Mogroside IIIE, a Novel Anti-Fibrotic Compound, Reduces Pulmonary Fibrosis through Toll-Like Receptor 4 Pathways

Tao, Lijun; Yang, Jinyu; Cao, Fengyan; Xie, Haifeng; Zhang, Mian; Gong, Yanqing; Zhang, Chaofeng

doi:10.1124/jpet.116.239137

Cited by 41 publications

(33 citation statements)

References 40 publications

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“…In the present study, we found that mogroside V treatment was effective at reducing the OVA‐induced activation of NF‐κB in the lungs of mice. In other studies, it was reported that both mogroside IIIE and mogroside V attenuate lipopolysaccharide‐induced lung inflammation and acute lung damage by reducing the activation of NF‐κB signaling pathways (Shi et al, ; Tao, Cao, et al, ; Tao, Yang, et al, ).…”

Section: Discussionmentioning

confidence: 98%

“…Recently, many studies revealed that mogroside V has some health‐promoting effects including antioxidant (Chen, Wang, Qi, & Xie, ), anti‐inflammatory (Di, Huang, & Ho, ), and anti‐pancreatic‐cancer activities (Liu, Dai, Dou, Ma, & Sun, ). Mogroside V also protects mice from chemically induced damage to lungs (Shi, Zheng, Wang, Liu, & Chen, ; Tao, Cao, Xu, Xie, & Zhang, ; Tao, Yang, et al, ), attenuates high‐fat‐diet–induced obesity (Qi, Chen, Zhang, & Xie, ) and nonalcoholic fatty liver disease in mice (Zhang et al, ), and improves metabolism in mice with type 2 diabetes (Zhang et al, ). Nevertheless, the antiasthmatic effect of mogroside V has not been assessed yet.…”

Section: Introductionmentioning

confidence: 99%

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Protective activity of mogroside V against ovalbumin‐induced experimental allergic asthma in Kunming mice

et al. 2019

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We investigated the antiasthmatic effect of mogroside V (Mog V) in mice with ovalbumin (OVA)‐induced asthma. Administration of Mog V effectively attenuated OVA‐induced airway hyperresponsiveness and reduced the number of inflammatory cells in bronchoalveolar lavage fluid (BALF). Histological examination showed that Mog V reduced the inflammatory infiltration of the lungs in the asthmatic mice. ELISAs suggested that Mog V effectively decreased the levels of IL‐4, IL‐5, and IL‐13 in BALF and serum levels of OVA‐specific IgE and IgG1 in the asthmatic mice. A quantitative reverse‐transcription PCR assay also indicated that Mog V decreased the mRNA levels of IL‐17A, IL‐23, and RORγt in the lungs of the asthmatic mice (the opposite effect on Foxp3 mRNA). Furthermore, Mog V significantly reduced the OVA‐induced activation of NF‐κB in the lungs. This study indicates that Mog V alleviates OVA‐induced inflammation in airways, and this effect is associated with a reduction in NF‐κB activation. Practical applications A traditional Chinese medicine herb has been reported to have a strong curative effect on asthma in clinical practice. Siraitia grosvenorii is known in China as a functional food product with the ability to improve lung function. Mogroside V is a triterpene glycoside isolated from S. grosvenorii. Nonetheless, the antiasthmatic effect of mogroside V has not been evaluated yet. The aim of this study was to investigate the antiasthmatic activity of mogroside V in mice with chemically induced asthma. The data from this study will provide some scientific evidence supporting wider use of S. grosvenorii in functional foods.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Protective activity of mogroside V against ovalbumin‐induced experimental allergic asthma in Kunming mice

et al. 2019

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“…34 Recently, emerging evidence showed that F I G U R E 6 The regulation role of miR-7 on Smad2 in LF. 35 39 TLR9 stimulation facilitated the proliferation and differentiation of cardiac fibroblasts through activating NF-κB pathway to protect hearts from cardiac rupture. B, Dual-luciferase reporter gene assay showed that miR-7 mimic reduced the luciferase activity of Smad2 3′UTR WT, and miR-7 inhibitor increased the luciferase activity of Smad2 3′UTR WT.…”

Section: Discussionmentioning

confidence: 99%

“…38 Mogroside IIIE protected against bleomycin-induced PF in vivo, and in vitro experiments further determined that MGIIIE suppressed fibroblast activation and collagen production through down-regulating TLR4/MyD88 MAPK signalling pathway. 39 TLR9 stimulation facilitated the proliferation and differentiation of cardiac fibroblasts through activating NF-κB pathway to protect hearts from cardiac rupture. 40 In this study, we Our data clearly determined that TLR9 agonist CpG-ODN remarkably up-regulated CCN2 and α-SMA expression levels, accelerated the collagen production and promoted the proliferation of LFs, resulting in the promotion of MF differentiation.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil extracellular traps activate lung fibroblast to induce polymyositis‐related interstitial lung diseases via TLR9‐miR‐7‐Smad2 pathway

