly understood. To investigate the origin and evolution of one of the most important avian influenza epidemics described in Europe, we investigated the evolutionary and spatial dynamics of the entire genome of 109 H7N1 (46 LPAI and 63 HPAI) viruses collected during Italian H7N1 outbreaks between March 1999 and February 2001. Phylogenetic analysis revealed that the LPAI and HPAI epidemics shared a single ancestor, that the HPAI strains evolved from the LPAI viruses in the absence of reassortment, and that there was a parallel emergence of mutations among HPAI and later LPAI lineages. Notably, an ultradeep-sequencing analysis demonstrated that some of the amino acid changes characterizing the HPAI virus cluster were already present with low frequency within several individual viral populations from the beginning of the LPAI H7N1 epidemic. A Bayesian phylogeographic analysis revealed stronger spatial structure during the LPAI outbreak, reflecting the more rapid spread of the virus following the emergence of HPAI. The data generated in this study provide the most complete evolutionary and phylogeographic analysis of epidemiologically intertwined high-and low-pathogenicity viruses undertaken to date and highlight the importance of implementing prompt eradication measures against LPAI to prevent the appearance of viruses with fitness advantages and unpredictable pathogenic properties. IMPORTANCEThe Italian H7 AI epidemic of 1999 to 2001 was one of the most important AI outbreaks described in Europe. H7 viruses have the ability to evolve into HP forms from LP precursors, although the mechanisms underlying this evolutionary transition are only poorly understood. We combined epidemiological information, whole-genome sequence data, and ultradeep sequencing approaches to provide the most complete characterization of the evolution of HPAI from LPAI viruses undertaken to date. Our analysis revealed that the LPAI viruses were the direct ancestors of the HPAI strains and identified low-frequency minority variants with HPAI mutations that were present in the LPAI samples. Spatial analysis provided key information for the design of effective control strategies for AI at both local and global scales. Overall, this work highlights the importance of implementing rapid eradication measures to prevent the emergence of novel influenza viruses with severe pathogenic properties.

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Molecular adaptation of an H7N3 wild duck influenza virus following experimental multiple passages in quail and turkey

Cited by 30 publications

References 50 publications

Length Variations in the NA Stalk of an H7N1 Influenza Virus Have Opposite Effects on Viral Excretion in Chickens and Ducks

Length Variations in the NA Stalk of an H7N1 Influenza Virus Have Opposite Effects on Viral Excretion in Chickens and Ducks

Receptor-Binding Profiles of H7 Subtype Influenza Viruses in Different Host Species

Emergence of a Highly Pathogenic Avian Influenza Virus from a Low-Pathogenic Progenitor

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