Modulation of steroidogenic gene expression and hormone production of H295R cells by pharmaceuticals and other environmentally active compounds

Developmental exposure to the organophosphorus flame retardant tris(1,3-dichloro-2-propyl) phosphate: Estrogenic activity, endocrine disruption and reproductive effects on zebrafish

“…In the present study, we measured transcription levels of genes along with the HPG axis and liver. However, production of steroids is a complex process with multiple sensitive control points, so the genes within the steroidogenesis pathway are not transcribed to the same extent and simple statistical correlations between mRNA expression and hormone production may not be expected (Gracia et al, 2007). To clarify the relationship between female steroid hormone levels and fecundity, a PCA was used to reduce the dimension of gene transcriptional profiles by transforming them into a small number of PCs (Ji et al, 2013).…”

The impact of long term exposure to phthalic acid esters on reproduction in Chinese rare minnow (Gobiocypris rarus)

“…However, there are many inconsistent associations between sex hormone levels and transcriptions of genes involved in steroidogenesis both in vitro and in vivo assays (Aoki et al, 2011;Borch et al, 2006;Lambrot et al, 2009;Liu et al, 2005). Therefore, it has been suggested that the production of sex hormones is a complex process with multiple sensitive control points, and the genes involved in steroidogenesis are not transcribed to the same degree and correlations between hormone production and mRNA expression are not always observed (Gracia et al, 2007). In this study, the DEHP treatment induced significant liver VTG gene transcription in male and female rare minnow.…”

Review: <i>Pharmaceutical and personal care products (PPCPs) as endocrine disrupting contaminants (EDCs) in South African surface waters</i>

“…The semi-synthetic macrolide antibiotic tylosin, which is used in veterinary medicine, has been shown to increase the expression of the aldosteronogenic gene (CYP11β2), and decrease the production of T and E 2 at a concentration of 3 mg/L in an H295R steroidogenic assay, showing that this chemical can serve as both an antiestrogenic and anti-androgenic EDC (Table 2; Gracia et al, 2007). In the same study, another macrolide antibiotic, erythromycin, showed an increase in the expression of CYP11β2 and a reduction in T production at a concentration of 3 mg/L, but caused increased production of E 2 and P in the assay (Table 2; Gracia et al, 2007). Exposure of erythromycin in a recombinant yeast oestrogen screen (YES) showed that this compound may be a minor mimic of E 2 in binding to the oestrogen receptor in a dose-dependent manner, therefore having oestrogenic effects (Archer et al unpublished).…”