Molding a Silver Nanoparticle Template on Polydimethylsiloxane to Efficiently Capture Mammalian Cells

Abstract

Herein, a functional template made up of in situ synthesized silver nanoparticles (AgNPs) is prepared on polydimethylsiloxane (PDMS) for the spatial control of cell capture, where the residual Si-H groups in the PDMS matrix are used as reductants to reduce AgNO(3) for forming AgNPs. In virtue of microfluidic system, a one-dimensional array pattern of AgNPs is obtained easily. Further combining with plasma treatment, a two-dimensional array pattern of AgNPs could be achieved. The obtained PDMS-AgNPs composite is characterized in detail. The PDMS-AgNPs composite shows good antibacterial property in E. coli adhesion tests. The patterns possess hifi and high resolution (ca. 8 microm). Cell patterns with high efficiency and spatial selectivity are further formed with the aid of H-Arg-Gly-Asp-Cys-OH (RGDC) tetrapeptide which is grafted on the AgNPs template. Cells immobilized on the template show a good ability for adhesion, spreading, migration, and growth.

Silicones for Microfluidic Systems

“…Th e pattern on the PDMS stamp is transferred onto the substrate (glass, PDMS). • Deposition of metal nanoparticles (immobilization of functionalized species [104], in situ synthesis [105][106][107], electrochemical etching [108], self-assembling of colloids [109], sputter coating [110]). • Directed growth of carbon nanotubes on PDMS surface [111,112].…”

“…• Immobilization of biomolecules for biomolecular detection using immunoassays [35,75,[177][178][179][180] and enzymatic microreactors [181,182]. • Cell culture under controlled fl ow conditions on surfaces modifi ed with ligands important for cell adhesion and proliferation [35,73,76,105]. • Controlled formation of emulsions and double emulsions inside microfl uidic channels with chemically/topographically patterned surface for application in drug delivery [100,183].…”

A Simple Water‐Based Synthesis of Au Nanoparticle/PDMS Composites for Water Purification and Targeted Drug Release

“…[7,8] Cell adhesion and patterning, bio-electrochemical sensing, and antimicrobial materials combine specific metal nanoparticle properties with the bio-compatible nature of polymers, leading to advanced bio-functional materials. [9][10][11] The choice of polymer thus plays an important role in determining the functionality of the nanocomposite. In this context, polydimethylsiloxane (PDMS) is an attractive polymeric matrix due to its many favorable properties such as chemical inertness, biocompatibility, mechanical flexibility and stability, high dielectric constant and breakdown field, optical clarity in the visible and ultraviolet region and importantly, due to ease of processing.…”

“…[22] In this case, HAuCl 4 is reduced at the surface of cured PDMS in aqueous medium at room temperature over a period of several hours to days. Patterning of microelectrodes, [23] cell adhesion, [9] and polymer wettability [24] has been demonstrated using similar processes combined with oxygen plasma treatment. Ajayan and coworkers synthesized Au, Ag, and Pt nanoparticle/PDMS composite films by introducing the metal salt in organic solvents with the uncured PDMS and allowing the nanoparticles to reduce during curing.…”

Synthesis of gold-poly (dimethylsiloxane) nanocomposite through a polymer-mediated silver/gold galvanic replacement reaction

“…It has received the most attention due to its ease of preparation, low cost, good transparency and non-toxicity to biomolecules. Due to these properties, PDMS can be used directly for cell culturing [13,14]. In addition, PDMS has a low glass transition temperature (T g ), excellent flexibility, high thermal and oxidative stability, good hemo-and biocompatibility [15], properties that make it a very attractive polymer host for the fabrication of nanocomposites [16].…”