Modulation of spermatozoon acrosome reaction

Vigil, Pilar; Orellana, Renán; Cortés, Manuel E.

doi:10.4067/s0716-97602011000200007

Cited by 21 publications

(17 citation statements)

References 103 publications

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“…Activation of GABA receptors may be of importance in both male and female reproduction centrally (Henderson 2007, Brunton et al 2014 and perhaps also peripherally. For example, sperm membranes express GABA receptors that are involved in initiating the acrosome reaction (Meizel 1997, Vigil et al 2011. With respect to female physiology, there is extensive metabolism of DHP to allopregnanolone during equine (Legacki et al 2016) and human pregnancy (Hill et al 2007), and allopregnanolone has been implicated in affective mood disorders in pregnant and cyclic women (Schiller et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Equine 5α-reductase activity and expression in epididymis

Corbin

Legacki

Ball

et al. 2016

Journal of Endocrinology

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The 5α-reductase enzymes play an important role during male sexual differentiation, and in pregnant females, especially equine species where maintenance relies on 5α-reduced progesterone, 5α-dihydroprogesterone (DHP). Epididymis expresses 5α-reductases but was not studied elaborately in horses. Epididymis from younger and older postpubertal stallions was divided into caput, corpus and cauda and examined for 5α-reductase activity and expression of type 1 and 2 isoforms by quantitative realtime polymerase chain reaction (qPCR). Metabolism of progesterone and testosterone to DHP and dihydrotestosterone (DHT), respectively, by epididymal microsomal protein was examined by thin-layer chromatography and verified by liquid chromatography tandem mass spectrometry (LC-MS/MS). Relative inhibitory potencies of finasteride and dutasteride toward equine 5α-reductase activity were investigated. Pregnenolone was investigated as an additional potential substrate for 5α-reductase, suggested previously from in vivo studies in mares but never directly examined. No regional gradient of 5α-reductase expression was observed by either enzyme activity or transcript analysis. Results of PCR experiments suggested that type 1 isoform predominates in equine epididymis. Primers for the type 2 isoform were unable to amplify product from any samples examined. Progesterone and testosterone were readily reduced to DHP and DHT, and activity was effectively inhibited by both inhibitors. Using epididymis as an enzyme source, no experimental evidence was obtained supporting the notion that pregnenolone could be directly metabolized by equine 5α-reductases as has been suggested by previous investigators speculating on alternative metabolic pathways leading to DHP synthesis in placenta during equine pregnancies.

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Section: Introductionmentioning

confidence: 99%

Equine 5α-reductase activity and expression in epididymis

Corbin

Legacki

Ball

et al. 2016

Journal of Endocrinology

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“…g-Amino butyric acid (GABA) and progesterone are among the compounds that induce AR [1]. AR is of utmost importance for sperm ovum interaction and fertilization.…”

Section: Introductionmentioning

confidence: 99%

In-vitro effect of γ-amino butyric acid and progesterone on sperm motility and acrosome reaction in patients with varicocele before and after varicocelectomy

Mahran¹,

Kholef²

2014

Human Andrology

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Purpose g-Amino butyric acid (GABA) and progesterone play a role in the induction of acrosome reaction (AR) and sperm motility. Patients with varicocele may show abnormalities in the sperm count, motility, and AR. This study aims to evaluate the effect of GABA and progesterone on sperm motility and AR in patients with varicocele before and after varicocelectomy. Patients and methods Forty-two infertile patients with varicocele and oligozoospermia and/or asthenozoospermia were included in the study. All participants underwent full assessment of history, general and local clinical examination, scrotal Doppler ultrasonography, and conventional semen analysis. Semen samples were divided into three equal parts and double washed with Ham's F-10 medium alone as a control or a medium containing GABA (2.5 mmol/l) or progesterone (2.5 mmol/l) separately. The effect of GABA and progesterone on sperm motility and AR was evaluated. Four to 6 months after varicocelectomy, conventional semen analysis and the semen preparation procedure were repeated. ResultsIn patients with varicocele, the addition of GABA or progesterone led to a significant improvement in sperm motility and AR compared with samples treated with HAM's F-10 (control). After varicocelectomy, samples treated with HAM's F-10 and GABA or progesterone showed a significant improvement in sperm motility and AR compared with samples treated with HAM's F-10 only. Sperm motility and AR in samples treated with GABA or progesterone after varicocelectomy were significantly higher than those treated with GABA or progesterone before varicocelectomy. Conclusion GABA and progesterone improve sperm motility and induce AR in patients with varicocele. Sperm motility and AR showed significant improvement after varicocelectomy in response to GABA and progesterone compared with prevaricocelectomy.

