MODULATORY EFFECT OF CYCLOSPORIN A ONTERT-BUTYL HYDROPEROXIDE–INDUCED OXIDATIVE DAMAGE IN HEPATOCYTES

Abstract

In the present work, we followed an in vitro protective action of cyclosporin A (CsA) against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in hepatocytes. Various parameters (cell viability, cytosolic calcium level, rhodamine 123 accumulation as indicator of mitochondrial membrane potential and alanine-aminotransferase leakage from cells) were measured as an index of cytotoxicity. Tert-butyl hydroperoxide (1 mM) significantly increased cytosolic Ca2+ and affected mitochondrial membrane potential. Pretreatment with cyclosporin A (0.5 microM) reduced t-BHP-induced cytosolic Ca2+ increase and ALT (alanine-aminotransferase) leakage, but had no protective effect on t-BHP-induced changes of mitochondrial membrane potential. Our data thus suggest that the mechanism of cytoprotection of CsA on the cytosolic Ca2+ changes and ALT leakage induced by t-BHP, does not directly correlate with protection of t-BHP-induced changes of mitochondrial membrane potential.

Hepatoprotective properties of extensively studied medicinal plant active constituents: Possible common mechanisms

“…In those articles, we looked for possible underlying common mechanisms of cytoprotection from molecular levels to whole animal studies of various structurally unrelated molecules. We found that several compounds experimentally showed some hepato-ameliorative properties and enumerated possible explanations (Canova et al, 2007;Cerny et al, 2009;Farghali & Masek, 1998;Farghali et al, 1991aFarghali et al, ,b, 1994Farghali et al, , 1996aFarghali et al, ,b, 1997Farghali et al, , 2000bFarghali et al, , 2002Farghali et al, , 2003Gasbarrini et al, 1992aGasbarrini et al, ,b, 1993Kmonickova et al, 2001;Lekic et al, 2011;Sakr et al, 1991a,b).…”

Modulatory effect of methanolic extract of Vernonia amygdalina (MEVA) on tert‐butyl hydroperoxide–induced erythrocyte haemolysis

“…As a pro‐oxidant, t‐BHP has been used in various studies as a model of oxidative stress . Its many effects on cell metabolism such as changes in calcium homeostasis and increase of lipid peroxidation or decrease of mitochondrial membrane potential have been established. Likewise, the effect of t‐BHP on hypertension and aging has been documented .…”

Protective Effects of Saponin Mixture, Isolated from <i>Astragalus monspessulanus</i> subsp. <i>monspessulanus</i> on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes

“…As a prooxidant, t-BuOH was widely used in experimental toxicology as a model of liver damage. There is a large body of scientific evidence showing its effects on changes in calcium homeostasis [2], on elevation of lipid peroxidation and decrease of mitochondrial membrane potential [3] [4].…”