Modulation of TRPV1 function by <i>Citrus reticulata</i> (tangerine) fruit extract for the treatment of sensitive skin

Zhou, Li-Dan; Lu, Yi-Na; Liu, Qing; Shi, Xue-Mei; Tian, Jun

doi:10.1111/jocd.15578

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…Fluo-8 TM /AM (AAT Bioquest, Inc., Sunnyvale, CA, USA) and Rhod-2/AM (Dojindo, Kumamoto, Japan) were used to analyze cytosolic and mitochondrial Ca 2+ levels, respectively [16,17]. Briefly, 1 × 10 6 cells/mL of C. parapsilosis cells were incubated with FK506 at concentrations of 0, 0.5, and 2 µg/mL, as described above, at 37 • C for 3 h. Next, the cells were washed twice with Krebs buffer (132 mM NaCl, 4 mM KCl, 1.4 mM MgCl 2 , 6 mM glucose, 10 mM HEPES, 10 mM NaHCO 3 , and 1 mM CaCl 2 ; pH 7.2; Sigma-Aldrich) containing 0.1% Pluronic F-127 (Molecular Probes, Eugene, OR, USA) and 1% bovine serum albumin (FUJIFILM Wako Pure Chemical Corporation).…”

Section: Measurement Of Ca 2+ Levels In Cytosol and Mitochondriamentioning

confidence: 99%

Tacrolimus (FK506) Exhibits Fungicidal Effects against Candida parapsilosis Sensu Stricto via Inducing Apoptosis

Cho,

Takada,

Odaka

et al. 2023

JoF

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Tacrolimus (FK506), an immunosuppressant and calcineurin inhibitor, has fungicidal effects. However, its fungicidal effect is thought to be limited to basidiomycetes, such as Cryptococcus and Malassezia, and not to ascomycetes. FK506 had no fungicidal effect on Candida albicans, C. auris, C. glabrata, C. guilliermondii, C. kefyr, C. krusei, and C. tropicalis (>8 µg/mL); however, C. parapsilosis was susceptible to it at low concentrations of 0.125–0.5 µg/mL. C. metapsilosis and C. orthopsils, previously classified as C. parapsilosis, are molecularly and phylogenetically closely related to C. parapsilosis, but neither species was sensitive to FK506. FK506 increased the mitochondrial reactive oxygen species production and cytoplasmic and mitochondrial calcium concentration and activated metacaspases, nuclear condensation, and DNA fragmentation, suggesting that it induced mitochondria-mediated apoptosis in C. parapsilosis. Elucidating why FK506 exhibits fungicidal activity only against C. parapsilosis will provide new information for developing novel antifungal drugs.

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Section: Measurement Of Ca 2+ Levels In Cytosol and Mitochondriamentioning

confidence: 99%

Tacrolimus (FK506) Exhibits Fungicidal Effects against Candida parapsilosis Sensu Stricto via Inducing Apoptosis

Cho,

Takada,

Odaka

et al. 2023

JoF

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“…SL modulates TRPV1 activity. According to previous article 26,29 , TRPV1 can be detected in the cytoplasm and surrounding the nucleus of HaCaT by immunofluorescence. To further clarify the antagonistic effect of SL against TRPV1 activation, we used immunocytochemistry detection to mark the presense of TRPV1 protein in HaCaT cells.…”

Section: Resultsmentioning

confidence: 97%

Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

Ma²,

Chen³

et al. 2023

Sci Rep

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Biosurfactants are attracting much interest due to their potential application as therapeutic agents in the medical and cosmetic field. Previous studies have demonstrated that biosurfactant such as sophorolipid (SL) exhibits immunomodulatory effects. In this article, we found the potential of sophorolipid for inhibiting histamine-induced itch and preliminarily explored its molecular basis. First, behavioral tests indicated that SL can remit the histamine-induced scratching behaviors of mice. Second, SL can suppress the the calcium influx induced by histamine, HTMT and VUF8430 in HaCaT cells. RT-PCR analysis showed that the histamine-induced upregulation of mRNA levels of phospholipase Cγ1, 1,4,5-trisphosphate receptor (IP3R), and protein kinase Cα can be inhibted by SL, suggesting that SL may impede the PLC/IP3R signaling pathway activated by histamine. In further tests, the capsaicin-induced calcium influx can also be inhibited by SL. The immunofluorescence and molecular docking analysis indicated that SL acts as an inhibitor of transient receptor potential vanilloid-1 (TRPV1) activation to decrease calcium influx against stimuli. In summary, these results revealed that SL may inhibit histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity. This paper indicates that SL can be a useful treatment for histamine-dependent itch.

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“…Sensitive skin (SS), characterized by abnormal and unpleasant sensations, such as stinging, burning, pain, pruritus and tingling, is a syndrome that can be normal or accompanied by erythema, high level of transepidermal water loss (TEWL) and neuronal reactivity confirmed by the lactic acid sting test (LAST) or capsaicin sting test through transient receptor potential vanilloid subtype 1 (TRPV1) [1][2][3]. With the development of sequencing technology, the relationship between SS and the skin microbiome has been analysed in several studies [4,5].…”

Section: Introductionmentioning

confidence: 99%

Correlation between the facial skin microbiome and sensitive skin using the 2bRAD‐M technique

Lu,

Cheng,

Shi

2024

Intern J of Cosmetic Sci

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ObjectiveThis study aimed to expound on the correlation between facial skin microbiome and sensitive skin (SS) using a novel sequencing technique.MethodsWe applied the 2bRAD sequencing for the microbiome, which enables accurate characterization of the low‐biomass microbiome at species resolution to profile facial skin microbes in SS and non‐SS groups. Further, the bacterial colonies were isolated and cultured from skin surfaces to study the pro‐inflammatory effect on human keratinocytes by ELISA.ResultsWe accordingly identified 1142 genera and 4436 strains. In the SS group, the proportions of Actinomyces and Microbotryomycetes were significantly increased, whereas that of Acidimicrobiia was decreased. Kruskal–Wallis analysis revealed significant differences in 11 genera and 35 species, among which the proportions of Dermabacter, Chryseobacterium, Rhodotorula and Peptoniphilus A were increased in the SS group. Analysis of the top 10 genera revealed increased proportions of Cutibacterium, Corynebacterium and Staphylococcus. Moreover, the proportion of Dermabacter hominis was significantly increased by 18.9‐fold in the SS group, whereas those of many Streptococcus strains were significantly decreased. Focus on the isolated bacterial colonies from skin surfaces, more yellow colonies were found in SS group when cultured in Tryptic Soy Broth medium for 48 h, and more interleukin‐8 was detected on keratinocytes after yellow colonies stimulation, such as S.capitis, M.luteus.ConclusionsThis study suggests that more SS‐associated microorganisms can be identified using the 2bRAD technique even with a small sample size. Dermabacter hominis and Chryseobacterium was firstly reported with a significantly increase in SS, and the S.capitis, as well as M.luteus, but not S.aureus, may be associated with skin inflammation.

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Modulation of TRPV1 function by Citrus reticulata (tangerine) fruit extract for the treatment of sensitive skin

Cited by 4 publications

References 23 publications

Tacrolimus (FK506) Exhibits Fungicidal Effects against Candida parapsilosis Sensu Stricto via Inducing Apoptosis

Tacrolimus (FK506) Exhibits Fungicidal Effects against Candida parapsilosis Sensu Stricto via Inducing Apoptosis

Sophorolipid inhibits histamine-induced itch by decreasing PLC/IP3R signaling pathway activation and modulating TRPV1 activity

Correlation between the facial skin microbiome and sensitive skin using the 2bRAD‐M technique

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