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“…During its development, this cell loses its gene transcription ability; therefore, nongenomic signaling pathways are the only ones available. The fact that steroid hormone receptors in both spermatozoa and neurons are uncommonly alike in mammals represents another advantage, suggesting that both cell types possess a similar capacity to react to the same environmental hormone changes (Meizel 2004), for example, showing comparable responses to the interactions between steroid hormones and the GABAergic system Vigil, Orellana, and Corteś 2011). GABA together with progesterone (neuroactive steroid) would influence mood swings in women undergoing hormone replacement therapy by inducing a negative mood (Andreen et al 2009); in addition, it triggers the acrosomic reaction (AR) in human spermatozoa Vigil, Orellana, and Corteś 2011).…”

Section: Brain Developmental Windowmentioning

confidence: 99%

“…The fact that steroid hormone receptors in both spermatozoa and neurons are uncommonly alike in mammals represents another advantage, suggesting that both cell types possess a similar capacity to react to the same environmental hormone changes (Meizel 2004), for example, showing comparable responses to the interactions between steroid hormones and the GABAergic system Vigil, Orellana, and Corteś 2011). GABA together with progesterone (neuroactive steroid) would influence mood swings in women undergoing hormone replacement therapy by inducing a negative mood (Andreen et al 2009); in addition, it triggers the acrosomic reaction (AR) in human spermatozoa Vigil, Orellana, and Corteś 2011). Estradiol, on the other hand, would present the opposite effect together with GABA, both at the CNS and in spermatozoa, leading to a state of euthymia and inhibiting AR, respectively (Andreen et al 2009;Vigil, Orellana, and Corteś 2011).…”

Section: Brain Developmental Windowmentioning

confidence: 99%

“…GABA together with progesterone (neuroactive steroid) would influence mood swings in women undergoing hormone replacement therapy by inducing a negative mood (Andreen et al 2009); in addition, it triggers the acrosomic reaction (AR) in human spermatozoa Vigil, Orellana, and Corteś 2011). Estradiol, on the other hand, would present the opposite effect together with GABA, both at the CNS and in spermatozoa, leading to a state of euthymia and inhibiting AR, respectively (Andreen et al 2009;Vigil, Orellana, and Corteś 2011).…”

Section: Brain Developmental Windowmentioning

confidence: 99%

See 1 more Smart Citation

Influence of Sex Steroid Hormones on the Adolescent Brain and Behavior: An Update

Vigil

Del

Carrera³

et al. 2016

Linacre Q

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This review explains the main effects exerted by sex steroids and other hormones on the adolescent brain. During the transition from puberty to adolescence, these hormones participate in the organizational phenomena that structurally shape some brain circuits. In adulthood, this will propitiate some specific behavior as responses to the hormones now activating those neural circuits. Adolescence is, then, a critical "organizational window" for the brain to develop adequately, since steroid hormones perform important functions at this stage. For this reason, the adolescent years are very important for future behaviors in human beings. Changes that occur or fail to occur during adolescence will determine behaviors for the rest of one's lifetime. Consequently, understanding the link between adolescent behavior and brain development as influenced by sex steroids and other hormones and compounds is very important in order to interpret various psycho-affective pathologies.Lay Summary: The effect of steroid hormones on the development of the adolescent brain, and therefore, on adolescent behavior, is noticeable. This review presents their main activational and organizational effects. During the transition from puberty to adolescence, organizational phenomena triggered by steroids structurally affect the remodeling of brain circuits. Later in adulthood, these changes will be reflected in behavioral responses to such hormones. Adolescence can then be seen as a fundamental "organizational window" during which sex steroids and other hormones and compounds play relevant roles. The understanding of the relationship between adolescent behavior and the way hormones influence brain development help understand some psychological disorders.

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Modulation of spermatozoon acrosome reaction

Cited by 21 publications

References 103 publications

Equine 5α-reductase activity and expression in epididymis

Equine 5α-reductase activity and expression in epididymis

In-vitro effect of γ-amino butyric acid and progesterone on sperm motility and acrosome reaction in patients with varicocele before and after varicocelectomy

Influence of Sex Steroid Hormones on the Adolescent Brain and Behavior: An Update

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