Modulatory potential on immune system of a new alkaloid isolated from Coccinea cordifolia root

Roy, Saumendu Deb; Dash, Suvakanta; Dasgupta, Ramesh Kumari; Chakraborty, Jashabir

doi:10.1016/j.ijbiomac.2018.10.104

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…Alkaloids are essential nitrogenous chemicals with significant pharmacological activities that are mostly extracted from certain plants 1,2 . It has been found that alkaloids show efficacy in anti‐tumor, 3–5 anti‐inflammatory, 6 anti‐viral, 7 anti‐bacterial, 8 hypoglycemic, 9 regulation of the body's autoimmunity 10 and treatment of cardiovascular system diseases 11,12 . Some alkaloids have obvious biological activities and low toxicity 13,14 .…”

Section: Introductionmentioning

confidence: 99%

Investigation of steric hindrance effect on the interactions between four alkaloids and HSA by isothermal titration calorimetry and molecular docking

Lv,

Li,

Zhang

et al. 2024

J of Molecular Recognition

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The binding of four alkaloids with human serum albumin (HSA) was investigated by isothermal titration calorimetry (ITC), spectroscopy and molecular docking techniques. The findings demonstrated that theophylline or caffeine can bind to HAS, respectively. The number of binding sites and binding constants are obtained. The binding mode is a static quenching process. The effects of steric hindrance, temperature, salt concentration and buffer solution on the binding indicated that theophylline and HSA have higher binding affinity than caffeine. The fluorescence and ITC results showed that the interaction between HSA and theophylline or caffeine is an entropy‐driven spontaneous exothermic process. The hydrophobic force was the primary driving factor. The experimental results were consistent with the molecular docking data. Based on the molecular structures of the four alkaloids, steric hindrance might be a major factor in the binding between HSA and these four alkaloids. This study elucidates the mechanism of interactions between four alkaloids and HSA.

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