Molar Growth Yields as Evidence for Oxidative Phosphorylation in
            <i>Streptococcus faecalis</i>
            Strain 10C1

Smalley, A. J.; Jahrling, Peter B.; Demark, P. J. Van

doi:10.1128/jb.96.5.1595-1600.1968

Cited by 36 publications

(11 citation statements)

References 16 publications

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“…oxidative phosphorylation (). Oxidative phosphorylation has previously been reported for another facultative anaerobic bacterium, namely Enterococcus faecalis [32], on the basis of measurements of NADH oxidation by membrane fractions and indirectly by molar growth yields [33,34]. The latter investigators, however, did not supplement their medium with haemin or any blood derivative, and a publication by Bryan‐Jones and Whittenbury [35] could not confirm the findings.…”

Section: Discussionmentioning

confidence: 99%

Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions

Koebmann

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Solem

et al. 2008

Biotech and App Biochem

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Lactococcus lactis is known to be capable of respiration under aerobic conditions in the presence of haemin. In the present study the effect of respiration on ATP production during growth on different sugars was examined. With glucose as the sole carbon source, respiratory conditions in L. lactis MG1363 resulted in only a minor increase, 21%, in biomass yield. Since ATP production through substrate-level phosphorylation was essentially identical with and without respiration, the increased biomass yield was a result of energy-saving under respiratory conditions estimated to be 0.4 mol of ATP/mol of glucose. With maltose as the energy source, the increase in biomass yield amounted to 51% compared with an aerobic culture that lacked haemin. This higher ATP yield was obtained by redirecting pyruvate metabolism from lactate to acetate production, and from savings through respiration. However, even after subtracting these contributions, approx. 0.3 mol of ATP/mol of glucose remained unaccounted for. A similar response to respiratory conditions (0.2 mol of ATP/mol of glucose) was observed in a mutant that had a decreased glucose uptake rate during growth on glucose caused by disruption of the PTS(mannose) (glucose/mannose-specific phosphotransferase system). Amino acid catabolism could be excluded as the source of the additional ATP. Since mutants without a functional H+-ATPase produced less ATP under sugar starvation and respiratory conditions, the additional ATP yield appears to come partly from energy saved on proton pumping through the H+-ATPase due to respiration and partly from a reversed function of the H+-ATPase towards oxidative phosphorylation. These results may contribute to the design and implementation of carbon-efficient high-cell-density cultures of this industrially important species of bacterium.

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Section: Discussionmentioning

confidence: 99%

Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions

Koebmann

Blank

Solem

et al. 2008

Biotech and App Biochem

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“…It is not surprising that some homolactic bacteria also benefit from the presence of Oz; yields of biomass during aeration are often greater than in the absence of Oz. The effects are particularly noticeable at low concentrations of sugar [41,52,[86][87][88]. Growth rates of some homolactic LAB are faster in the presence of 02 than in its absence, especially if catalase is present to eliminate any HzO 2 formed [8,32].…”

Section: Advantages Of Aerobic Metabo-lismmentioning

confidence: 99%

Responses of lactic acid bacteria to oxygen

Condón

1987

FEMS Microbiology Letters

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Key words: Lactic acid bacteria; Oxygen SUMMARY 2. INTRODUCTIONA small number of flavoprotein oxidase enzymes are responsible for the direct interaction of lactic acid bacteria (LAB) with oxygen; hydrogen peroxide or water are produced in these reactions. In some cultures exposed to oxygen, hydrogen peroxide accumulates to inhibitory levels.Through these oxidase enzymes and NADH peroxidase, 02 and H202 can accept electrons from sugar metabolism, and thus have a sparing effect on the use of metabolic intermediates, such as pyruvate or acetaldehyde, as electron acceptors. Consequently, sugar metabolism in aerated cultures of LAB can be substantially different from that in unaerated cultures. Energy and biomass yields, end-products of sugar metabolism and the range of substrates which can be metabolised are affected.Lactic acid bacteria exhibit an inducible oxidative stress response when exposed to sublethal levels of H202. This response protects them if they are subsequently exposed to lethal concentrations of H202. The effect appears to be related to other stress responses such as heat-shock and is similar, in some but not all respects, to that previously reported for enteric bacteria.

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“…Although several lactic acid bacteria have been shown to be capable of utilizing 02 (6,8,11,20,23,26), they characteristically do not form catalase and possess only a low level of superoxide dismutase (19). In contrast to the typical catalase-negative lactic acid bacteria, there are a number of isolates from various genera which possess an atypical, nonheme catalase (15,26).…”

mentioning

confidence: 99%

Oxygen metabolism of catalase-negative and catalase-positive strains of Lactobacillus plantarum

Yousten¹,

Johnson²,

Salin³

1975

J Bacteriol

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Two catalase-negative strains of Lactobacillus plantarum and a strain producing the atypical, nonheme catalase were studied to determine if the ability to produce the atypical catalase conferred any growth advantage upon the producing strain. Both catalase-negative strains grew more rapidly than the catalase-positive strain under aerobic or anaerobic conditions in a glucose-containing, complex medium. Upon exhaustion of glucose from the medium, all three strains continued growth under aerobic but not under anaerobic conditions. The continued aerobic growth was accompanied by production of acetic acid in addition to the lactic acid produced during growth on glucose. Oxygen was taken up by exponential phase-cell suspensions grown on glucose when glucose or glycerol were used as substrates. Cells harvested from glucose-exhausted medium oxidized glucose, glycerol, and pyruvate. Oxygen utilization by a catalase-negative strain increased as did the specific activity of reduced nicotinamide adenine dinucleotide peroxidase during late growth in the glucose-exhausted medium. The catalase-positive strain and the catalase-negative strain tested both possessed low but readily detectable levels of superoxide dismutase throughout growth. The growth responses are discussed in terms of the presence of enzymes which would allow the cells to remove potentially damaging reduction products of O2.

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Molar Growth Yields as Evidence for Oxidative Phosphorylation in Streptococcus faecalis Strain 10C1

Cited by 36 publications

References 16 publications

Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions

Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions

Responses of lactic acid bacteria to oxygen

Oxygen metabolism of catalase-negative and catalase-positive strains of Lactobacillus plantarum

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