Modulatory effects of Crataeva nurvala bark against testosterone and N-methyl-N-nitrosourea-induced oxidative damage in prostate of male albino rats

Abstract

Background:Antioxidant properties of Crataeva nurvala bark contains a variety of the bioactive phytochemical constituents in medicinal plants which include flavonoids, phenolic compounds, tannins, anthracene derivatives, and essential oils. Components from Crataeva nurvala bark have been accounted to play an important role in scavenging free radicals generated by mutagens and carcinogens. Androgens are the key factors in either the initiation or progression of prostate cancer by inducing oxidative stress. In the present set of investigations, the antioxidative potential of Crataeva nurvala bark extract against androgen-mediated oxidative stress in male Wistar rats has been studied.Materials and Methods:Oxidative damage in prostate was induced in rats by the injection of testosterone (100 mg/kg body weight [bw]) for 3 days followed by injection of chemical carcinogen N-Methyl N-Nitroso Urea (50 mg/kg bw) for 1 week. The oxidative damage in prostate-induced rats were treated with the ethanolic extract of Crataeva nurvala bark (150 mg/kg bw) and testosterone injection (2 mg/ kg bw) was also continued through the experimental period of 4 months. The prostate tissue was dissected out for biochemical analysis of lipid peroxidation and enzymic-antioxidants viz. catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, and glutathione reductase; the non-enzymic antioxidants viz. reduced glutathione, and Vitamin C.Results:The results revealed that testosterone administration induced the oxidative stress in rat prostate; however, in drug (150 mg/kg bw) supplemented groups, a significant protective effect of Crataeva nurvala bark against testosterone-induced oxidative injury was recorded.Conclusion:Hence, the study reveals that constituents present in Crataeva nurvala bark impart protection against androgen-induced oxidative injury in prostate.

Crataeva nurvala Bark (Capparidaceae) Extract Modulates Oxidative Stress‐Related Gene Expression, Restores Antioxidant Enzymes, and Prevents Oxidative Stress in the Kidney and Heart of 2K1C Rats

“…Catalase and SOD activities were restored in 2K1C rats by the treatment of the Crataeva nurvala bark extract. A previous report also supports this finding that both catalase and SOD may be restored by Crataeva nurvala bark extract treatment [ 46 ]. Antioxidant enzymes are regulated by nuclear factor erythroid 2-related factor 2 (Nrf-2) which is a transcriptional factor activated against oxidative stress to stimulate tissue defense [ 50 ].…”

“…This result can be supported by a previous study that reported that the ethanol extract of Crataeva nurvala stem bark showed protection in cisplatin-induced nephrotoxicity and kidney dysfunction [ 45 ]. In another study, it was revealed that oxidative stress induced by N-methyl-N-nitrosourea and testosterone elevated the concentration of MDA, which was controlled by the bark extract of Crataeva nurvala [ 46 ].…”

Efeitos do fitoterápico Tribulus terrestris sobre a próstata do gerbilo da Mongólia (Meriones unguiculatus)

“…Estes modelos são programados para desenvolverem, por exemplo, tumores prostáticos, os quais permitem os estudos diretamente relacionados com o câncer de próstata ou as neoplasias intra-epiteliais prostáticas, mas falham quando o objetivo é estudar meios de quimioprevenção ou os agentes promocionais dessas condições . O composto tem sido empregado em protocolos que testam a capacidade de indução do câncer em curto prazo por agentes promotores (POLLARD & LUCKERT, 1986e 1987POLLARD et al, 1989;POLLARD, 1992;MCCORMICK et al, 1998;PEIXOTO et al, 2016) ou então em protocolos que testam a capacidade quimiopreventiva de compostos anticâncer, quando estes inibiriam a ação da MNU (BOONE et al, 1992;BANUDEVI et al, 2011;KUMAR et al, 2012;SANCHES et al, 2013).…”