Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve

Abstract

To investigate the effects of administration of raffinose and encapsulated Bifidobacterium breve JCM 1192 T cells on the rat cecal microbiota, in a preclinical synbiotic study groups of male WKAH/Hkm Slc rats were fed for 3 weeks with four different test diets: basal diet (group BD), basal diet supplemented with raffinose (group RAF), basal diet supplemented with encapsulated B. breve (group CB), and basal diet supplemented with both raffinose and encapsulated B. breve (group RCB). The bacterial populations in cecal samples were determined by fluorescence in situ hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP). B. breve cells were detected only in the RCB group and accounted for about 6.3% of the total cells as determined by FISH analysis. B. breve was also detected only in the RCB group by T-RFLP analysis. This was in contrast to the CB group, in which no B. breve signals were detected by either FISH or T-RFLP. Increases in the sizes of the populations of Bifidobacterium animalis, a Bifidobacterium indigenous to the rat, were observed in the RAF and RCB groups. Principalcomponent analysis of T-RFLP results revealed significant alterations in the bacterial populations of rats in the RAF and RCB groups; the population in the CB group was similar to that in the control group (group BD). To the best of our knowledge, these results provide the first clear picture of the changes in the rat cecal microbiota in response to synbiotic administration.

Metabolism of Four α-Glycosidic Linkage-Containing Oligosaccharides by Bifidobacterium breve UCC2003

“…1), corroborated by the comparative genome analysis presented below (see Fig. 2), and on other reports in the literature regarding raffinose utilization by bifidobacteria, raffinose-type sugar utilization appears to be an ubiquitous property of bifidobacteria, supporting previous publications on the prebiotic potential of (some of) these sugars (65)(66)(67)(68). Although transcription of the raf gene cluster of B. breve is induced in the presence of either raffinose, melibiose, or stachyose, it has been shown that the raffinose system in Streptococcus pneumoniae is upregulated in the presence of raffinose but not melibiose, suggesting differential regulatory mechanisms (69).…”

Prebiotic Effect of Lacto-N-biose I on Bifidobacterial Growth

“…Non-digestible food ingredients that promote the growth and/or activity of probiotics are termed prebiotics. Several oligosaccharides, such as raffinose, 2,3) fructo-oligosaccharide, 4,5) and galacto-oligosaccharide, [6][7][8] are known to function as prebiotics for bifidobacteria, and thus are specifically termed bifidus (or bifidogenic) factors.…”

Screening Cellulolytic Bacteria from the Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity

“…Based on the log phase of growth patterns, it can be seen specific growth rate (μ) of isolate bacteria KE-B6 that is equal to 0377 / hr and a generation (g) of 1.84 hours. This value is very important to predict the amount of biomass and the product to be produced (Waites et al, 2001;Madigan, 2009;Dinoto et al, 2006;Dinoto et al, 2013). Phase lag or adjustment phase of the cell with the environment (medium) does not occur in the growth of isolate bacteria KE-B6, is due to the adjustment phase have been occured when the starter in the fermentation for 22 hours on a rotary shaker before being mixed in media production.…”

“…Culture production medium is then taken 1.5 ml for centrifuged at 3000 rpm for 10 minutes. At this stage it was also determined specific growth rate and generation time (Waites et al, 2001, Dinoto et al, 2006Dinoto et al, 2013).…”