Phenotypic Investigation and RNA-seq of KN1 Involved in Leaf Angle Formation in Maize (Zea mays L.)

Abstract

Leaf angle (LA) is one of the core agronomic traits of maize, which controls maize yield by affecting planting density. Previous studies have shown that the KN1 gene is closely related to the formation of maize LA, but its specific mechanism has not been fully studied. In this study, phenotype investigation and transcriptomic sequencing were combined to explore the mechanism of LA changes in wild type maize B73 and mutant kn1 under exogenous auxin (IAA) and abscisic acid (ABA) treatment. The results showed that the effect of exogenous phytohormones had a greater impact on the LA of kn1 compared to B73. Transcriptome sequencing showed that genes involved in IAA, gibberellins (GAs) and brassinosteroids (BRs) showed different differential expression patterns in kn1 and B73. This study provides new insights into the mechanism of KN1 involved in the formation of maize LA, and provides a theoretical basis for breeding maize varieties with suitable LA.

Genetic Analysis and Fine Mapping of QTL for the Erect Leaf in Mutant mths29 Induced through Fast Neutron in Wheat

“…Research has revealed that during the initial stages of tissue differentiation, the preligule zone emerges at the junction between the leaf blade and the leaf sheath. Subsequently, the mature lamina joint is established through the ongoing proliferation and differentiation of cells [ 11 ]. The aberration in the lamina joint structure typically involves the inhibition of longitudinal cell elongation in the adaxial region (proximal to the leaf primordium) and an increase in the division of sclerotic cells in the abaxial region (distant from the leaf primordium), consequently resulting in the formation of erect leaves [ 12 , 13 ].…”