Modulation of Rice Leaf Angle and Grain Size by Expressing OsBCL1 and OsBCL2 under the Control of OsBUL1 Promoter

Jang, Seonghoe; Cho, Jwa Yeong; Do, Gyung-Ran; Kang, Yeeun; Li, Hsing-Yi; Song, Jaeeun; Kim, Ho-Youn; Kim, Beom-Gi; Hsing, Yue‐Ie C.

doi:10.3390/ijms22157792

Cited by 8 publications

(8 citation statements)

References 55 publications

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“…OsARF6 controls flag leaf angle and grain size by responding to auxin [ 12 ]. In addition, OsBUL1 encodes a basic helix–loop–helix (bHLH) transcriptional factor that regulates grain size and flag leaf angle [ 28 ]. Nevertheless, the molecular and cellular mechanisms underlying grain size and flag leaf angle remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

LG5, a Novel Allele of EUI1, Regulates Grain Size and Flag Leaf Angle in Rice

Liu

Wang

et al. 2023

Plants

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Grain size and flag leaf angle are two important traits that determining grain yield in rice. However, the mechanisms regulating these two traits remain largely unknown. In this study, a rice long grain 5 (lg5) mutant with a large flag leaf angle was identified, and map-based cloning revealed that a single base substitution followed by a 2 bp insertion in the LOC_Os05g40384 gene resulted in larger grains, a larger flag leaf angle, and higher plant height than the wild type. Sequence analysis revealed that lg5 is a novel allele of elongated uppermost internode-1 (EUI1), which encodes a cytochrome P450 protein. Functional complementation and overexpression tests showed that LG5 can rescue the bigger grain size and larger flag leaf angle in the Xiushui11 (XS) background. Knockdown of the LG5 transcription level by RNA interference resulted in elevated grain size and flag leaf angle in the Nipponbare (NIP) background. Morphological and cellular analyses suggested that LG5 regulated grain size and flag leaf angle by promoting cell expansion and cell proliferation. Our results provided new insight into the functions of EUI1 in rice, especially in regulating grain size and flag leaf angle, indicating a potential target for the improvement of rice breeding.

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Section: Introductionmentioning

confidence: 99%

LG5, a Novel Allele of EUI1, Regulates Grain Size and Flag Leaf Angle in Rice

Liu

Wang

et al. 2023

Plants

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“…A study using transgenic rice and Arabidopsis thaliana showed that high expression of OsBCL1 and OsBCL2 in the pulvinus of rice leaf significantly promoted GA 3 biosynthesis, increasing LA via promoting cell elongation rather than cell division. OsBCL1 and OsBCL2 are homologs of OsBC1 , which encodes a bHLH transcription factor [ 20 ]. An earlier study on the relationship between GAs and BRs in rice found that exogenous GAs significantly reduce LA.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to IAA, rice mutants with deficient SL synthesis also have increased LA [ 18 ], and SLs may be involved in regulating LA with other phytohormones [ 19 ]. GAs have a dual function: endogenous GAs usually promote the increase of LA [ 20 ], while exogenous GAs have the opposite effect [ 21 ]. Compared with the above phytohormones, BRs are at the core of LA regulation.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with the above phytohormones, BRs are at the core of LA regulation. They play a role in promoting the increase in LA in maize and rice [ 22 , 23 ], and can also interact with a variety of phytohormones and participate in the regulation of LA [ 19 , 20 , 24 ]. In addition, a recent study on maize mutant Semidwarf3 ( Sdw3 ) confirmed that ethylene also plays an important role in the regulation of LA [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic Investigation and RNA-seq of KN1 Involved in Leaf Angle Formation in Maize (Zea mays L.)

Wu,

Zhang,

Zhuang

et al. 2024

IJMS

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Leaf angle (LA) is one of the core agronomic traits of maize, which controls maize yield by affecting planting density. Previous studies have shown that the KN1 gene is closely related to the formation of maize LA, but its specific mechanism has not been fully studied. In this study, phenotype investigation and transcriptomic sequencing were combined to explore the mechanism of LA changes in wild type maize B73 and mutant kn1 under exogenous auxin (IAA) and abscisic acid (ABA) treatment. The results showed that the effect of exogenous phytohormones had a greater impact on the LA of kn1 compared to B73. Transcriptome sequencing showed that genes involved in IAA, gibberellins (GAs) and brassinosteroids (BRs) showed different differential expression patterns in kn1 and B73. This study provides new insights into the mechanism of KN1 involved in the formation of maize LA, and provides a theoretical basis for breeding maize varieties with suitable LA.

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“…OsILI1 is ubiquitously expressed in rice, whereas the highest expression of OsILI1 was observed in the lamina joint [ 12 ]. OsBUL1 ( O. sativa brassinosteroid upregulated 1-like1), an AtPRE homolog in rice, is preferentially expressed in the lamina joint where it controls cell elongation and positively affects leaf angles [ 31 , 32 ]. FaPRE1 was proposed as a ripening-associated gene and showed a rapid increase in expression in the receptacle during fruit enlargement [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of PRE Genes in Moso Bamboo (Phyllostachys edulis)

Zheng

Shin

Lin

et al. 2023

IJMS

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Basic helix–loop–helix (bHLH)/HLH transcription factors are involved in various aspects of the growth and development of plants. Here, we identified four HLH genes, PePRE1-4, in moso bamboo plants that are homologous to Arabidopsis PRE genes. In bamboo seedlings, PePRE1/3 were found to be highly expressed in the internode and lamina joint by using quantitative RT-PCR analysis. In the elongating internode of bamboo shoots, PePRE genes are expressed at higher levels in the basal segment than in the mature top segment. Overexpression of PePREs (PePREs-OX) in Arabidopsis showed longer petioles and hypocotyls, as well as earlier flowering. PePRE1 overexpression restored the phenotype due to the deficiency of AtPRE genes caused by artificial micro-RNA. PePRE1-OX plants showed hypersensitivity to propiconazole treatment compared with the wild type. In addition, PePRE1/3 but not PePRE2/4 proteins accumulated as punctate structures in the cytosol, which was disrupted by the vesicle recycling inhibitor brefeldin A (BFA). PePRE genes have a positive function in the internode elongation of moso bamboo shoots, and overexpression of PePREs genes promotes flowering and growth in Arabidopsis. Our findings provided new insights about the fast-growing mechanism of bamboo shoots and the application of PRE genes from bamboo.

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Modulation of Rice Leaf Angle and Grain Size by Expressing OsBCL1 and OsBCL2 under the Control of OsBUL1 Promoter

Cited by 8 publications

References 55 publications

LG5, a Novel Allele of EUI1, Regulates Grain Size and Flag Leaf Angle in Rice

LG5, a Novel Allele of EUI1, Regulates Grain Size and Flag Leaf Angle in Rice

Phenotypic Investigation and RNA-seq of KN1 Involved in Leaf Angle Formation in Maize (Zea mays L.)

Identification and Characterization of PRE Genes in Moso Bamboo (Phyllostachys edulis)

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