Moko Disease-Causing Strains of <i>Ralstonia solanacearum</i> from Brazil Extend Known Diversity in Paraphyletic Phylotype II

Albuquerque, Greecy Mirian Rodrigues; Santos, Liliana; Felix, Kátia Cilene S.; Rollemberg, Christtianno L.; Silva, Adriano Márcio Freire; Souza, Elineide Barbosa de; Cellier, Gilles; Prior, Philippe; Mariano, R. L. R.

doi:10.1094/phyto-12-13-0334-r

Cited by 40 publications

(37 citation statements)

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“…These lineages presumably developed when progenitors became geographically isolated and subsequently adapted to different environments and potential host plants (Denny, 2006). Isolates studied in Brazil from banana and Heliconia belong to phylotype II and sequevars IIA-6, IIA-24, IIA41, IIB-25, and to a new sequevar IIA53 (Albuquerque et al, 2014), showing the variability of the pathogen in Brazil. The results support the effectiveness of the egl gene in revealing relationships among strains.…”

Section: Introductionmentioning

confidence: 96%

Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management

et al. 2017

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Bacterial diseases of bananas and enset have not received, until recently, an equal amount of attention compared to other major threats to banana production such as the fungal diseases black leaf streak (Mycosphaerella fijiensis) and Fusarium wilt (Fusarium oxysporum f. sp. cubense). However, bacteria cause significant impacts on bananas globally and management practices are not always well known or adopted by farmers. Bacterial diseases in bananas and enset can be divided into three groups: (1) Ralstonia-associated diseases (Moko/Bugtok disease caused by Ralstonia solanacearum and banana blood disease caused by R. syzygii subsp. celebesensis); (2) Xanthomonas wilt of banana and enset, caused by Xanthomonas campestris pv. musacearum and (3) Erwinia-associated diseases (bacterial head rot or tip-over disease Erwinia carotovora ssp. carotovora and E. chrysanthemi), bacterial rhizome and pseudostem wet rot (Dickeya paradisiaca formerly E. chrysanthemi pv. paradisiaca). Other bacterial diseases of less widespread importance include: bacterial wilt of abaca, Javanese vascular wilt and bacterial fingertip rot (probably caused by Ralstonia spp., unconfirmed). This review describes global distribution, symptoms, pathogenic diversity, epidemiology and the state of the art for sustainable disease management of the major bacterial wilts currently affecting banana and enset.

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Section: Introductionmentioning

confidence: 96%

Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management

et al. 2017

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“…More recently, 37 isolates from banana and heliconia, mainly from the Northeast region, were classified as phylotype II. Sequevars 3, 4, 6, 24, 25 and 41 were detected, and a new sequevar 53 was identified using the egl and mutS genes, showing the high diversity of these isolates in Brazil (Albuquerque et al ., ). Overall, studies show there is evidence of high genetic variability in the Brazilian population of R .…”

Section: Introductionmentioning

confidence: 97%

Phylotype and sequevar variability of Ralstonia solanacearum in Brazil, an ancient centre of diversity of the pathogen

Santiago

Lopes²,

Caetano‐Anollés

et al. 2016

Plant Pathology

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The β‐proteobacterium Ralstonia solanacearum causes bacterial wilt of many plant species. Knowledge of phylotype and sequevar variability in populations of this microorganism is useful for implementing control measures, particularly host resistance. To this end, 301 isolates of R. solanacearum were collected from different geographic regions and hosts in Brazil. Their phylotype and sequevar characterization was used to determine the amount and distribution of phenetic and phylogenetic variability. Isolates were classified into phylotypes I (n = 48), clade 1; and phylotype II, clades 2–5. Phylotype II was divided into subclusters IIA (n = 112) and IIB (n = 141). Phylotype II was widely distributed, whereas phylotype I isolates were found in Central, Northern, and Northeastern regions of Brazil. There were 108 haplotypes identified among endoglucanase (egl) gene sequences from 301 isolates and 32 haplotypes among DNA repair (mutS) gene regions from 176 isolates. The egl and mutS sequence analyses identified eight known (1, 4, 7, 18, 27, 28, 41 and 50) and four new (54, 55, 56 and 57) sequevars. Phylotype IIB showed high diversity in sequevars and host range. Multiplex PCR, using primers specific to the Moko ecotype, characterized banana and long pepper isolates as sequevar 4 and 4/NPB, respectively. This constitutes the first report of the emergent ecotype IIB/4NPB in a new host, long pepper. The majority of sequevars were associated with geographic regions. This high variability of R. solanacearum in Brazil suggests use of host resistance to control bacterial wilt should be mainly focused by region.

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“…However, it was not possible to characterize most strains into sequevars, and, possibly, the banana strains belong to a not yet determined sequevar (PINHEIRO et al, 2011). This observation was also reported by Albuquerque et al (2014) that described a new sequevar associated to Moko, named IIA-53. Strains from eucalypt plants were not grouped into known sequevars neither (FONSECA et al, 2014).…”

Section: Genetic Diversity Of Ralstonia Solanacearum In Brazilmentioning

confidence: 84%

Occurrence and diversity of Ralstonia solanacearum populations in Brazil

et al. 2015

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ABSTRACT:Ralstonia solanacearum is a gram-negative soil-borne bacterium capable of infection of hundreds of vegetable species over more than 50 botanical families, causing bacterial wilt, except for bananas, when the disease is called Moko. It deserves special attention, from all other plant pathogenic bacteria, for its high phenotypic and genotypic plasticity, a characteristic that makes disease control extremely difficult. In this context, frequent and necessary surveys are conduct in the attempt of characterizing the prevailing strains of R. solanacearum in each region where the disease has been reported. However, knowledge about occurrence and diversity of R. solanacearum in Brazil is fragmented and in some cases, based on inconclusive studies with few strains, little representative of a given region. The need to obtain a greater picture guided this review. The occurrence of this bacterium in Brazilian States and the possible causes for its dissemination are presented, with emphasis on studies of genetic variability of populations of R. solanacearum in the country. The compiled results report a wide distribution of the bacterium in Brazil and great variability of its populations among locations. Partly due to the difficulty of detecting small titer of bacteria in samples, paucity of information about the origin of inoculum in certain regions is observed, as well as the need for detecting the presence of the pathogen in asymptomatic plants, potato tubers with latent infections, soil, and water, which are the major causes of bacterial dissemination into areas without any disease history.

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Moko Disease-Causing Strains of Ralstonia solanacearum from Brazil Extend Known Diversity in Paraphyletic Phylotype II

Cited by 40 publications

References 25 publications

Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management

Bacterial Diseases of Bananas and Enset: Current State of Knowledge and Integrated Approaches Toward Sustainable Management

Phylotype and sequevar variability of Ralstonia solanacearum in Brazil, an ancient centre of diversity of the pathogen

Occurrence and diversity of Ralstonia solanacearum populations in Brazil

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