Modulation of sleep-courtship balance by nutritional status in Drosophila

Duhart, José M.; Baccini, Victoria; Zhang, Yanan; Machado, Daniel Rodrigues; Koh, Kyunghee

doi:10.7554/elife.60853

Cited by 19 publications

(15 citation statements)

References 70 publications

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“…Besides, P2 neurons, which include FB interneurons, regulate chronic isolation evoked sleep loss [ 31 ]. Moreover, courtship-regulator P1 neurons activate T1 neurons and modulate sleep/courtship balance based on the nutritional status [ 32 ]. Taking all the information mentioned above into account, we consider that arousal signals related to aggression, courtship, nutrition, and vision converge into the PB-FB pathway including FB interneurons to regulate arousal.…”

Section: Discussionmentioning

confidence: 99%

Interneurons of fan-shaped body promote arousal in Drosophila

Kato

Tomita²,

Kume³

2022

PLoS ONE

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Sleep is required to maintain physiological functions and is widely conserved across species. To understand the sleep-regulatory mechanisms, sleep-regulating genes and neuronal circuits are studied in various animal species. In the sleep-regulatory neuronal circuits in Drosophila melanogaster, the dorsal fan-shaped body (dFB) is a major sleep-promoting region. However, other sleep-regulating neuronal circuits were not well identified. We recently found that arousal-promoting T1 dopamine neurons, interneurons of protocerebral bridge (PB) neurons, and PB neurons innervating the ventral part of the FB form a sleep-regulatory circuit, which we named “the PB-FB pathway”. In the exploration of other sleep-regulatory circuits, we found that activation of FB interneurons, also known as pontine neurons, promoted arousal. We then found that FB interneurons had possible connections with the PB-FB pathway and dFB neurons. Ca2+ imaging revealed that FB interneurons received excitatory signals from the PB-FB pathway. We also demonstrated the possible role of FB interneurons to regulate dFB neurons. These results suggested the role of FB interneurons in sleep regulation.

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Section: Discussionmentioning

confidence: 99%

Interneurons of fan-shaped body promote arousal in Drosophila

Kato

Tomita²,

Kume³

2022

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Besides, P2 neurons, which include FB interneurons, regulate chronic isolation evoked sleep loss 34 . Moreover, courtship-regulator P1 neurons activate T1 neurons and modulate sleep/courtship balance based on the nutritional status 35 . Taking the abovementioned into account, we consider that arousal signals related to aggression, courtship, nutrition, and vision converge into the PB-FB pathway and pontine neurons to regulate arousal.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, P2 neurons, which include FB interneurons, regulate chronic isolation evoked sleep loss 34 . Moreover, courtshipregulator P1 neurons activate T1 neurons and modulate sleep/courtship balance based on the nutritional status 35 .…”

Section: Discussionmentioning

confidence: 99%

Interneurons of fan-shaped body promote arousal in Drosophila

Kato

Tomita

Kume

2022

Preprint

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Sleep is required to maintain physiological functions and is widely conserved across species. To understand the sleep-regulatory mechanisms, sleep-regulating genes and neuronal circuits are studied in various animal species. In the sleep-regulatory neuronal circuits in Drosophila melanogaster, the dorsal fan-shaped body (dFB) is a major sleep-promoting region. However, other sleep-regulating neuronal circuits were not well identified. We recently found a novel sleep-regulatory circuit consisting of arousal-promoting T1 dopamine neurons and protocerebral bridge (PB) neurons innervating the ventral part of the FB, which we named “the PB-FB pathway”. However, the post-synaptic target of the PB-FB pathway was still unknown. To identify it, we performed anterograde tracing, immunohistochemistry, and Ca2+ imaging analysis and found that the PB-FB pathway projects to FB interneurons, also known as pontine neurons. Besides, we found that cholinergic pontine neurons promote arousal. Moreover, we indicated that pontine neurons form an anatomical connection with sleep-promoting dFB neurons. Together, we showed that pontine neurons receive excitatory signals from the PB-FB pathway and cholinergic pontine neurons promote arousal. These results completed one of the output pathways from the PB-FB pathway.

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“…Regarding the interaction between sleep and aggression, it has been shown that aggressive behaviors are suppressed in sleep-deprived male flies, and the changes are mediated by octopamine signaling (Kayser et al, 2015). Interestingly, Duhart and colleagues have recently reported that T1 neurons act downstream of courtship-and sleep-regulating P1 neurons to modulate nutrition-dependent sleep-courtship balance in male flies (Duhart et al, 2020). Elucidation of the mechanisms controlling the activity of T1 neurons may provide a novel link between sleep, aggression and courtship.…”

Section: Discussionmentioning

confidence: 99%

Protocerebral Bridge Neurons That Regulate Sleep in Drosophila melanogaster

et al. 2021

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The central complex is one of the major brain regions that control sleep in Drosophila. However, the circuitry details of sleep regulation have not been elucidated yet. Here, we show a novel sleep-regulating neuronal circuit in the protocerebral bridge (PB) of the central complex. Activation of the PB interneurons labeled by the R59E08-Gal4 and the PB columnar neurons with R52B10-Gal4 promoted sleep and wakefulness, respectively. A targeted GFP reconstitution across synaptic partners (t-GRASP) analysis demonstrated synaptic contact between these two groups of sleep-regulating PB neurons. Furthermore, we found that activation of a pair of dopaminergic (DA) neurons projecting to the PB (T1 DA neurons) decreased sleep. The wake-promoting T1 DA neurons and the sleep-promoting PB interneurons formed close associations. Dopamine 2-like receptor (Dop2R) knockdown in the sleep-promoting PB interneurons increased sleep. These results indicated that the neuronal circuit in the PB, regulated by dopamine signaling, mediates sleep-wakefulness.

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Modulation of sleep-courtship balance by nutritional status in Drosophila

Cited by 19 publications

References 70 publications

Interneurons of fan-shaped body promote arousal in Drosophila

Interneurons of fan-shaped body promote arousal in Drosophila

Interneurons of fan-shaped body promote arousal in Drosophila

Protocerebral Bridge Neurons That Regulate Sleep in Drosophila melanogaster

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