Modulation of RBC volume distributions by oxidants (phenazine methosulfate and tert-butyl hydroperoxide): Role of Gardos channel activation

Lisovskaya, I. L.; Shcherbachenko, Irina M.; Volkova, R. I.; Tikhonov, Vladimir P.

doi:10.1016/j.bioelechem.2008.04.008

Cited by 10 publications

(7 citation statements)

References 36 publications

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“…Previous studies reported that PS translocation to RBC surface can be induced by elevation of intracellular Ca 2+ concentration, 41,42 which in turn is exerted by ROS, known to activate specific and nonspecific ion channels in RBCs 41,43 . Since RBC storage is associated with OS, as noted above, we examined whether the reversal of PS externalization by rejuvenation is related to intracellular ROS and Ca 2+ levels in stored RBCs.…”

Section: Resultsmentioning

confidence: 94%

Rejuvenation treatment of stored red blood cells reverses storage‐induced adhesion to vascular endothelial cells

et al. 2009

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Section: Resultsmentioning

confidence: 94%

Rejuvenation treatment of stored red blood cells reverses storage‐induced adhesion to vascular endothelial cells

et al. 2009

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“…Accumulation of GSSG and reduction in GSH was not associated with any substantial changes in the intracellular ATP or NADPH (Sass et al, 1965;D'Alessandro et al, 2013). Finally, redox state of RBCs may be expressed as the ability to tolerate oxidative challenge (Lisovskaya et al, 2008;Sinha et al, 2015) which differs between individual RBCs as well.…”

Section: Redox State and Metabolismmentioning

confidence: 99%

Heterogeneity of Red Blood Cells: Causes and Consequences

et al. 2020

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Mean values of hematological parameters are currently used in the clinical laboratory settings to characterize red blood cell properties. Those include red blood cell indices, osmotic fragility test, eosin 5-maleimide (EMA) test, and deformability assessment using ektacytometry to name a few. Diagnosis of hereditary red blood cell disorders is complemented by identification of mutations in distinct genes that are recognized "molecular causes of disease." The power of these measurements is clinically wellestablished. However, the evidence is growing that the available information is not enough to understand the determinants of severity of diseases and heterogeneity in manifestation of pathologies such as hereditary hemolytic anemias. This review focuses on an alternative approach to assess red blood cell properties based on heterogeneity of red blood cells and characterization of fractions of cells with similar properties such as density, hydration, membrane loss, redox state, Ca 2+ levels, and morphology. Methodological approaches to detect variance of red blood cell properties will be presented. Causes of red blood cell heterogeneity include cell age, environmental stress as well as shear and metabolic stress, and multiple other factors. Heterogeneity of red blood cell properties is also promoted by pathological conditions that are not limited to the red blood cells disorders, but inflammatory state, metabolic diseases and cancer. Therapeutic interventions such as splenectomy and transfusion as well as drug administration also impact the variance in red blood cell properties. Based on the overview of the studies in this area, the possible applications of heterogeneity in red blood cell properties as prognostic and diagnostic marker commenting on the power and selectivity of such markers are discussed.

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“…Blood is subjected to oxidative stress during intermittent hypoxia (26,34). According to previous studies (21,22), some oxidants activated the Ca 2ϩ -sensitive K ϩ channel (Gardos channel) of erythrocytes, subsequently increasing K ϩ efflux through the plasma membrane and ultimately leading to the cell shrinkage, cytoskeletal rigidity, and breakdown of phosphatidylserine (PS) asymmetry. Elevated oxidative stress also enhanced Ca 2ϩ influx by activated nonselective cation channels, further stimulating the scramblase that facilitated the translocation of PS to the outer leaflet of erythrocyte membrane (16,17).…”

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confidence: 99%

Hypoxic exercise training causes erythrocyte senescence and rheological dysfunction by depressed Gardos channel activity

Mao¹,

Wang

2011

Journal of Applied Physiology

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Despite enhancing cardiopulmonary and muscular fitness, the effect of hypoxic exercise training (HE) on hemorheological regulation remains unclear. This study investigates how HE modulates erythrocyte rheological properties and further explores the underlying mechanisms in the hemorheological alterations. Twenty-four sedentary males were randomly divided into hypoxic (HE; n = 12) and normoxic (NE; n = 12) exercise training groups. The subjects were trained on 60% of maximum work rate under 15% (HE) or 21% (NE) O(2) condition for 30 min daily, 5 days weekly for 5 wk. The results demonstrated that HE 1) downregulated CD47 and CD147 expressions on erythrocytes, 2) decreased actin and spectrin contents in erythrocytes, 3) reduced erythrocyte deformability under shear flow, and 4) diminished erythrocyte volume changed by hypotonic stress. Treatment of erythrocytes with H(2)O(2) that mimicked in vivo prooxidative status resulted in the cell shrinkage, rigidity, and phosphatidylserine exposure, whereas HE enhanced the eryptotic responses to H(2)O(2). However, HE decreased the degrees of clotrimazole to blunt ionomycin-induced shrinkage, rigidity, and cytoskeleton breakdown of erythrocytes, referred to as Gardos effects. Reduced erythrocyte deformability by H(2)O(2) was inversely related to the erythrocyte Gardos effect on the rheological function. Conversely, NE intervention did not significantly change resting and exercise erythrocyte rheological properties. Therefore, we conclude that HE rather than NE reduces erythrocyte deformability and volume regulation, accompanied by an increase in the eryptotic response to oxidative stress. Simultaneously, this intervention depresses Gardos channel-modulated erythrocyte rheological functions. Results of this study provide further insight into erythrocyte senescence induced by HE.

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Modulation of RBC volume distributions by oxidants (phenazine methosulfate and tert-butyl hydroperoxide): Role of Gardos channel activation

Cited by 10 publications

References 36 publications

Rejuvenation treatment of stored red blood cells reverses storage‐induced adhesion to vascular endothelial cells

Rejuvenation treatment of stored red blood cells reverses storage‐induced adhesion to vascular endothelial cells

Heterogeneity of Red Blood Cells: Causes and Consequences

Hypoxic exercise training causes erythrocyte senescence and rheological dysfunction by depressed Gardos channel activity

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