Zhang

Jia

Zhang

et al. 2019

J Cellular Molecular Medi

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| INTRODUC TI ONPolymyositis (PM) is the common disease of idiopathic inflammatory myopathy that affects skeletal muscle, lung, heart, joints, etc. 1 Interstitial lung diseases (ILDs) is the most common complication which seriously affects the prognosis of PM patients. 2 A retrospective study showed the morbidity rate was 48.9% in patients with PM/dermatomyositis (DM)-related ILD in Chinese Han population. 3 Another study reported the mortality rate was 47.1% in myositis patients with ILD. 4 Based on the high morbidity and mortality rates, finding effective preventive and therapeutic methods is important for improving prognosis of patients with PM-related ILD. Interstitial lung diseases AbstractExcessive neutrophil extracellular trap (NET) formation may contribute to polymyositis (PM)-associated interstitial lung diseases (ILD), but the underlying mechanism is not fully revealed. In this study, we found that NET accelerated the progression of ILD and promoted pulmonary fibrosis (PF) in vivo. miR-7 expression was downregulated in lung tissue of PM group than control group, and NETs further decreased miR-7 expression. TLR9 and Smad2 were up-regulated in lung tissue of PM group than control group, and NETs further increased TLR9 and Smad2 expressions. In vitro experiments showed that PMA-treated NETs accelerated the proliferation of LF and their differentiation into myofibroblast (MF), whereas DNase I decreased the promotion effect of NETs. Neutrophil extracellular trap components myeloperoxidase (MPO) and histone 3 also promoted the proliferation and differentiation of LF. In addition, we demonstrated that TLR9 involved in the regulation of NETs on LF proliferation and differentiation, and confirmed the interaction between miR-7 and Smad2 in LF. Finally, miR-7-Smad2 pathway was confirmed to be involved in the regulation of TLR9 on LF proliferation and differentiation. Therefore, NETs promote PM-related ILD, and TLR9-miR-7-Smad2 signalling pathway is involved in the proliferation of LFs and their differentiation into MFs. K E Y W O R D Sdifferentiation, interstitial lung diseases, lung fibroblast, myofibroblast, neutrophil extracellular traps, polymyositis

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“…MG-III reduced pulmonary fibrosis and may have therapeutic potential for treating fibrosis (Tao et al, 2017).…”

mentioning

confidence: 96%

Hydrolyzation of mogrosides: Immobilized β‐glucosidase for mogrosides deglycosylation from Lo Han Kuo

Wang

Yang

Chen

et al. 2019

Food Science & Nutrition

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An immobilized enzyme system for bioconversion of Lo Han Kuo (LHK) mogrosides was established. β‐Glucosidase which was covalently immobilized onto the glass spheres exhibited a significant bioconversion efficiency from pNPG to pnitrophenol over other carriers. Optimum operational pH and temperature were determined to be pH 4 and 30°C. Results of storage stability test demonstrated that the glass sphere enzyme immobilization system was capable of sustaining more than 80% residual activity until 50 days, and operation reusability was confirmed for at least 10 cycles. The Michaelis constant (Km) of the system was determined to be 0.33 mM. The kinetic parameters, rate constant (K) at which Mogrosides conversion was determined, the τ50 in which 50% of mogroside V deglycosylation/mogroside IIIE production was reached, and the τ complete of complete mogroside V deglycosylation/mogroside IIIE production, were 0.044/0.017 min−1, 15.6/41.1 min, and 60/120 min, respectively. Formation of the intermediates contributed to the kinetic differences between mogroside V deglycosylation and mogroside IIIE formation.

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Mogroside IIIE, a Novel Anti-Fibrotic Compound, Reduces Pulmonary Fibrosis through Toll-Like Receptor 4 Pathways

Cited by 41 publications

References 40 publications

Protective activity of mogroside V against ovalbumin‐induced experimental allergic asthma in Kunming mice

Protective activity of mogroside V against ovalbumin‐induced experimental allergic asthma in Kunming mice

Neutrophil extracellular traps activate lung fibroblast to induce polymyositis‐related interstitial lung diseases via TLR9‐miR‐7‐Smad2 pathway

Hydrolyzation of mogrosides: Immobilized β‐glucosidase for mogrosides deglycosylation from Lo Han Kuo

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